Survival and composition of the cellular component of breast milk under various conditions of its expression and storage


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To determine the composition and survival of the cellular component of breast milk under various conditions of its single and double pumping and storage. Materials and methods. Eighty-six breast milk samples were collected using a Symphony Medela single (n = 63) or double (n = 23) clinical breast pump. Each sample was divided into two halves and stored for 72 hours when it was cooled to 4°C or room temperature. The fractions of living cells were assessed at 4, 24, and 72 hours after breast milk expression. Flow cytofluorometry was used to determine the expression of specific surface and intracellular markers. Results. The total number of cells in breast milk did not depend on the method of its expression, gestational age, or number of fetuses; the median was about 80,000 cells per ml. The main milk cell populations were epithelial cells and white blood cells; vimentin-positive stromal cells were a minor population. The storage of breast milk, regardless of its expression method, led to a gradual decrease in the number of living cells due to the partial death of short-lived leukocyte subpopulations. After 4 hours, about a third of all cells remained alive; cooling the milk to +4°C could prolong the period of this level of cell survival. Moreover, this effect was more pronounced in the samples obtained using a double clinical breast pump. Conclusion. The total number of cells in breast milk does not depend on its expression method, gestational age, or number of fetuses. To preserve the cellular component of the expressed breast milk, the latter can be stored at room temperature for the first 4 hours when it was cooled to +4°C for 24 hours; in this case double pumping has some advantage over single pumping.

Full Text

Restricted Access

About the authors

Irina I. Ryumina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: i_ryumina@oparina4.ru

Timur Kh. Fatkhudinov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Research Institute of Human Morphology

Email: tfat@yandex.ru

Irina V. Arutyunyan

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Research Institute of Human Morphology

Email: labrosta@yandex.ru

Andrey V. Makarov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Research Institute of Human Morphology

Email: anvitmak@yandex.ru

Anastasia V. Lokhonina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Research Institute of Human Morphology

Email: nastya.serbsky@gmail.com

Marina V. Narogan

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia

Email: m_narogan@oparina4.ru

Kamila R. Sharipova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k.r.sharipovaa@gmail.com

