Еndometrial microbiota of women with chronic endometritis and idiopathic infertility


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Abstract

Objective. To assess the composition of the microorganisms of the uterine cavity of women with chronic endometritis (CE) and idiopathic infertility. Materials and methods. The study included 145 women ofreproductive age with idiopathic infertility. All the patients underwent hysteroscopic examination followed by biopsy histopathological evaluation for immunohistochemical (IHC) and microbiological («Femoflor 16» and «Femoflor Screen» test, DNA-technology, Moscow) studies. Results. According to the results of the IHC study, the CE was detected in 113 (77.9%) patients. The most common microorganisms identified in the uterine cavity were Lactobacilli (66.21%), as well as Staphylococcus (44.83%), Enterobacteriaceae family (22.1%), Ureaplasma spp. (19.3%), Streptococcus spp. (15.2%), Gardnerella vaginalis (11.03%), Atopobium vaginae (10.34%). The presence of Ureaplasma spp. in the uterine cavity significantly increasing the risk of developing CE by 4,5 times (RR=4.483; 95% CI 1.003-20.029). Presence of Atopobium vaginae and Staphylococcus spp. increases the risk of developing pronounced CE by 7 times (RR=6.959; 95% CI 0.856-56.602) and 2.5 times (OS=2.5; 95% CI 1.034-6.043), respectively. Conclusion. The risk of developing CE increases with the persistence of certain microorganisms in the uterine cavity.

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About the authors

N. I Tapilskaya

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; St. Petersburg State Pediatric Medical University Ministry of Healthcare of Russian Federation

Email: tapnatalia@yandex.ru
MD, PhD, DSci (Medicine), Professor, Leading research scientist. The Department of Reproduction, Professor of the Department of Obstetrics and Gynecology 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation; 2 Litovskaya str., Saint Petersburg, 194100, Russian Federation

O. V Budilovskaya

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; St. Petersburg State Pediatric Medical University Ministry of Healthcare of Russian Federation

Email: o.budilovskaya@gmail.com
Researcher of the department of the medical microbiology, Assistant of the Department of Clinical Laboratory Diagnostics 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation; 2 Litovskaya str., Saint Petersburg, 194100, Russian Federation

A. A. Krysanova

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; St. Petersburg State Pediatric Medical University Ministry of Healthcare of Russian Federation

Email: krusanova.anna@mail.ru
Researcher of the Department of Medical microbiology, Assistant of the Department of Clinical Laboratory Diagnostics 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation; 2 Litovskaya str., Saint Petersburg, 194100, Russian Federation

G. K.H Tolibova

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

Email: gulyatolibova@yandex.ru
MD, PhD, Head of Immunohistochemistry Laboratory 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation

A. A Kopylova

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

Email: iagmail@ott.ru
Postgraduate student of the Department of reproduction 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation

N. D Tsypurdeeva

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

Email: tsypurdeevan@mail.ru
PhD, Researcher of the Department of reproduction 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation

A. M Gzgzyan

Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; St. Petersburg State University

Email: iagmail@ott.ru
MD, PhD, DSci (Medicine), Head of the Department of reproduction, Professor of the Department of Obstetrics, Gynecology and Reproduction, Medical Faculty 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation; 7-9 Universitetskaya nab., Saint Petersburg, 199034, Russian Federation

A. M Savicheva

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; St. Petersburg State Pediatric Medical University Ministry of Healthcare of Russian Federation

Email: savitcheva@mail.ru
MD, PhD, Professor, Head of the Department of Medical microbiology, Head of the Department of Clinical Laboratory Diagnostics 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation; 2 Litovskaya str., Saint Petersburg, 194100, Russian Federation

I. Y.U Kogan

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott; Saint Petersburg State University, Medical Faculty

Email: ikogan@mail.ru
MD, PhD, DSci (Medicine), Professor, Corresponding Member, Professor of the Department of Obstetrics, Gynecology and Reproduction, Medical Faculty 3 Mendeleyevskaya Line, Saint Petersburg, 199034, Russian Federation; 7-9 Universitetskaya nab., Saint Petersburg, 199034, Russian Federation

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