Experimental technique for the transfer of pronuclear stage genetic material
- Authors: Sukhikh G.T.1, Kamaletdinov N.S.1, Nazarenko T.A.1, Martirosyan Y.O.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Issue: No 9 (2022)
- Pages: 114-121
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/249554
- DOI: https://doi.org/10.18565/aig.2022.9.114-121
- ID: 249554
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Abstract
Background: An increased proportion of late reproductive age women with aneuploid oocytes suggests the development of novel techniques capable of improving their quality. Some of them are based on the transfer of genetic material from the recipient to the donor cell. The article presents the preliminary results of an experimental study aimed at developing a technique for the transfer of the nucleus from the recipient to the donor cell. Objective: To identify the technical possibility of the transfer of the nuclei at late pronuclear stage. Materials and methods: The technique of nuclear transfer was practiced on abnormally fertilized oocytes (3PN) which were unsuitable for further use in the ART procedures and were to be disposed of. A total of 28 triploid zygotes were used and 14procedures of pronuclear transfer were carried out. Results: The transfer procedure resulted in successful fusion of transferred nuclei with enucleated cytoplast in 57% of cases, degeneration occurred in 43% of cases. Fragmentation with varying degrees (10-70%) was observed in all 8 successfully reconstructed zygotes, however, the percentage of compactification and blastulation was reduced; as a result, we obtained one embryo at the morula stage and one embryo at the blastocyst stage. The key point in the nuclear extraction process is the quality of the cytoplasm. The necessary material support as well as solutions and their concentrations were determined, the effectiveness of virus HVJ-E in the cell membrane fusion was found to be acceptable. Conclusion: This experiment has shown the possibility of transferring pronuclei from one cell to another. It is necessary to continue developing the technique for the transfer of genetic material and modify it; it is worth developing the technique for the transfer of the material at other stages (for example, at metaphase IIspindle stage).
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About the authors
Gennady T. Sukhikh
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: g_sukhikh@oparina4.ru
Dr. Med. Sci., Professor, Academician of the RAS, Director
Nail S. Kamaletdinov
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: sunsh86@mail.ru
Embryologist at the ist Gynecological Department
Tatiana A. Nazarenko
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: t_nazarenko@oparina4.ru
Dr. Med. Sci., Professor, Director of the Institute of Reproductive Medicine
Yana O. Martirosyan
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: marti-yana@yandex.ru
Researcher at the Scientific and Educational Center for ART with the Clinical Division named after F. Paulsen
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