Comparative evaluation of different methods in the diagnosis of LSIL and HSIL


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Abstract

Objective. To conduct a comparative assessment of the informative value of routine cytology, liquid-based cytology, colposcopy, HPV testing after self-sampling and sampling by a health worker in the detection of LSIL, HSIL and cervical cancer. Materials and methods. The research enrolled 300patients (average age 36.7±8.2years). All women underwent testing including: routine cytology, liquid-based cytology, colposcopy, HPV testing (after self-sampling with Qvintip and sampling of the materials by a gynecologist). If cytology revealed precancerous lesions of the cervix, abnormal colposcopic patterns were detected, or high risk HPVDNA (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 genotypes) was found, women were offered targeted biopsy of the cervix. Results. Pathological changes were detected by any method in 39% of the patients (117/300). After a targeted biopsy of the cervix (n=82) LSIL, HSIL, CC were confirmed in 51.2 % (LSIL - 45.2%; HSIL - 50%; CC -4.7%). Sensitivity of methods in detection of LSIL were as follows: routine cytology and liquid-based cytology - 21%; HPV test after self-sampling or sampling by a medical worker - 57.9%; colposcopy - 57.9%. For detection of HSIL and cC sensitivity were 43.5, 34.8, 87, 91.3 and 78.3% respectively. Conclusion. The highest sensitivity for HSIL detection was found for hr-HPV testing (87% in case of sampling by a medical worker, 91.3% using the Qvintip device), the highest specificity was noted for cytological methods: 87.5% for routine cytology and 100% for liquid-based cytology.

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About the authors

Natalia V. Artymuk

Kemerovo State Medical University

Email: artymuk@gmail.com
MD, PhD, Professor, the Head of Obstetrics and Gynecology Department named after Professor G.A. Ushakova

Kristina V. Marochko

Kemerovo State Medical University

Email: marochkokv@mail.ru
PhD, assistant of Obstetrics and Gynecology Department named after Professor G.A. Ushakova

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