Use of granulocyte colony-stimulating factor and platelet-rich plasma in patients with “thin” endometrium in frozen embryo transfer programs


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To compare the effectiveness of granulocyte colony-stimulating factor (G- CSF) and autologous platelet-rich plasma (PRP) in patients with “thin” endometrium in frozen embryo transfer programs. Materials and methods. We conducted a prospective cohort study, which included 58 patients with “thin” endometrium. The group with PRP included 37 patients, G-CSF group included 21 patients. All patients received hormone replacement therapy (HRT). Patients in PRP group in addition to HRT were given an intrauterine injection of autologous PRP on the 8-9th, 10-11th, and 12-13th days of the menstrual cycle; patients in G-CSF group in addition to HRT were given an intrauterine injection of recombinant G-CSF on the 5-6th and 12-13th days of the menstrual cycle. The primary outcome was an increase in endometrial thickness greater than 7 mm on the day of embryo transfer, the secondary outcome was pregnancy rates. Results. Endometrial thickness greater than 7 mm on the day of embryo transfer in PRP group was observed in 26 (70.27%) patients, in G-CSF group - in 13 (61.9%) patients, the differences were not statistically significant (p=0.515). The average endometrial thickness on the day of embryo transfer in PRP group was 7.79 (1.42) mm, in G-CSF group - 7.21 (1.42) mm, the difference was not statistically significant (p=0.146). In PRP group embryo transfer was performed in 31 (83.78%) patients, and in G-CSF group in 12 (57.14%) patients, the difference was statistically significant (p=0.026). Pregnancy occurred in 16 (51.61%) patients in PRP group and in 4 (33.33%) patients in G- CSFgroup; the difference was not statistically significant (p=0.282). Conclusion. In our study we did not find statistically significant differences in either an increase in endometrial thickness or in the pregnancy rates between the two groups. Subsequent studies should be conducted to identify differences in the effectiveness of these treatment methods.

Full Text

Restricted Access

About the authors

L. G Dzhincharadze

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: lanachka@list.ru
graduate student of 1st gynecology department 117997 Russia, Moscow, Ac. Oparina str. 4

A. N Abubakirov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nondoc555@yahoo.com
PhD, head of 1st gynecology department 117997 Russia, Moscow, Ac. Oparina str. 4

N. G Mishieva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nondoc555@mail.ru
PhD, Senior researcher of 1st gynecology department 117997 Russia, Moscow, Ac. Oparina str. 4

T. A Fedorova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: t_fyodorova@oparina4.ru
Phd, professor, Head of department of transfusiology and extracorporeal hemocorrection 117997 Russia, Moscow, Ac. Oparina str. 4

E. M Bakuridze

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: eteri.bakuridze@mail.ru
PhD, head of clinical work of department of transfusiology and extracorporeal hemocorrection 117997 Russia, Moscow, Ac. Oparina str. 4

O. A Bystrykh

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: O_bystrikh@oparina4.ru
PhD, head of department of transfusion immunology and the preparation of blood components 117997 Russia, Moscow, Ac. Oparina str. 4