Irina V. Orlovskaya

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: i_orlovskaya@oparina4.ru

Victor V. Zubkov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia

Email: v_zubkov@oparina4.ru

Elena N. Baibarina

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

References

  1. Hassiotou F., Twigger A., Hartmann P.E., Hodgetts S., Filgueira L. Neural differentiation of human breastmilk stem cells. Paper presented at: International Society for Research in Human Milk and Lactation; 2012; Trieste, Italy.
  2. Weiler I.J., Hickler W., Sprenger R. Demonstration that milk cells invade the suckling neonatal mouse. Am J. Reprod Immunol. 1983; 4(2): 95-8. doi: 10.1111/j.1600-0897.1983.tb00261.x
  3. Zhou L., Yoshimura Y., Huang Y., Suzuki R., Yokoyama M., Okabe M., Shimamura M. Two independent pathways of maternal cell transmission to offspring: through placenta during pregnancy and by breast-feeding after birth. Immunology. 2000; 101(4): 570-81. doi: 10.1046/j.1365-2567.2000.00144.x
  4. Hassiotou F., Hepworth A.R., Metzger P., Tat Lai C., Trengove N., Hartmann P.E., et al. Maternal and infant infections stimulate a rapid leukocyte response in breastmilk. Clin Transl Immunol. 2013; 2(4): e3. doi: 10.1038/cti.2013.1
  5. Twigger A.J., Hodgetts S., Filgueira L., Hartmann P.E., Hassiotou F. From breast milk to brains: the potential of stem cells in human milk. J. Hum Lact. 2013; 29(2): 136-9. doi: 10.1177/0890334413475528.
  6. Twigger A.J., Hepworth A.R., Lai C.T., Chetwynd E., Stuebe A.M., Blancafort P., et al. Gene expression in breastmilk cells is associated with maternal and infant characteristics. Sci Rep. 2015; 5: 12933. doi: 10.1038/srep12933.
  7. Sani M., Hosseini S.M., Salmannejad M., Aleahmad F., Ebrahimi S., Jahanshahi S., Talaei-Khozani T. Origins of the breast milk-derived cells; an endeavor to find the cell sources. Cell Biol Int. 2015; 39(5): 611-8. http://dx.doi.org/10.1002/ cbin.10432
  8. Meier P.P., Engstrom J.L., Janes J.E., Jegier B.J., Loera F. Breast pump suction patterns that mimic the human infant during breastfeeding: Greater milk output in less time spent pumping for breast pump-dependent mothers with premature infants. J. Perinatol. 2012; 32: 103-10. doi: 10.1038/jp.2011.64.
  9. Eglash A., Simon L.; Academy of Breastfeeding Medicine. ABM Clinical Protocol #8: Human Milk Storage Information for Home Use for Full-Term Infants, Revised 2017. Breastfeed Med. 2017; 12(7): 390-5. doi: 10.1089/ bfm.2017.29047.aje.
  10. Jones F. Best Practice for Expressing, Storing and Handling Human Milk in Hospitals, Homes and Child Care Settings, Second Edition. The Human Milk Banking Association of NorthAmerica, (HMBANA). 3rd edition, 2011.
  11. ВОЗ. Руководство по грудному вскармливанию. Хранение сцеженного молока, 1997.
  12. Родригес Х.М. Микробиота женского молока. Consilium medicum/ Педиатрия, 2016; 4: 35-40.
  13. Prime D.K., Garbin C.P., Hartmann P.E., Kent J.C. Simultaneous breast expression in breastfeeding women is more efficacious than sequential breast expression. Breastfeed Med. 2012; 7: 442-7. doi: 10.1089/bfm.2011.0139
  14. Jones E., King C., Spencer A. Feeding and Nutrition in the Preterm Infant. Edinburgh. New York: Elsevier Churchill Livingstone, 2005. 205 р.
  15. Slutzah M., Codipilly C.N., Potak D., et al. Refrigerator storage of expressed human milk in the neonatl intensive care unit. J. Pediatr. 2010; 156: 26-8. doi: 10.1016/j.jpeds.2009.07.023.
  16. СанПиН 2.1.3.2630-10. Санитарно-эпидемиологические требования к организациям, осуществляющим медицинскую деятельность.
  17. Fan Y., Chong Y.S., Choolani M.A., Cregan M.D., Chan J.K.Y. Unravelling the Mystery of Stem/Progenitor Cells in Human Breast Milk. PLoS ONE. 2010; 5(12): e14421. https://doaj.org/article/3c23575beeb14201af7df8e124
  18. Indumathi S., Dhanasekaran M., Rajkumar J.S., Sudarsanam D. Exploring the stem cell and non-stem cell constituents of human breast milk. Cytotechnology. 2013; 65(3): 385-93. doi: 10.1007/s00464-014-3917-8
  19. Hassiotou F., Hartmann P.E. At the Dawn of a New Discovery: The Potential of Breast Milk Stem Cells. Adv Nutr. 2014; 5(6): 770-8. doi: 10.3945/ an.114.006924.
  20. Pechoux C., Gudjonsson T., Ronnov-Jessen L., Bissell M.J., Petersen O.W. Human mammary luminal epithelial cells contain progenitors to myoepithelial cells. Dev Biol. 1999; 206: 88-99. doi: 10.1006/dbio.1998.9133
  21. Thomas E., Zeps N., Cregan M., Hartmann P., Martin T. 14-3-3a (sigma) regulates proliferation and differentiation of mmultipotentp63-positive cells isolated from human breastmilk. CellCycle. 2011; 10(2): 1-7. DOI: 10.4161/ cc.10.2.14470
  22. Hassiotou F., Geddes D.T., Hartmann P.E. Cells in human milk: state of the science. J. Hum Lact. 2013; 29(2): 171-82. doi: 10.1177/0890334413477242.
  23. Patki S., Kadam S., Chandra V., Bhonde R. Human breast milk is a rich source of multipotent mesenchymal stem cells. Human Cell. 2010; 23(2): 35-40. doi: 10.1111/j.1749-0774.2010.00083.x.
  24. Sani M., Hosseini S.M., Salmannejad M., Aleahmad F., Ebrahimi S., Jahanshahi S., Talaei-Khozani T. Origins of the breast milk-derived cells; an endeavor to find the cell sources. Cell Biol Int. 2015; 39(5): 611-8. http://dx.doi.org/10.1002/ cbin.10432
  25. Goldman A.S., Chheda S., Garofalo R. Spectrum of immunomodulating agents in human milk. Int J. Pediatr. 1997, 4: 491.
  26. Jin Y.Y., Wei Zhao, Cao R.M., Xi Wang, Wu S.M., Chen T.X. Characterization of immunocompetent cells in human milk of Han Chinese. J. Hum Lact. 2011; 27(2): 155-62. doi: 10.1177/0890334410392041.
  27. Hamprecht K., Maschmann J., Vochem M., Dietz K., Speer C.P., Jahn G. Epidemiology of transmission of cytomegalovirus from mother to preterm infant by breastfeeding. Lancet. 2001; 357: 513-8. doi: 10.1016/S0140-6736(00)04043-5

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2020 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies