Certain acute-phase proteins and cytokines in the serum and follicular liquid of infertile patients with grades 1-2 external genital endometriosis


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Abstract

Objective. To study the levels of certain acute-phase proteins (α2-macroglobulin (α2-MG), α- antitrypsin (α-AT)); lactoferrin (LF) and albumin (ALB), cytokines (tumor necrosis factor-α (TNF-a); interleukin-6 and 8 (IL-6 and IL-8), interferon-γ (IFN-γ), as well as total protein (TP) in the infertile patients’ serum and follicular fluid as possible diagnostic markers for grades 1-2 external genital endometriosis (EGE), as well as in the prognosis of in vitro fertilization (IVF). Subjects and methods. Examinations were made in 32 infertile patients with grades 1-2 EGE (a study group) and in 37 healthy women with male factor infertility (a control group) who had undergone IVF treatment for fertility recovery in the Medica-2 Clinic, “Mother and Child” Group of Companies, Novokuznetsk (Russia). The investigators measured the levels of α2-MG and α-АТ by quantitative rocket immunoelectrophoresis; LF and cytokines by enzyme immunoassay; ALB and TP by biochemical assays (based on bromocresol green and biuret reagents, respectively). The results obtained were statistically analyzed in a free software environment for statistical data processing using the R version 3.4. Results. The infertile patients with grades 1-2 EGE versus the women in the control group were noted to have elevated serum levels of acute-phase proteins (a2-MG, a-AT, and ALB), TP, and IFN-γ. The specific feature of the composition of follicular fluid in this patient group was an increase in the concentration of IFN-y and a decrease in LF and IL-8. The serum ALB concentration above 47.5 g/l on the day of follicle puncture in patients with grades 1-2 EGE is an unfavorable background for the occurrence of pregnancy and is associated with the negative outcome of the IVF program. Conclusion. The increased concentrations of acute-phase proteins and cytokines (α2-MG, α-АТ, and IFN-γ), and TP in the blood of infertile women with grades 1-2 EGE can serve as additional diagnostic markers of the disease. The serum albumin level above 47.5 g/l on the day of follicle puncture was associated with the negative outcome of the IVF program in 80% of patients with grades 1-2 EGE, which allows one to recommend that this protein be identified as an additional criterion for predicting the effectiveness of IVF programs in order to expand indications for cryopreservation or delayed embryo transfer in more favorable conditions.

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About the authors

Victoria V. Likhacheva

Novokuznetsk State Institute for Postgraduate Training of Physicians, Branch, Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia; "Mother and Child" Group of Companies, "Medica-2" Clinic of Women's Health and Human Reproduction

Email: viroli@mail.ru

Xenia V. Krasnopolskaya

Moscow Regional Research Institute of Obstetrics and Gynecology

Email: ksu0207@mail.ru

Lyudmila G. Bazhenova

Novokuznetsk State Institute for Postgraduate Training of Physicians, Branch, Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia

Email: l_bagenova@mail.ru

Raisa M. Zorina

Novokuznetsk State Institute for Postgraduate Training of Physicians, Branch, Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia

Email: macroglobulin@yandex.ru

Arkadiy G. Markdorf

"Mother and Child" Group of Companies, "Medica-2" Clinic of Women's Health and Human Reproduction

Email: markdorf@mail.ru

Lyudmila V. Renge

Novokuznetsk State Institute for Postgraduate Training of Physicians, Branch, Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia

Email: l.renge@mail.ru

Tat’yana V. Tretyakova

Novokuznetsk State Institute for Postgraduate Training of Physicians, Branch, Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia

Email: ptv@yandex.ru

Svetlana V. Shramko

Novokuznetsk State Institute for Postgraduate Training of Physicians, Branch, Russian Medical Academy of Continuing Professional Education, Ministry of Health of Russia

Email: shramko_08@mail.ru

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