References

  1. Khalifa E., Brzyski R.G., Oehninger S., Acosta A.A., Muasher S.J. Sonographic appearance of the endometrium: the predictive value for the outcome of in vitro fertilization in stimulated cycles. Hum. Reprod. 1992; 7(5): 677-80. https:// dx.doi.org/10.1093/oxfordjournals.humrep.a137718.
  2. Zhang X., Chen C.H., Confino E., Barnes R., Milad M., Kazer R.R. Increased endometrial thickness is associated with improved treatment outcome for selected patients undergoing in vitro fertilization-embryo transfer. Fertil. Steril. 2005; 83(2): 336-40. https://dx.doi.org/10.1016Zj.fertnstert.2004.09.020.
  3. McWilliams G.D., Frattarelli J.L. Changes in measured endometrial thickness predict in vitro fertilization success. Fertil. Steril 2007; 88: 74-81. https://dx.doi. org/10.1016/j.fertnstert.2006.11.089.
  4. Basir G.S., O W.S., So W.W., Ng E.H., Ho P.C. Evaluation of cycle-to-cycle variation of endometrial responsiveness using transvaginal sonography in women undergoing assisted reproduction. Ultrasound Obstet. Gynecol. 2002;19(5): 48489. https://dx.doi.org/10.1046/j.1469-0705.2002.00685.x.
  5. Куликова Г.В., Абдурахманова Н.Ф., Файзуллина Н.М., Асатурова А.В., Щеголев А.И., Зиганшина М.М., Долгушина Н.В. Рецептивность «тонкого» эндометрия у пациенток в программах вспомогательных репродуктивных технологий. Акушерство и гинекология. 2019; 10: 100-7.
  6. Yang J.H., Chen M.J., Che C.D., Chen S.U., Ho H.N., Yang Y.S. Optimal waiting period for subsequent fertility treatment after hysteroscopic various surgeries. Fertil. Steril. 2013; 99(7): 2092-6. https://dx.doi.org/10.1016/j. fertnstert.2013.01.137.
  7. Casper R.F It’s time to pay attention to the endometrium. Fertil. Steril. 2011; 96(3): 519-21. https://dx.doi.org/10.1016/j.fertnstert.2011.07.1096.
  8. Sher G, Fisch J.D. Vaginal sildenafil (Viagra): a preliminary report of a novel method to improve uterine artery blood flow and endometrial development in patients undergoing IVF. Hum. Reprod. 2000; 15(4): 806-9. https://dx.doi. org/10.1093/humrep/15.4.806.
  9. Barash A., Dekel N, Fieldust S., Segal I., Schechtman E., Granot I. Local injury to the endometrium doubles the incidence of successful pregnancies in patients undergoing in vitro fertilization. Fertil. Steril. 2003; 79(6): 1317-22. https:// dx.doi.org/10.1016/s0015-0282(03)00345-5.
  10. Thomas J., Liu F, Link D.C. Mechanisms of mobilization of hematopoietic progenitors with granulocyte colony-stimulating factor. Curr. Opin. Hematol. 2002; 9(3): 183-9. https://dx.doi.org/10.1097/00062752-200205000- 00002.
  11. Dale D.C., Cottle T.E., Fier C.J., Bolyard A.A., Bonilla M.A., Boxer L.A. et al. Severe chronic neutropenia: treatment and follow-up of patients in the Severe Chronic Neutropenia International Registry. Am. J. Hematol. 2003; 72: 82-93. https://dx.doi.org/10.1002/ajh.10255.
  12. Gomez Raposo C., Pinto Marin A., Gonzalez Baron M. Colony-stimulating factors: clinical evidence for treatment and prophylaxis of chemotherapyinduced febrile neutropenia. Clin. Transl. Oncol. 2006; 8(10): 729-34. https://dx.doi. org/10.1007/s12094-006-0119-4.
  13. Gleicher N., Vidali A., Bar ad D.H. Successful treatment of unresponsive thin endometrium. Fertil. Steril. 2011; 95(6): 2123. e13-7. https://dx.doi. org/10.1016/j.fertnstert.2011.01.143.
  14. Gleicher N., Kim A., Michaeli T., Lee H.J., Shohat-Tal A.,.LazzaroniE., BaradD.H. A pilot cohort study of granulocyte colony-stimulating factor in the treatment of unresponsive thin endometrium resistant to standard therapies. Hum. Reprod. 2013; 28(1): 172-7. https://dx.doi.org/10.1093/humrep/ des370.
  15. Kunicki M., Lukaszuk K., Liss J., Skowrohska P., Szczyptahska J. Granulocyte colony stimulating factor treatment of resistant thin endometrium in women with frozen-thawed blastocyst transfer. Syst. Biol. Reprod. Med. 2017; 63(1): 49-57. https://dx.doi.org/10.1080/19396368.2016.1251505.
