Modeling the inflammatory process in the reproductive organs of female mice of the C57BL/6 and I/ST inbred strains with aerosolized Mycobacterium tuberculosis


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Abstract

Aim. To develop a model of the inflammatory process induced by aerosolized Mycobacterium tuberculosis (MTB) in the reproductive organs of female mice with different genetically determined susceptibilities to tuberculosis infection. Material and methods. Female mice of I/St (susceptible) and C57BL/6 (resistant) inbred strains were infected with aerosolized MTB H37Rv. Then, the development of reproductive organs inflammatory process and the state of the vaginal microbiota were studied. Results. C57BL/6 mice were observed to develop a vaginal flora imbalance with a tendency to form moderate dysbiosis by day 3 of MTB infection. No signif icant changes were seen in vaginal microbiota of I/St mice regardless of the timing of MTB infection. Histological examination showed a gradual development of the inflammatory process in reproductive organs of both strains, which was more pronounced in I/St mice. Conclusion. Inflammatory process induced by aerosolized MTB gradually develops in the reproductive organs of female mice and is more pronounced in highly MTB susceptible I/St strain.

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About the authors

Svetlana I. Kayukova

Central Research Institute for Tuberculosis

Email: kajukovalnp@gmail.com
PhD, senior researcher

Andrey E. Donnikov

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology of Minzdrav of Russia

Email: donnikov@dna-technology.ru
PhD, head of laboratory of molecular genetic methods

Irina V. Bocharova

Central Research Institute for Tuberculosis

Email: 3595598@mail.ru
candidate of biological sciences, head of animal facility

Elena L. Tumanova

N.I. Pirogov Russian National Research Medical University

Email: elena07tumanova@yandex.ru
MD, Professor, head of Department of pathologic anatomy and clinical pathologic anatomy of the pediatric Department

Maxim V. Mnikhovich

Research Institute of Human Morphology

Email: mnichmaxim@yandex.ru
PhD, senior researcher

Boris V. Nikonenko

Central Research Institute for Tuberculosis

Email: boris.nikonenko52@gmail.com
MD, leading researcher

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