Mitochondrial DNA copy number in sperm of hiv-infected men receiving antiretroviral therapy


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Abstract

Objective. To study mitochondrial DNA (mtDNA) content in sperm samples of HIV-infected men receiving antiretroviral therapy (ARVT). Materials and methods. This prospective clinical study included 190patients who presented for the assessment of fertility and achieving conception. The main group consisted of 94 HIV-infected men; the control group included 96 patients without HIV infection. The quantitative assessment of mtDNA deletions (mtDNAdel-), total mtDNA copy number (mtDNAtotal) and their ratio (mtDNAtotal / mtDNAdel-) was carried out in 58 and 96 samples, respectively, using real-time polymerase chain reaction (PCR) method. Results. The median age of HIV-infected men was 37(33; 39) years, duration of the disease was 5 (2; 11) years. The median duration of receiving ARVT was 2 (1; 5) years. The patients of groups were similar in mtDNAdel-(1.9 (0.9; 5.9) and 2.4 (1.9; 5.6); р=0.54), mtDNAtotal (5.4 (3.7; 9.8) and 5.5 (3.5; 8.2); р=0.62) and their ratio mtDNAtotal /mtDNAdel- (2.4(1.5; 3.9) and 1.9 (1.3; 3.0); р=0.09), respectively. In the group with severe sperm abnormality there was a negative correlation between ARVT duration and copy number of mtDNAtotal (r=-0.627; р=0.01), as well as between ARVT duration and mtDNAdel- level (r=-0.542; р=0.04). The pronounced negative correlation was revealed between CD4+ level and ratio mtDNAtotal / mtDNAdel- (r=-0.29; р=0.03). Conclusion. HIV-infected men did not show significant differences in sperm mtDNA copy number in comparison with the men of the control group. HIV-infected men with severe sperm abnormality had inverse relationship between the levels of mtDNAtotal, mtDNAdel- and ARVT duration; mtDNAdel- content was negatively correlated to the immune system state.

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About the authors

S. G Perminova

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: perisvet@list.ru
Cand. Med. Sci.; Leading Researcher, Gynecology Department One 4, Oparin St., Moscow 117997

E. V Mityurina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: mity-elena@yandex.ru
Cand. Med. Sci.; Researcher, Gynecology Department One 4, Oparin St., Moscow 117997

F. N Selimova

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: doc.fselimova@mail.ru
Postgraduate Student, Gynecology Department One 4, Oparin St., Moscow 117997

O. A Burmenskaya

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: o_bourmenskaya@oparina4.ru
BD; Head, Laboratory of Cancer Genetics 4, Oparin St., Moscow 117997

N. V Kozyrina

Central Research Institute of Epidemiology, Federal Service on Customers' Rights Protection and Human Well-being Surveillance

Email: nad-kozyrina@yandex.ru
Cand. Med. Sci., Senior Researcher 3a, Novogireevskaya St., Moscow 111123

A. V Kravchenko

Central Research Institute of Epidemiology, Federal Service on Customers' Rights Protection and Human Well-being Surveillance

Email: alexey-kravtchenko@yandex.ru
MD; Professor, Leading Researcher 3a, Novogireevskaya St., Moscow 111123

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