Blood and peritoneal fluid levels of soluble immunoregulatory molecules in patients with endometriosis
- Authors: Vtorushina V.V.1, Korotkova T.D.1, Krechetova L.V.1, Inviyaeva E.V.1, Van'ko L.V.1, Adamyan L.V.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Issue: No 6 (2021)
- Pages: 85-94
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/249181
- DOI: https://doi.org/10.18565/aig.2021.6.85-94
- ID: 249181
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Abstract
Aim. To investigate blood and peritoneal fluid concentrations of soluble immunoregulatory molecules in patients with different stages of endometriosis.Materials and methods. The study included women with stage I-II (n=12) and III-IV (n=28) endometriosis and a control group (C) (n=15). Peripheral venous blood samples were drawn from the study subjects before surgery, and peritoneal fluid samples were obtained intraoperatively. The samples were tested for soluble immunoregulatory molecules (IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IFN-g, TNFα, MCP-1, MIP-1α, MIP-1β, IP-10, CSF, GM-CSF, PDGF-bb, R ANTES, Eotaxin, VEGF, FGF basic).Results. There were no differences in soluble protein concentrations both between the study groups and the control group. Compared with the control subjects, patients with endometriosis in both groups had significantly higher levels of IL-6, IL-8, IL-10, and a tendency to higher concentrations of chemokines MCP-1 and MIP-1β in the peritoneal fluid. The assessment of the diagnostic value of the determination of peritoneal fluid IL-6, IL-8, MIP-1 МС, and MCP-1 levels in different stages of endometriosis was carried out. The diagnostic value of elevated levels of peritoneal fluid MCP-1 and MIP-1β was higher in patients with > I-II stages of endometriosis and IL-6 and IL-8 in patients with stage III-IV endometriosis.Conclusion. Patients with endometriosis have increased concentrations of IL-6 and IL-10 in the peritoneal fluid. Patients with advanced stages of endometriosis have significant changes in ratios of pro-inflammatory IL-6 to anti-inflammatory IL-4 and pro-inflammatory IL-12p70 to anti-inflammatory IL-10. In patients with all stages of endometriosis, there was an increase in the levels of chemokines IL-8, MCP-1, and MIP-1β that activate inflammation and affect cell growth
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About the authors
Valentina V. Vtorushina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: v_vtorushina@oparina4.ru
Ph.D., Immunologist at the Laboratory of Clinical Immunology
Tatiana D. Korotkova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: t_korotkova@oparina4.ru
Obstetrician-Gynecologist, Ph.D. Student at the Department of Gynecology
Lubov V. Krechetova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: l_krechetova@oparina4.ru
Dr. Med. Sci., Head of the Laboratory of Clinical Immunology
Eugenia V. Inviyaeva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: e_inviyaeva@oparina4.ru
Ph.D. (bio.sci.), Senior Researcher at the Laboratory of Clinical Immunology
Ludmila V. Van'ko
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: lvanko@oparina4.ru
Dr. Med. Sci., Professor, Leading Researcher at the Laboratory of Clinical Immunology
Leyla V. Adamyan
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: adamyanleila@gmail.com
Dr. Med. Sci., Academician of the RAS, Professor, Head of the Department of Gynecology, Deputy Director for Science
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