Damage-associated molecular patterns in patients with intramural uterine fibroids and infertility

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Abstract

Objective: To investigate the levels of damage-associated molecular patterns (DAMPs) in fertile and infertile patients with uterine fibroids (UF).

Materials and methods: This single-center prospective study included 90 women. The main group comprised 60 patients with intramural UF, with 30 infertile (study group) and 30 fertile patients (comparison group). The control group consisted of 30 healthy women without uterine fibroids. The serum levels of DAMPs, including tumor necrosis factor alpha (TNF-α), protein S100, interleukin-10, glutathione, uric acid, low-density lipoprotein cholesterol (LDL-C), and fibrinogen, were measured.

Results: The data obtained indicated a significant increase in glutathione, uric acid, and LDL-C in the presence of UF, regardless of fertility, compared to the control group. More pronounced changes in parameters (increased protein levels of S100, uric acid, and LDL-C, as well as decreased glutathione levels) were observed in the presence of intramural UF and infertility compared to fertile and healthy patients. The proposed DAMPs markers (glutathione, LDL-C, S100 protein, and uric acid) can be used as diagnostic and prognostic markers of infertility in the presence of intramural UF. When threshold values are reached (glutathione less than 413 µmol/l; S100 protein more than 0.172 µg/l; uric acid more than 280.5 µmol/l; LDL-C more than 3.78 mmol/l), the relative risk of infertility increases.

Conclusion: This study significantly enhances our understanding of the pathogenesis of infertility in UF and confirms the presence of systemic metabolic disorders (oxidative-inflammatory stress) as a cofactor of infertility.

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About the authors

Ekaterina D. Dubinskaya

Patrice Lumumba Peoples' Friendship University of Russia

Author for correspondence.
Email: dubinskaya@yandex.ru
ORCID iD: 0000-0002-8311-0381

Dr. Med. Sci., Professor at the Department of Obstetrics, Gynecology and Perinatology

Russian Federation, Moscow

Svetlana N. Kolesnikova

Reaviz Moscow Medical University

Email: ksnmed@mail.ru
ORCID iD: 0000-0001-9575-0274

PhD, Associate Professor at the Department of Obstetrics, Gynecology and Pediatrics

Russian Federation, Moscow

Alexander S. Gasparov

Patrice Lumumba Peoples' Friendship University of Russia

Email: 13513522@mail.ru
ORCID iD: 0000-0001-6301-1880

Dr. Med. Sci., Professor, Professor at the Department of Obstetrics, Gynecology and Perinatology

Russian Federation, Moscow

Elizaveta V. Alyoshkina

Patrice Lumumba Peoples' Friendship University of Russia

Email: alyoshkina.ev@yandex.ru
ORCID iD: 0000-0001-5339-1285

Teaching Assistant at the Department of Obstetrics, Gynecology and Reproductive Medicine, Faculty of Postgraduate Education

Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Correlation of damage-associated molecular patterns (DAMPS) and infertility (p<0.001)

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3. Fig. 2. ROC-curve of the relationship between the presence of infertility and glutathione content in patients with intramural MM

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4. Fig. 3. ROC-curve of dependence of infertility and S100 protein content in patients with intramural MM

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5. Fig. 4. ROS curve of correlation between the presence of infertility and the content of LDL-C in patients with intramural MM

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6. Fig. 5. ROC-curve of the relationship between the presence of infertility and uric acid content in patients with intramural MM

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