Prediction of pre-eclampsia on the couts of CD-16 negative monocytes
- Authors: Boris D.A.1, Volgina N.E.1, Krasnyi A.M.1,2, Tyutyunnik V.L.1, Kan N.E.1
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Affiliations:
- National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation
- N.K. Koltzov Institute of Developmental Biology of Russian Academy of Sciences
- Issue: No 7 (2019)
- Pages: 49-55
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248541
- DOI: https://doi.org/10.18565/aig.2019.7.49-55
- ID: 248541
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Abstract
Objective of the study. To investigate the relative counts of peripheral blood monocyte subsets in women with normal pregnancy and pre-eclampsia. Materials and methods. 48 patients were involved to the study. The main group included 36 pregnant women separated on 2 subgroups, 20pregnant women with mild pre-eclampsia and 12pregnant women with severe preeclampsia. The comparison group included 16 pregnant women with a physiological course of pregnancy. The leukocyte fraction was isolated from peripheral blood samples, the ratio of classical (CD14++CD16+HLA-DR+), intermediate (CD14++CD16 HLA-DR+) and non-classical (CD14+CD16++HLA-DR+) monocytes was determined using flow cytofluorimetry. The relative counts of monocyte subsets (%) was determined how the number of monocytes in each subsets to the total number of monocytes. Results. The study of peripheral blood monocyte showed that, the relative counts of classical CD16-negative monocytes in peripheral blood is inversely correlated with the severity of pre-eclampsia. The counts of classical monocytes in the control group was 56,5% in women with mild pre-eclampsia 40,65% р=0,01, in women with severe pre-eclampsia 17,4% р=0,001. ROC analysis showed very good diagnostic value when comparing control group and mild pre-eclampsia AUC=0,81 (95% CI 0,63-0,98) sensitivity 86%, specificity 80% and excellent diagnostic value when comparing control group and severe pre-eclampsia AUC=0,96 (95% CI 0,87-1) sensitivity 83%, specificity 100%. The counts of intermediate and non-classical monocytes was increased in the groups with mild pre-eclampsia and severe pre-eclampsia, while significant differences were observed only when comparing severe pre-eclampsia with the control group in both subsets. Conclusion. The study showed a significant discrepancy in the counts of classical monocytes between the control group and mild pre-eclampsia - 15.9%, and between the control group and severe pre-eclampsia - 55.1%. The findings can be used in the diagnosis of pre-eclampsia in early pregnancy in risk groups.
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About the authors
Daiana A. Boris
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation
Email: dayana_boris@mail.ru
postgraduate student
Nadegda E. Volgina
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation
Email: n_volgina@oparina4.ru
scientific researcher of the cytology laboratory
Aleksey M. Krasnyi
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation; N.K. Koltzov Institute of Developmental Biology of Russian Academy of SciencesPhD, the head of the cytology laboratory
Victor L. Tyutyunnik
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation
Email: tioutiounnik@mail.ru
PhD, MD, the head of the obstetric physiological department
Natalia E. Kan
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation
Email: kan-med@mail.ru
PhD, MD, the head of the obstetric department
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