  16. Xu B., Zhang Q., Hao J., Xu D., Li Y. Two protocols to treat thin endometrium with granulocyte colony-stimulating factor during frozen embryo transfer cycles. Reprod. Biomed. Online. 2015; 30(4): 349-58. https://dx.doi.org/10.1016/j. rbmo.2014.12.006.
  17. Barad D.H., Yu Y., Kushnir V.A., Shohat-Tal A., Lazzaroni E., Lee H.J., Gleicher N. A randomized clinical trial of endometrial perfusion with granulocyte colony-stimulating factor in in vitro fertilization cycles:impact on endometrial thickness and clinical pregnancy rates. Fertil. Steril. 2014; 101(3): 710-5. https:// dx.doi.org/10.1016/j.fertnstert.2013.12.016.
  18. Tehraninejad E., Davari Tanha F., Asadi E., Kamali K., Aziminikoo E., Rezayof E. G-CSF intrauterine for thin endometrium, and pregnancy outcome. J. Fam. Reprod. Health. 2015; 9(3): 107-12.
  19. Li Y., Pan P., Chen X., Li L., Li Y., Yang D. Granulocyte colony-stimulating factor administration for infertile women with thin endometrium in frozen embryo transfer program. Reprod. Sci. 2014 21(3): 381-5. https://dx.doi. org/10.1177/1933719113497286.
  20. Maryam Eftekhar, Mozhgan Sayadi, Farideh Arabjahvani. Transvaginal perfusion of G-CSF for infertile women with thin endometrium in frozen ET program: A nonrandomized clinical trial. Iran. J. Reprod. Med. 2014; 12(10): 661-6.
  21. Mishra V., Choudhary S., Sharma U., Aggarwal R., Agarwa R., Gandhi K., Goraniya N. Effects of Granulocyte Colony-Stimulating Factor (GCSF) on persistent thin endometrium in frozen embryo transfer (FET) cycles. J. Obstet. Gynecol. India. 2016; 66(Suppl. 1): S407-11. https://dx.doi.org/10.1007/ s13224-015-0775-9.
  22. Lee J.W., Kwon O.H., Kim T.K., Cho Y.K., Choi K.Y., Chung H.Y. et al. Plateletrich plasma: quantitative assessment of growth factor levels and comparative analysis of activated and inactivated groups. Arch. Plast. Surg. 2013; 40(5): 530-5. https://dx.doi.org/10.5999/aps.2013.40.5.530.
  23. Grageda E., Lozada J.L., Boyne P.J., Caplanis N., McMillan P.J. Bone formation in the maxillary sinus by using platelet-rich plasma: an experimental study in sheep. J. Oral Implantol. 2005; 31(1): 2-17. https://dx.doi.org/10.1563/0-692.1.
  24. Dhillon R.S., Schwarz E.M., Maloney M.D. Platelet-rich plasma therapy - future or trend? Arthritis Res. Ther. 2012; 14(4): 219. https://dx.doi.org/10.1186/ ar3914.
  25. Chang Y., Li J., Chen Y., Wei L., Yang X., Shi Y., Liang X. Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization. Int. J. Clin. Exp. Med. 2015; 8(1): 1286-90.
  26. Tandulwadkar S.R., Naralkar M.V., Surana A.D., Selvakarthick M., Kharat A.H. Autologous intrauterine platelet-rich plasma instillation for suboptimal endometrium in frozen embryo transfer cycles: A pilot study. J. Hum. Reprod. Sci. 2017; 10(3): 208-12. https://dx.doi.org/10.4103/jhrs.JHRS_28_17.
  27. Molina A., Sanchez J., Sanchez W., Vielma V. Platelet-rich plasma as an adjuvant in the endometrial preparation of patients with refractory endometrium. JBRA Assist. Reprod. 2018; 22(1): 42-8. https://dx.doi.org/10.5935/1518-0557.20180009.
  28. Eftekhar M., Neghab N., Naghshineh E., Khani P. Can autologous platelet rich plasma expand endometrial thickness and improve pregnancy rate during frozen-thawed embryo transfer cycle? A randomized clinical trial. Taiwan. J. Obstet. Gynecol. 2018; 57(6): 810-3. https://dx.doi.org/10.1016/j.tjog.2018. 10.007.
  29. Chang Y., Li J., Wei L.N., Pang J., Chen J., Liang X. Autologous platelet-rich plasma infusion improves clinical pregnancy rate in frozen embryo transfer cycles for women with thin endometrium. Medicine (Baltimore). 2019; 98(3): e14062. https://dx.doi.org/10.1097/MD.0000000000014062.
  30. Zadehmodarres S., Salehpour S., Saharkhiz N., Nazari L. Treatment of thin endometrium with autologous platelet-rich plasma: a pilot study. JBRA Assist. Reprod. 2017; 21(1): 54-6. https://dx.doi.org/10.5935/1518-0557. 20170013.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies