Improvement of angiogenesis and reproductive outcomes in patients with chronic endometritis


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. To study the effect of exogenous cytokine therapy on angiogenesis and reproductive function in patients with chronic endometritis. Materials and methods. A total of 83patients with chronic endometritis (main group) were divided into subgroup I (n=43, 20 days of therapy) and subgroup II (n=40, 40 days of therapy). Aspiration biopsy was performed on day 20-24 of the cycle to obtain the endometrial samples. Markers of angiogenesis (VEGF, VEGFR1-2, CD34) were evaluated using immunohistochemistry (IHC). Results. After 20 days of therapy, there was a 1.3-fold increase in VEGFR-1 expression and a 1.1-fold increase in VEGFR-2 expression. After 40 days of therapy, the level of receptor expression increased by 1.4 times on average and reached the normal indicators. After 20 and 40 days of therapy, the number of CD34 increased by 1.6 and 1.9 times, respectively. The improvement of the parameters of tissue vascularization influenced the reproductive parameters. In subgroup I, the ratio of pregnancy rate to birth rate was 47.4%, and taking into account the number of progressing pregnancies, it was 63.2%. In subgroup II, the rate was 60.9%, taking into account completed pregnancies, and 86.9%, including deliveries expected in the near future. Conclusion. Exogenous cytokine therapy effectively promotes the restoration of angiogenic activity in the tissue and improves the indicators of reproductive function.

Full Text

Restricted Access

About the authors

Yulia E. Dobrokhotova

N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia

Email: pr.dobrohotova@mail.ru
Dr. Med. Sci., professor, Head of the Department of Obstetrics and Gynecology of Medical Faculty

Ekaterina I. Borovkova

N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia

Email: katyanikitina@mail.ru
Dr. Med. Sci., professor, Department of Obstetrics and Gynecology of Medical Faculty

