Prooxidant and antioxidant content in capillary blood in preterm birth
- Authors: Kan N.E.1, Salpagarova Z.K.1, Tyutyunnik V.L.1, Shchipitsyna V.S.1, Krasnyi A.M.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology Ministry of Health of the Russian Federation
- Issue: No 1 (2022)
- Pages: 56-61
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/274242
- DOI: https://doi.org/10.18565/aig.2022.L56-61
- ID: 274242
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Abstract
Objective: To determine the content of prooxidants and antioxidants in the capillary blood obtained at the same gestation stage from pregnant women who had preterm birth and normal pregnancy. Materials and methods: The study included 47 pregnant women. The main group consisted of 31 patients who had preterm birth and control group included 16 women with a normal pregnancy. The peripheral blood samples were obtained from the patients of both groups at the same gestation stage. The level of reactive oxygen species and antioxidant protection was determined by means of a FORM 3000 device using FORT and FORT kits (Callegari, Italy). The capillary blood was obtained and heparin was used as an anticoagulant. Statistical analysis and plotting were performed using Attestat (Russia), Statistica 10, and OriginPro 8.5 (USA) software. Results: There was an increase in the level of reactive oxygen species (ROS) in the main group in comparison with the control group. Their content in patients with preterm birth and normal pregnancy was 2.51 (2.06; 3.03) mmol/L and 1.22 (1.22; 1.66) mmol/L (p=0.01), respectively. The area under the ROC-curve was 0.90. The content of antioxidants in capillary blood was increased in the control group, namely 1.5 (1.03;2.31) mmol/L; their content was 0.97 (0.35;1.195) mmol/L in women with preterm birth. The area under the ROC-curve was 0.77. The combined measurement of both parameters (reactive oxygen species and antioxidants) showed the greatest significance. The area under the ROC-curve was 0.93. Conclusion: Preterm birth is associated with a systemic inflammatory response syndrome which is known to be accompanied by the development of oxidative stress leading to increased processes of apoptosis and disorders both at the cellular and subcellular levels. It is possible to determine the level of proinflammatory response and predict the development of complications, as well as to evaluate the effectiveness of therapy aimed at prolonging pregnancy by studying the indicators of oxidative stress and antioxidants.
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About the authors
Natalia E. Kan
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology Ministry of Health of the Russian Federation
Email: kan-med@mailru
Professor, MD, PhD, Deputy Director for Science 117997, Russia, Moscow, Ac. Oparina str., 4
Zalina Kh. Salpagarova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology Ministry of Health of the Russian Federation
Email: z.salpagarova1990@yandex.ru
postgraduate student 117997, Russia, Moscow, Ac. Oparina str., 4
Victor L. Tyutyunnik
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology Ministry of Health of the Russian Federation
Email: tioutiounnik@mail.ru
Professor, MD, PhD; Leading Researcher of Research and Development Service 117997, Russia, Moscow, Ac. Oparina str., 4
Valeriya S. Shchipitsyna
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology Ministry of Health of the Russian Federation
Email: alexred@list.ru
Junior Researcher of the Cytology Laboratory 117997, Russia, Moscow, Ac. Oparina str., 4
Aleksey M. Krasnyi
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology Ministry of Health of the Russian Federation
Email: alexred@list.ru
PhD, Head of the Cytology Laboratory 117997, Russia, Moscow, Ac. Oparina str., 4
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