Comparative analysis of the gut microbiome’s profile of mothers and newborns with preterm and timely birth


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Abstract

Objective: To study the gut microbiota profile in women with preterm and term infants using next-generation sequencing (NGS). Materials and methods: A prospective cohort study of 30 mother-infant pairs was conducted. The women and newborns were divided into two groups depending on gestational age (GA) at birth; group I consisted of 15 mother-infant pairs with spontaneous preterm birth (PTB), the median GA was 32.8 (2.08) weeks, and group II included 15 mother-infant pairs with term delivery (TD) at 39.5 (0.98) weeks of gestation. Fecal samples were analyzed using NGS. Results: Meconium of newborns in both groups was not sterile; the dominant microorganisms were Acinetobacter and Cutibacterium (Propionibacteriaceae). Members of Bifidobacterium (Actinobacteria) were the most common microbes in maternal gut microbiota. Comparative analysis of the gut microbiota profile in mothers and infants in both groups did not show statistically significant differences (p>0.05). Conclusion: Meconium was not sterile; it was antenatally colonized through maternal-fetal transmission, and a unique gut microbiota was formed. It should be interpreted as a new important factor of perinatal programming.

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About the authors

Ksenia A. Gorina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: k_gorina@oparina4.ru
Junior Researcher, 1st Obstetric Department of Pregnancy Pathology

Zulfiya S. Khodzhaeva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: zkhodjaeva@mail.ru
Dr. Med. Sci., Professor, Deputy Director for Research of the Institute of Obstetrics

Jekaterina Shubina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Head of the Laboratory of Genomic Data Analysis

Margarita S. Rogacheva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Junior Researcher, Laboratory of Genomic Data Analysis

Kamilla T. Muminova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: k_muminova@oparina4.ru
Ph.D., Junior Researcher, 1st Obstetric Department of Pregnancy Pathology

Tatyana V. Priputnevich

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: priputl@gmail.com
Dr. Med. Sci., Head of the Departament of Microbiology, Clinical Pharmacology and Epidemiology

Elena I. Kutsar

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

resident doctor

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