Efficiency of maturation of oocytes obtained from cancer patients during ovariectomy


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To study in vitro maturation of oocytes obtained from cancer patients during ovariectomy. Subjects and methods. The investigation enrolled 24 patients aged 16 to 42 years who received cancer treatment. During surgery, partial or complete ovariectomy was performed, oocyte-cumulus complexes were removed from the ovarian medulla after ovariectomy, the obtained cells were matured, the oocytes were fertilized, and embryos were cultured. The rates of oocyte maturation, fertilization, and fragmentation and blastocyst formation were estimated. Results. A total of 287 oocyte-cumulus complexes were obtained. The rates of oocyte maturation, fertilization, and fragmentation were 41.4, 20.0, and 100%, respectively; the rate of blastocyst formation was 27.3%. Genetic analysis of the trophectoderm of blastocysts revealed that the latter contained a euploid chromosome set. Conclusion. Despite the fact that the rate of in vitro maturation of oocytes obtained from the ovarian medulla is low, the method is promising for preserving the reproductive potential in cancer patients.

Full Text

Restricted Access

About the authors

E. V Kovalskaya

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: kovalskaya.evgeniya@gmail.com
embryologist, 1st Department of Gynecology

A. O Kirillova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: stasia.kozyreva@gmail.com
PhD, embryologist, 1st Department of Gynecology

E. S Bunyaeva

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: es_bunyaeva@mail.ru
post-graduate student, 1st Department of Gynecology

G. N Khabas

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: khabas@list.ru
Ph.D., head of the Department of Innovative Oncology and Gynecology

N. S Kamaletdinov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: sunsh86@mail.ru
embryologist, 1st Department of Gynecology

T. A Nazarenko

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: t.nazarenko@mail.ru
Doctor of Medical Sciences, Professor, head of Institute of Reproductive Medicine

A. N Abubakirov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nondoc555@yahoo.com
PhD, head of the 1st Department of Gynecology

References

  1. Donnez J., Dolmans M.M. Ovarian cortex transplantation: 60 reported live births bring the success and worldwide expansion of the technique towards routine clinical practice. J. Assist. Reprod. Genet. 2015; 32: 1167-70
  2. Abir R., Feinmesser M., Yaniv I., Fisch B., Cohen I.J., Ben-Haroush A., Meirow D., Felz C., Avigad S. Occasional involvement of the ovary in Ewing sarcoma. Hum. Reprod. 2010; 25: 1708-12.
  3. Abir R., Aviram A., Feinmesser M., Stein J., Yaniv I., Parnes D., Ben-Haroush A., Meirow D., Rabizadeh E., Fisch B. Ovarian minimal residual disease in chronic myeloid leukaemia. Reprod. Biomed. Online. 2014; 28: 255-60.
  4. Dolmans M.M., Luyckx V., Donnez J., Andersen C.Y., Greve T. Risk of transferring malignant cells with transplanted frozen-thawed ovarian tissue. Fertil. Steril. 2013; 99: 1514-22.
  5. Park C.W., Lee S.H., Yang K.M., Lee I.H., Lim K.T., Lee K.H., Kim T.J. Cryopreservation of in vitro matured oocytes after ex vivo oocyte retrieval from gynecologic cancer patients undergoing radical surgery. Clin. Exp. Reprod. Med. 2016; 43(2): 119-25.
  6. Kedem A., Yerushalmi G.M., Brengauz M., Raanani H., Orvieto R., Hourvitz A., Meirow D. Outcome of immature oocytes collection of 119 cancer patients during ovarian tissue harvesting for fertility preservation. J. Assist. Reprod. Genet. 2018; 35(5): 851-6.
  7. Fasano G., Dechene J., Antonacci R., Biramane J., Vannin A.S., Van Langendonckt A., Devreker F., Demeestere I. Outcomes of immature oocytes collected from ovarian tissue for cryopreservation in adult and prepubertal patients. Reprod. Biomed. Online. 2017; 34(6): 575-82.
  8. Shirasawa H., Terada Y. In vitro maturation of human immature oocytes for fertility preservation and research material. Reprod. Med. Biol. 2017; 16(3): 258-67.
  9. Prasath E.B., Chan M.L., Wong W.H., Lim C.J., Tharmalingam M.D., Hendricks M., Loh S.F., Chia Y.N. First pregnancy and live birth resulting from cryopreserved embryos obtained from in vitro matured oocytes after oophorectomy in an ovarian cancer patient. Hum. Reprod. 2014; 29: 276-8.
  10. Uzelac P.S., Delaney A.A., Christensen G.L., Bohler H.C., Nakajima S.T. Live birth following in vitro maturation of oocytes retrieved from extracorporeal ovarian tissue aspiration and embryo cryopreservation for 5 years. Fertil. Steril. 2015; 104: 1258-60.
  11. Segers I., Mateizel I., Van Moer E., Smitz J., Tournaye H., Verheyen G., De Vos M. In vitro maturation (IVM) of oocytes recovered from ovarectomy specimens in the laboratory: a promising ‘ex vivo’ method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe. J. Assist. Reprod. Genet. 2015; 32: 1221-31.
  12. Sauerbrun-Cutler M.T., Vega M., Keltz M., McGovern P.G. In vitro maturation and its role in clinical assisted reproductive technology. Obstet. Gynecol. Surv. 2015; 70: 45-57.
  13. Revel A., Koler M., Simon A., Lewin A., Laufer N., Safran A. Oocyte collection during cryopreservation of the ovarian cortex. Fertil. Steril. 2003;79(5):1237-9.
  14. Friedler S., Koc O., Gidoni Y., Raziel A., Ron-El R. Ovarian response to stimulation for fertility preservation in women with malignant disease: a systematic review and meta-analysis. Fertil. Steril. 2012; 97(1): 125-33.
  15. Sanchez F., Smitz J. Molecular control of oogenesis. Biochim. Biophys. Acta. 2012; 1822(12): 1896-912.
  16. Zhang Y., Wang H., Liu W., Yang Y., Wang X., Zhang Z., Guo Q., Wang C., Xia G. Natriuretic peptides improve the developmental competence of in vitro cultured porcine oocytes. Reprod. Biol. Endocrinol. 2017; 15(1): 41.
  17. Franciosi F., Coticchio G., Lodde V., Tessaro I., Modina S.C., Fadini R., Dal Canto M., Renzini M.M., Albertini D.F., Luciano A.M. Natriuretic peptide precursor C. delays meiotic resumption and sustains gap junction-mediated communication in bovine cumulus-enclosed oocytes. Biol. Reprod. 2014; 91(3): 61.
  18. Sanchez F., Lolicato F., Romero S., De Vos M., Van Ranst H., Verheyen G., Anckaert E., Smitz J.E.J. An improved IVM method for cumulus-oocyte complexes from small follicles in polycystic ovary syndrome patients enhances oocyte competence and embryo yield. Hum. Reprod. 2017; 32(10): 2056-68.
  19. Albuz F.K., Sasseville M., Lane M., Armstrong D.T., Thompson J.G., Gilchrist R.B. Simulated physiological oocyte maturation (SPOM): a novel in vitro maturation system that substantially improves embryo yield and pregnancy outcomes. Hum. Reprod. 2010; 25(12): 2999-3011.
  20. Lierman S., Tilleman K., Braeckmans K., Peynshaert K., Weyers S., T’Sjoen G., De Sutter P. Fertility preservation for trans men: frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles. J. Assist. Reprod. Genet. 2017; 34(11): 1449-56.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2019 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies