VAGINAL MICROBIOME IN PREGNANT WOMEN WITH PRETERM PRELABOR RUPTURE OF MEMBRANES AT 22-28 WEEKS' GESTATION


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Abstract

Aim. To investigate vaginal microbiome in pregnant women with preterm prelabor rupture of membranes (PPROM) before 28 weeks' gestation using metagenomic sequencing. Materials and methods. The study comprised 55 pregnant women, including patients with PPRPO before 28 weeks' gestation (group I) and women with a healthy pregnancy (group II). Results. Statistically significant between-group differences were found in the relative abundance of microorganisms for the vaginal microbiome regarding 13 genera of microorganisms including Lactobacillus, Pediococcus, Prevotella, Prevotella 6, Peptostreptococcus, Peptoniphilus, Anaerococcus, Fusobacterium, Parvimonas, Lawsonella, Sutterella, Mobiluncus, and Thermus. Also, there were statistically significant differences in detection rates of microorganisms' genera, including Pediococcus, Prevotella, Prevotella 6, Anaerococcus, Peptoniphilus, Parvimonas, Lawsonella, Sutterella, Mobiluncus, Thermus. Conclusion. The presence of bacteria of the genus Prevotella (especially Prevotella 6), Anaerococcus, Peptoniphilus, Parvimonas, Lawsonella, Sutterella, Mobiluncus, Thermus in the vaginal microbiome of pregnant women with PPRPO, and low relative abundance of bacteria of the genus Lactobacillus and Pediococcus during pregnancy are associated with preterm prelabor rupture of membranes before 28 weeks' gestation, which warrants further investigations.

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About the authors

Natalya B. KUZNETSOVA

Rostov State Medical University, Ministry of Health of Russia; Clinic of Professor Bushtyreva LLC

Email: lauranb@inbox.ru
Dr. Med. Sci., Professor at the Center for Simulation Training, RostSMU, Ministry of Health of Russia; Chief Physician of the Clinic of Professor Bushtyreva LLC. Rostov-on-Don, Russia

Irina O. BUSHTYREVA

Clinic of Professor Bushtyreva LLC

Email: kio4@mail.ru
Dr. Med. Sci., Professor Rostov-on-Don, Russia

Violetta S. DYBOVA

Rostov State Medical University, Ministry of Health of Russia; Perinatal Center

Email: viola-kovaleva@mail.ru
Ph.D. Student at RostSMU, Ministry of Health of Russia; obstetrician-gynecologist, Perinatal Center Rostov-on-Don, Russia

Viktoriya V. BARINOVA

Rostov State Medical University, Ministry of Health of Russia; Clinic of Professor Bushtyreva LLC

Email: victoria-barinova@yandex.ru
Ph.D., Teaching Assistant at the Department of Obstetrics and Gynecology No. 1 Rostov-on-Don, Russia

Dmitrii E. POLEV

Medical Genetics Center "Serbalab"

Email: vdudurich@cerbalab.ru
Ph.D. (Bio.Sci.in genetics), Chief Biologist St. Petersburg, Russia

Mikhail V. ASEEV

Medical Genetics Center "Serbalab"

Email: vdudurich@cerbalab.ru
General Director, Senior Researcher, Specialist in Laboratory Genetics St. Petersburg, Russia

Vasilisa V. DUDURICH

Medical Genetics Center "Serbalab"

Email: vdudurich@cerbalab.ru
Biologist-Geneticist, Director St. Petersburg, Russia

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