Chronic cervicitis associated with human papillomavirus and markers of inflammation in women of reproductive age


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To investigate the incidence of chronic cervicitis (CC), detection rates of various types of human papillomavirus (HPV), the significance of the viral load, expression of mRNA genes involved in regulating the cell cycle, apoptosis, immunity, matrix metalloproteinases, and the methylation of the WIF1 gene promoter region among patients with CC associated with HPV infection. Material and methods. The study comprised 202 patients aged from 25 to 49 years. A comprehensive clinical and laboratory examination included patient history taking, gynecological examination, colposcopy, HPV testing, liquid-based cytology and targeted cervical biopsy (according to indications), vaginal smear microscopy, assessment of vaginal microbiocenosis, analysis of the transcriptional profile of KI67 genes KI67, CDKN2A, BCL2, PGR, IFNAR1, IL1RN, CCND1, EGFR, IL1B, IL10, IL18, TNFA, GATA3, TLR4, CD68, TGFB1, CXCL5, CXCL10 CXCL13, mMp8, mMp9, and the determination of the methylation level of the WIF1 gene promoter region. Results. Among patients with cervical pathology, 25.7% were found to have HPV-associated CC. The most commonly detected types were 16, 33, 35, and 66. The presence of HPV infection in endocervical scrapings was associated with a 1.8- and 2.4-fold higher expression of mRNA of CDKN2A/ p16 gene and IL10, respectively (p < 0.05). The inflammatory process was accompanied by a 1.5 to 2.9 - fold increase in the expression of mRNA of IFNAR1, IL1B, IL10, TNFA, TLR4, CD68, TGFB1 and MMP9 genes (p <0.05). In the study cohort, the methylation of the WIF1 gene was either not observed or weakly expressed. Conclusion. The genes IFNAR1, IL1B, IL10, TNFA, TLR4, CD68, TGFB1, and MMP9, may be suggested as markers for CC. The presence of HPV contributes to an increase in the expression of mRNA of the CDKN2A/p16 genes and the immunosuppressive IL10.

Full Text

Restricted Access

About the authors

Armine S. Amirkhanyan

V.I. Kulakov NMRC for OG&P of Minzdrav of Russia

Email: 07062005@mail.ru
PhD student at the Research and Outpatient Department

Giuldana R. Bairamova

V.I. Kulakov NMRC for OG&P of Minzdrav of Russia

Email: bayramova@mail.ru
MD, clinical care supervisor at Research and Outpatient Department

Vsevolod I. Kiselev

V.I. Kulakov NMRC for OG&P of Minzdrav of Russia

Email: vkis10@mail.ru
Dr.Bio.Sci., professor, corr. member of the RAS, deputy director of the Institute of Oncology and Mammology

Vera N. Prilepskaya

V.I. Kulakov NMRC for OG&P of Minzdrav of Russia

Email: VPrilepskaya@mail.ru
MD, professor, deputy director

Andrey A. Poloznikov

D. Rogachev NMRC for PHO&I of Minzdrav of Russia

Email: andrey.poloznikov@fccho-moscow.ru
PhD in Chemistry, head of the Laboratory of Biochemistry and Enzymology

Olga V. Burmenskaya

V.I. Kulakov NMRC for OG&P of Minzdrav of Russia

Email: o_bourmenskaya@oparina4.ru
Dr.Bio.Sci., head of the Laboratory of Oncological Genetics

Irina O. Babkina

D. Rogachev NMRC for PHO&I of Minzdrav of Russia

Email: iobabkina@gmail.com
junior researcher at the Laboratory of Biochemistry and Enzymology

