Diagnostic value of determining proinflammatory factors of mitochondrial origin in women with normal pregnancy, threatened and recurrent miscarriages


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Abstract

Objective. To determine the content of proinflammatory factors of mitochondrial origin (mtDAMPs), namely OPA1 and TFAM proteins, in peripheral blood microvesicles in dynamics in women with threatened miscarriage (TM), recurrent miscarriage (RM) and during normal pregnancy (NP). Materials and methods. A total of 89 pregnant women were enrolled in the study including 32 patients with RM, 29 patients with TM and 28 women with NP. Peripheral blood was collected from the 6th to 37th week of pregnancy at 3-4-week intervals. To determine the level of mtDAMPs, microvesicles were isolated from the obtained blood plasma and the content of mtDAMPs was analyzed using the Western blot method. The GraphPad Prism 8.0 program was used for statistical processing of the obtained data. Results. Maximum values of ORA1 and TFAM proteins were registered in patients with RM at 12 weeks, in patients with TM at 18 weeks gestation, and in women with NP at 25 weeks gestation. There was a sharp increase in mtDAMP level in patients with RM at the end of the first trimester which was accompanied by a decrease in the effectiveness of the system for eliminating damaged mitochondria in exogenous oxidative stress associated with inflammation. Conclusion. Different levels of mtDAMPs in the early stages of pregnancy reflect the various states of the developing placenta in normal and complicated pregnancies, and contribute to the development of placental insuff iciency in women with TM and RM.

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About the authors

Yulia S. Bulatova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: yu.bulatova@mail.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Nana K. Tetruashvili

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_tetruashvili@oparina4.ru
Doctor of Medicine, Head of the Department of Pregnancy Loss Prevention and Therapy

Asmik G. Mikaelyan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: mikaelyan_asmik@bk.ru
Graduate student, Department of Pregnancy Loss Prevention and Therapy

Maria V. Marey

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: m_marey@oparina4.ru
researcher at the Laboratory of mitochondrial medicine

Julia A. Sukhanova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: suhanova_julia@hotmail.com
researcher at the Laboratory of mitochondrial medicine

Mikhail Y. Vysokikh

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: m_vysokikh@oparina4.ru
PhD, the Head of mitochondrial medicine research group

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