Amino acid profile of blood plasma and follicular fluid in women with infertility and diminished ovarian reserve
- Authors: Shevtsova M.A.1, Gavisova A.A.1, Krasnova N.A.1, Aksenenko A.A.1, Novoselova A.V.1, Khazzhar F.1, Chagovets V.V.1, Frankevich V.E.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
- Issue: No 2 (2024)
- Pages: 79-88
- Section: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/631357
- DOI: https://doi.org/10.18565/aig.2023.270
- ID: 631357
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Abstract
Objective: This study aimed to investigate the amino acid profiles of blood plasma and follicular fluid in women with infertility and diminished ovarian reserve.
Materials and methods: The study included 115 women aged 18–42 years with infertility, referred to as V.I. Kulakov NMRC for OG&P, Ministry of Health of Russia for infertility treatment using ART (IVF/ICSI) and meeting the inclusion criteria. The groups were stratified according to ovarian reserve as follows: group 1 – 50 patients with diminished ovarian reserve (AMH <1.2 ng/ml, AFC<5); group 2 – 65 patients with normal ovarian reserve (AMH ≥1.2 ng/ml, AFC≥5) and matched for age. Analysis of amino acid levels in the blood plasma and follicular fluid on the day of oocyte aspiration was conducted using liquid chromatography with mass spectrometric detection.
Results: Analysis of the amino acid profile revealed a statistically significant decrease in sarcosine and tryptophan in women with diminished ovarian reserve compared to women with normal ovarian reserve. Additionally, there was a statistically significant decrease in the concentrations of lysine, asparagine, methionine, phenylalanine, and tryptophan in the follicular fluid of women with diminished ovarian reserve. Correlation analysis showed a strong correlation between amino acid levels in the plasma and follicular fluid, as well as between amino acid levels in the plasma and follicular fluid. It also revealed a moderate positive correlation between antral follicle counts and indicators of oogenesis and early embryogenesis, and the levels of amino acids, specifically lysine and sarcosine in blood plasma and asparagine, tryptophan, and aminobutyric acid in follicular fluid.
Conclusion: This study found a significant reduction in amino acid levels in infertile women with diminished ovarian reserve, which may serve as a marker for assessing ovarian reserve and oocyte development potential to enhance outcomes in ART programs.
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About the authors
Marina A. Shevtsova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: marina_981995@mail.ru
post-graduate student
Russian Federation, 117997, Moscow, Ac. Oparina str., 4Alla A. Gavisova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_gavisova@oparina4.ru
Dr.Med. Sci., Head of the 1st Gynecology Department
Russian Federation, 117997, Moscow, Ac. Oparina str., 4Natalya A. Krasnova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: dr.krasnova@rambler.ru
ORCID iD: 0000-0001-8636-2560
PhD, Teaching Assistant at the Department of Obstetrics, Gynecology, Perinatology and Reproductology
Russian Federation, 117997, Moscow, Ac. Oparina str., 4Artem A. Aksenenko
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_axenenko@oparina4.ru
PhD, Gynecologist at the 1st Gynecology Department
Russian Federation, 117997, Moscow, Ac. Oparina str., 4Anastasia V. Novoselova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_novoselova@oparina4.ru
Researcher at the Laboratory of Metabolomics and Bioinformatics
Russian Federation, 117997, Moscow, Ac. Oparina str., 4Fadi Khazzhar
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_novoselova@oparina4.ru
PhD, Junior Researcher at the Laboratory of Metabolomics and Bioinformatics
Russian Federation, 117997, Moscow, Ac. Oparina str., 4Vitaliy V. Chagovets
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: vvchagovets@gmail.com
PhD (Physico-mathematical Sciences), Head of the Laboratory of Metabolomics and Bioinformatics
Russian Federation, 117997, Moscow, Ac. Oparina str., 4Vladimir E. Frankevich
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: v_vfrankevich@oparina4.ru
Dr.Sci. (Physico-mathematical Sciences), Deputy Director of the Institute of Translational Medicine
Russian Federation, 117997, Moscow, Ac. Oparina str., 4References
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