Alexandra V. Asaturova

V.I. Kulakov NMRC for OG&P of Minzdrav of Russia

PhD, senior researcher at the 1st Pathology Department

References

  1. Серов В.Н., Сухих Г.Т., Прилепская В.Н., Радзинский В.Е., ред. Руководство по амбулаторно-поликлинической помощи в акушерстве и гинекологии. М.: ГЭОТАР-Медиа; 2016. 1136с.
  2. Centers for Disease Control and Prevention. Incidence, prevalence, and cost of sexually transmitted infections in the United States. February 2013.
  3. Амирханян А. С., Прилепская В.Н., Байрамова Г.Р., Бурменская О.В., Костава М.Н., Асатурова А.В. Хронический цервицит: современные возможности диагностики и лечения. Акушерство и гинекология. 2018; 4: 22-7.
  4. Ollendorff A.T. Cervicitis. Updated: Feb 09, 2017.
  5. World Health Organization. Comprehensive cervical cancer control. A guide to essential practice. 2nd ed. Geneva: WHO; 2014. Available at: http//www.who. int/repro ductivehealth/ publications/ cancers/cervical-cancer-guide/en
  6. Jayakumar N.K. Cervicitis: how often is it non-specific! J. Clin. Diagn. Res. 2015; 9(3): EC11-2. https://dx.doi.org/10.7860/JCDR/2015/11594.5673.
  7. Burd E.M. Human papillomavirus laboratory testing: the changing paradigm. Clin. Microbiol. Rev. 2016; 29(2): 291-319. https://dx.doi.org/10.1128/ CMR.00013-15.
  8. Quaas J., Reich O., Frey Tirri B., Kuppers V. Explanation and use of the colposcopy terminology of the IFCPC (International Federation for Cervical Pathology and Colposcopy) Rio 2011. Geburtshilfe Frauenheilkd. 2013; 73(9): 904-7. https://dx.doi.org/10.1055/s-0033-1350824.
  9. Bourmenskaya O., Bayramova G., Nepsha O., Rebrikov D., Trofimov D., Muravieva V., Sukhikh G. Vaginal smear TNF-alpha, IL18, and GATA3 mRNA levels correlate with local inflammation. Int. J. Biomed. 2014; 4(4): 204-8.
  10. Vasiljevic N., Scibior-Bentkowska D., Brentnall A.R., Cuzick J., Lorincz A.T. Credentialing of DNA methylation assays for human genes as diagnostic biomarkers of cervical intraepithelial neoplasia in high-risk HPV positive women. Gynecol. Oncol. 2014; 132(3): 709-14. https://dx.doi.org/10.1016/j. ygyno.2014.02.001.
  11. Kan Y.Y., Liou Y.L., Wang H.J., Chen C.Y., Sung L.C., Chang C.F., Liao C.I. PAX1 methylation as a potential biomarker for cervical cancer screening. Int. J. Gynecol. Cancer. 2014; 24(5): 928-34. https://dx.doi.org/10.1097/ IGC.0000000000000155.
  12. Arbyn M., Tommasino M., Depuydt C., Dillner J. Are 20 human papillomavirus types causing cervical cancer? J. Pathol. 2014; 234(4): 431-5. https://dx.doi. org/10.1002/path.4424.
  13. Piroozmand A., Mostafavi Zadeh S.M., Madani A., Soleimani R., Nedaeinia R., Niakan M. et al. The association of high risk human papillomaviruses in patients with cervical cancer: an evidence based study on patients with squamous cell dysplasia or carcinoma for evaluation of 23 human papilloma virus genotypes. Jundishapur J. Microbiol. 2016; 9(4): e32728. https://dx.doi.org/10.5812/ jjm.32728.
  14. Сухих Г.Т., Ашрафян Л.А., Байрамова Г.Р., Бабкина И.О., Чернова В.Ф., Осипьянц А.И., Королькова А.И., Полозников А.А., Асфарова Г.Р., Муллабаева С.М., Коган Е.А., Муйжнек Е.Л., Друх В.М., Киселев В.И. Метилирования гена WIF 1 при цервикальных плоскоклеточных интра-эпителиальных поражениях. Акушерство и гинекология. 2017; 5: 114-23.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2019 Bionika Media