Olga R. Nugumanova

City Clinical Hospital No. 40, Moscow

Email: nug-olga@yandex.ru
Head of the day hospital of the maternity hospital

References

  1. Доброхотова Ю.Э., Ганковская Л.В., Боровкова Е.И., Нугуманова О.Р. Экзогенная цитокинотерапия в лечении пациенток с хроническим эндометритом. Акушерство и гинекология. 2021; 2: 119-26. [Dobrokhotova Yu.E., Gankovskaya L.V., Borovkova E.I., Nugumanova O.R. Exogenous cytokine therapy in the treatment of patients with chronic endometritis. Obstetrics and Gynecology. 2021; 2: 119-26. (in Russian)]. https://dx.doi.org/10.18565/ aig.2021.2.119-126.
  2. Smith M., Hagerty K.A., Skipper B., Bocklage T. Chronic endometritis: a combined histopathologic and clinical review of cases from 2002 to 2007. Int. J. Gynecol. Pathol. 2010; 29(1): 44-50. https://dx.doi.org/ 10.1097/ PGP.0b013e3181ae81bb.
  3. Duvan C.I., Ozmen B., Satiroglu H., Atabekoglu C.S., Berker B. Does addition of low-dose aspirin and/ or steroid as a standard treatment in nonselected intracytoplasmic sperm injection cycles improve in vitro fertilization success? A randomized, prospective, placebo-controlled study. J. Assist. Reprod. Genet. 2006; 23(1): 15-21. https://dx.doi.org/10.1007/s10815-005-9003-3.
  4. Lambers M.J., Hoozemans D.A., Schats R., Homburg R., Lambalk C.B., Hompes P.G. Low-dose aspirin in non-tubal IVF patients with previous failed conception: a prospective randomized double-blind placebo-controlled trial. Fertil. Steril. 2009; 92(3): 923-9. https://dx.doi.org/10.1016/j. fertnstert.2008.07.1759.
  5. Polanski L.T., Barbosa M.A., Martins W.P., Baumgarten M.N., Campbell B., Brosens J. et al. Interventions to improve reproductive outcomes in women with elevated natural killer cells undergoing assisted reproduction techniques: a systematic review of literature. Hum. Reprod. 2014; 29(1): 65-75. https:// dx.doi.org/10.1093/humrep/det414.
  6. Ledee-Bataille N., Bonnet-Chea K., Hosny G., Dubanchet S., Frydman R., Chaouat G. et al. Role of the endometrial tripod interleukin-18, -15, and -12 in inadequate uterine receptivity in patients with a history of repeated in vitro fertilization-embryo transfer failure. Fertil. Steril. 2005; 83(3): 598-605. https:// dx.doi.org/10.1016/j.fertnstert.2004.11.021.
  7. Quenby S., Kalumbi C., Bates M., Farquharson R., Vince G. Prednisolone reduces preconceptual endometrial natural killer cells in women with recurrent miscarriage. Fertil. Steril. 2005; 84(4): 980-4. https://dx.doi.org/ 10.1016/j. fertnstert.2005.05.012.
  8. Siristatidis C., Vrachnis N., Vogiatzi P., Chrelias C., Quinteiro-Retamar A., Bettocchi S. et al. Potential pathophysiological mechanisms of the beneficial role of endometrial injury in in vitro fertilization outcome. Reprod. Sci. 2014; 21(8): 955-65. https://dx.doi.org/10.1177/1933719114525270.
  9. Kuroda K., Venkatakrishnan R., James S., Sucurovic S., Mulac-Jericevic B., Lucas E.S. et al. Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells. J. Clin. Endocrinol. Metab. 2013; 98(11): 4429-37. https://dx.doi.org/10.1210/jc.2013-1977.
  10. Boomsma C.M., Keay S.D., Macklon N.S. Peri-implantation glucocorticoid administration for assisted reproductive technology cycles. Cochrane Database Syst. Rev. 2012; (6): CD005996. https://dx.doi.org/10.1002/ 14651858.CD005996.pub3.
  11. Cha J., Vilella F., Dey S.K., Simon C. Molecular interplay in successful implantation. In: Sanders S., ed. Ten critical topics in reproductive medicine. Washington, DC: Science/AAAS; 2013: 44-8.
  12. O’Neill C. The potential roles for embryotrophic ligands in preimplantation embryo development. Hum. Reprod. Update. 2008; 14(3): 275-88. https:// dx.doi.org/10.1093/humupd/dmn002.
  13. Доброхотова Ю.Э., Ганковская Л.В., Бахарева И.В., Свитич О.А., Малушенко С.В., Магомедова А.М. Роль иммунологических механизмов в патогенезе невынашивания беременности. Акушерство и гинекология. 2016; 7: 5-10. [Dobrokhotova Yu.E., Gankovskaya L.V., Bakhareva I.V., Svitich O.A., Malushenko S.V., Magomedova A.M. Role of immune mechanisms in the pathogenesis of miscarriage. Obstetrics and Gynecology. 2016; 7: 5-10. (in Russian)]. https://dx.doi.org/10.18565/ aig.2016.7.5-10.
  14. Mcqueen D.B., Bernardi L.A., Stephenson M.D. Chronic endometritis in women with recurrent early pregnancy loss and/or fetal demise. Fertil. Steril. 2014; 101(4): 1026-30. https://dx.doi.org/10.1016/j.fertnstert.2013.12.031.
  15. Арутюнян И.В., Кананыхина Е.Ю., Макаров А.В. Роль рецепторов VEGF-A165 в ангиогенезе. Клеточная трансплантология и тканевая инженерия. 2013; 8(1): 12-8.
  16. Нефедова Н.А., Харлова О.А., Данилова Н.В., Мальков П.Г., Гайфуллин Н.М. Маркеры ангиогенеза при опухолевом росте. Архив патологии. 2016; 78(2): 55-62.
  17. Толибова Г.Х., Траль Т.Г., Айламазян Э.К., Коган И.Ю. Молекулярные механизмы циклической трансформации эндометрия. Журнал акушерства и женских болезней. 2019; 68 (1): 5-12. [Tolibova G.Kh., Tral T.G., Aylamazyan E.K., Kogan I.Yu. Molecular mechanisms of cyclic transformation of the endometrium. Journal of Obstetrics and Women's Diseases. 2019; 68 (1): 5-12. (in Russian)]. https://doi.org/10.17816/ JOWD6815-12.
  18. Шуршалина А.В. Хронический эндометрит как причина нарушений репродуктивной функции. Гинекология. 2012; 14(4): 16-8.
  19. Толибова Г. Х., Траль Т. Г., Клещёв М. А., Кветной И. М., Айламазян Э.К. Эндометриальная дисфункция: алгоритм гистологического и иммуноги-стохимического исследования. Журнал акушерства и женских болезней. 2015; 67 (4): 69-77.
  20. Mueller M.D., Lebovic D.I., Garrett E., Taylor R.N. Neutrophils infiltrating the endometrium express vascular endothelial growth factor: potential role in endometrial angiogenesis. Fertil. Steril. 2000; 74(1): 107-12. https:// dx.doi.org/10.1016/s0015-0282(00)00555-0.
  21. Sharkey A.M., Day K., McPherson A., Malik S., Licence D., Smith S.K. et al. Vascular endothelial growth factor expression in human endometrium is regulated by hypoxia. J. Clin. Endocrinol. Metab. 2000; 85(1): 402-9. https://dx.doi.org/10.1210/ jcem.85.1.6229.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2021 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies