Characteristics of the vaginal microbiota in pregnant women with preterm premature rupture of the membranes


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Abstract

Objective. To investigate the vaginal microbiota in women with preterm premature rupture of the membranes (PROM) at 22-36 weeks and 6 days’ gestation. Subjects and methods. Examinations were made in 150 women aged 18 to 40 years, who were divided into 3 groups: 1) 50 pregnant women with PROM; 2) 50 pregnant women with intact membranes, who delivered at 22-36 weeks and 6 days’ gestation; 3) 50pregnant women who delivered spontaneously at term (at > 37 weeks). The vaginal microbiota underwent comprehensive microbiological and molecular genetic studies. Microbial species identification was carried out by time-of-flight mass spectrometry. Results. Pregnant women with PROM were somatically burdened with frequent respiratory and odontogenic inflammatory diseases; this pregnancy had a complicated course: threatened miscarriage due to protraction retroamniotic hematoma, the undulatory pattern of bloody vaginal discharge, and isthmic-cervical insuff iciency that required surgical correction. Cesarean section was a statistically significantly more common childbirth delivery method in pregnant women. The women with PROM had a mixed, dysbiotic vaginal microflora. Unlike pregnant women who delivered at term, the women with PROM were deficient in lactobacillus and had larger relative number of opportunistic pathogens, mainly facultatively anaerobic bacteria. Conclusion. The vaginal microbiota composition suggests that that there may be a risk for PROM. Monitoring the vaginal microbiota composition during pregnancy is a key step for the development of prognostic, prophylactic, and therapeutic strategies.

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About the authors

Zulfiya S. Khodzhaeva

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: zkhodjaeva@mail.ru

Gulnara E. Guseinova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: marysca666@rambler.ru

Vera V. Muravyeva

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_muravieva@oparina4.ru

Andrew E. Donnikov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: donnikov@dna-technology.ru

Nataliia D. Mishina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: mis7ha@gmail.com

Tatyana V. Priputnevich

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: priputl@gmail.com

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