Influence of heavy metals on the effectiveness of assisted reproductive technologies depending on the polymorphism of genes of the detoxification system


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Abstract

Aim. To analyze the relationship between the levels of heavy metals (cadmium, mercury, lead) in the blood, polymorphism of genes of the biotransformation system of xenobiotics and the outcomes of assisted reproductive technologies (ART) in infertile women. Materials and methods. 300 married couples underwent infertility treatment using ART. The levels of mercury, cadmium and lead in the blood of patients were detected using inductively coupled plasma mass spectrometry. The allelic variants of genes of the detoxification system were determined by real-time polymerase chain reaction with melting curve analysis. Results. The blood levels of lead and mercury were significantly lower in patients with the absence of T allele of CYP1A1 gene and in patients with G allele of GSTP1 gene. Mercury level was also low in the absence of deletion of GSTT1 gene. The blood level of cadmium was not associated with genes polymorphism of xenobiotic biotransformation. The patients with absent T allele of CYP1A1 gene had low birth rate (р=0.0270), and low cumulative birth rate (р=0.0249): 51.6% in patients with T/Tgenotype, 38.1% in patients with T/C genotype and 20% in patients with С/С genotype. The combined index of CYP1A1*Pb in the blood significantly influenced fertilization of oocytes. Conclusion. The obtained data showed that polymorphic variants of genes of the detoxification system play an important role in the accumulation of heavy metals in the body of patients, as well as in reduced rate of oocyte fertilization in ART cycles.

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About the authors

Anastasia G. Syrkasheva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_syrkasheva@oparina4.ru
PhD, Senior Researcher of the IVF Department

Nataliya V. Dolgushina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_dolgushina@oparina4.ru
Dr. Med. Sci., Deputy Director - Head of the Department of Research Administration

Vladimir E. Frankevich

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_frankevich@oparina4.ru
PhD, Head of the Department of Systems Biology in Reproduction

Andrey E. Donnikov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, Head of the Laboratory of Molecular Genetic Methods

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