Blood and peritoneal fluid effector and regulatory subpopulations of innate immunity cells in women with endometriosis


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Abstract

Aim. To compare blood and peritoneal fluid effector and regulatory subpopulations of innate immunity cells of women with different stages of endometriosis. Materials and methods. The study included women with stage I-II (n=12) and III-IV(n=28) endometriosis. Blood samples drawn from the study subjects before surgery and peritoneal fluid samples obtained intraoperatively were tested for mononuclear cells using flow cytometry. Results. The number of neutrophils and their ratio to lymphocytes were increased in the peripheral blood of women with endometriosis. There were no significant changes in the subpopulations of NK cells, neither between the groups of patients with endometriosis nor compared with the control group. However, patients in both groups had a pronounced decrease in the proportion of T-regulatory lymphocytes with CD4+CD25+CD127low/-phenotype (P=0.004), a decrease in the number of CD14highCD16low - classical (P=0.038) and an increase in CD14highCD16high - intermediate monocytes (P=0.028). The peritoneal fluid of patients with endometriosis was characterized by an increased proportion of cytotoxic CD3-CD56+CD16+- and regulatory CD56brigh'CD16dim-NK cells compared with control subjects. Conclusion. Changes in the ratio of blood neutrophils and lymphocytes and the ratio of monocyte subpopulations suggest the persistence of the systemic inflammatory process. The decrease in the proportion of T-regulatory cells in the peripheral blood and the increase in the proportion of cytotoxic and regulatory subpopulations of NK cells in the peritoneal fluid indicates dysregulation of immune response mechanisms contributing to the development of endometriosis.

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About the authors

Eugenia V. Inviyaeva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_inviyaeva@oparina4.ru
Ph.D. (bio.sci.), Senior Researcher at the Laboratory of Clinical Immunology

Tatiana D. Korotkova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: t_korotkova@oparina4.ru
Obstetrician-Gynecologist, Ph.D. Student at the Department of Gynecology

Valentina V. Vtorushina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_vtorushina@oparina4.ru
Ph.D., Immunologist at the Laboratory of Clinical Immunology

Lubov V. Krechetova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: l_krechetova@oparina4.ru
Dr. Med. Sci., Head of the Laboratory of Clinical Immunology

Ludmila V. Van'ko

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: lvanko@oparina4.ru
Dr. Med. Sci., Professor, Leading Researcher at the Laboratory of Clinical Immunology

Leyla V. Adamyan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: adamyanleila@gmail.com
Dr. Med. Sci., Academician of the RAS, Professor, Head of the Department of Gynecology, Deputy Director for Science

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