Implications of mitochondrial DNA copy number in cumulus cells in late reproductive-age women


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Abstract

Objective. To estimate mitochondrial DNA (mtDNA) copy number in cumulus cells (CC) in patients of late reproductive age in in-vitro fertilization programs and to analyze the relationship of the indicators under study with the parameters of oogenesis, embryogenesis, embryo ploidy, and treatment outcomes. Subjects and methods. A prospective study was conducted to determine mtDNA copy number in 454 CC from 67 patients aged 35-45years. The relative level of mtDNA was determined by real time polymerase chain reaction. Results. A cohort of patients with a mean age of 37.8years showed a statistically significant relationship between the mean level of mtDNA in CC and age (p = 0.008), as well as the level of anti-Mtillerian hormone (p = 0.003). There was no correlation between mtDNA copy number in CC and oocyte maturity, fertilization rate, morpho- logical quality, and blastocyst ploidy. There was a tendency to an increase in mtDNA levels in CC in the embryo implantation group versus the non-embryo implantation group (p = 0.08). Conclusion. The determination of mtDNA copy number in CC can become a reliable biomarker of reproductive system aging, takin into consideration that the age given in the passport does not always reflect the true fertility potential of women. Also, the estimation of the level of mtDNA in CC may become a possible predictor of embryo implantation potential. However, further randomized trials are needed.

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About the authors

A. I Korolkova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: zaikinaai@icloud.com
PhD student of the1-st gynecology department 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

N. G Mishieva

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nondoc555@mail.ru
MD, Senior researcher of 1-st gynecology department 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

B. A Martazanova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: bellamart88@mail.ru
Ph.D. of the1-st gynecology department 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

O. V Burmenskaya

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_bourmenskaya@oparina4.ru
MD, Researcher of Molecular-genetic Laboratory 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

M. A Veyukova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: veymary@gmail.com
PhD, embryologist of the 1-st gynecology department 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

A. N Ekimov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_ekimov@oparina4.ru
MD, Geneticist, Laboratory of Molecular Genetic Techniques 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

D. Yu Trofimov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: d_trofimov@oparina4.ru
MD, Head of the Laboratory of Molecular Genetic Techniques 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

A. N Abubakirov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: nondoc555@yahoo.com
PhD, Head of the 1-st gynecology department 117997 Moscow, ak. Oparinastreet, 4. Тel: +7(915)322-08-79

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