Characteristics of the organic acid profile of amniotic and cervicovaginal fluids in pregnant women at high risk for spontaneous preterm birth
- Authors: Gorina K.A.1, Khodzhaeva Z.S.1, Chagovets V.V.1, Starodubtseva N.L.1, Frankevich V.E.1, Priputnevich T.V.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Issue: No 3 (2022)
- Pages: 39-48
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/274193
- DOI: https://doi.org/10.18565/aig.2022.3.39-48
- ID: 274193
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Abstract
Objective: To study the metabolomic profile of amniotic and cervicovaginal fluids and identify the potential predictors for spontaneous preterm birth in high-risk patients. Materials and methods: The prospective study included 46 pregnant women at high risk for preterm birth, the informed consent was obtained from all the patients. The patients were divided into two groups: group I consisted of 12 pregnant women who had preterm birth and group II included 34 pregnant women who had full-term delivery. Amniotic fluid sampling was performed using diagnostic transabdominal amniocentesis. An Agilent 1260 high performance liquid chromatography system was used for the analysis of the samples. Results: The amniotic fluid of patients with spontaneous preterm birth had the panels of the following organic acids: N-acetylaspartic acid, 2-hydroxy-3-methylpentanoic acid, 4-hydroxyphenylpyruvic acid; sensitivity 92% and specificity 38%. The cervicovaginal fluid had N-acetyl-tyrosine, 2-oxoadipic acid, 3-methylglutaconic acid, 4-hydroxyphenylpyruvic acid; sensitivity 92% and specificity 82%. Conclusion: The identification of the organic acid panel in the amniotic fluid invasively and in the cervicovaginal fluid noninvasively using high-performance liquid chromatography makes it possible to predict the likelihood of spontaneous preterm labor.
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About the authors
Ksenia A. Gorina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: k_gorina@oparina4.ru
Junior Researcher at the Department of Pregnancy Pathology
Zulfiya S. Khodzhaeva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: zkhodjaeva@mam.ru
Dr. Med. Sci., Professor, Deputy Director for Research of the Institute of Obstetrics
Vitaliy V. Chagovets
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: vvchagovets@gmail.com
PhD, Senior Researcher at the Laboratory of Proteomics and Metabolomics of Human Reproduction
Nataliia L. Starodubtseva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: n_starodubtseva@oparina4.com
PhD, Head of the Laboratory of Proteomics of Human Reproduction
Vladimir E. Frankevich
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: v_frankevich@oparina4.ru
PhD, Head of the Department of Systems Biology in Reproduction
Tatyana V. Priputnevich
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: priputl@gmail.com
Dr. Med. Sci., Head of the Departament of Microbiology and Clinical Pharmacology
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