Natural killer cell immunoglobulin-like receptors and their ligands in spouses and pregnancy outcomes of recurrent miscarriage treated with immunocytic therapy
- Authors: Krechetova L.V.1, Khoroshkeeva O.V.1, Tetruashvili N.K.1, Krechetov S.P.1, Jankevic T.E.2, Inviyaeva E.V.1, Trofimov D.Y.1, Sukhikh G.T.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- National Research Center – Institute of Immunology Federal Medical-Biological Agency of Russia
- Issue: No 11 (2024)
- Pages: 98-108
- Section: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/653433
- DOI: https://doi.org/10.18565/aig.2024.261
- ID: 653433
Cite item
Abstract
Relevance: Impaired fetoplacental blood flow is a primary cause of early pregnancy loss. The formation of the vascular bed during endometrial decidualization and control of trophoblast invasion involves natural killer (NK) cells, which express killer cell immunoglobulin-like receptors (KIR). The action of NK cells on target cells is determined by the interaction between the KIR and HLA class I molecules (HLA I). The characteristics of maternal and paternal KIR and HLA I molecules, which are KIR (KIR-L) ligands, as well as the NK cell-mediated allocompatibility reactions they induce have the potential to influence the efficacy of immunocytic therapy (ICT) during pregnancy.
Objective: To perform typing of classical HLA I and KIR genes in patients and their spouses among couples with idiopathic recurrent miscarriage (RM) to identify the characteristics of KIR genotypes and KIR-L representation in spouses with different pregnancy outcomes.
Materials and methods: This study included 39 married couples with RM. ICT was performed twice for each couple, both outside and during pregnancy. KIR and HLA I gene typing was conducted using maternal and paternal peripheral blood samples. Typing was performed using high-throughput NGS sequencing.
Results: Typing of KIR genes and classical HLA I genes in patients from married couples with idiopathic RM and treatment using ICT did not reveal any specific characteristics of KIR and KIR-L in spouses. Additionally, no association was found between the characteristics of KIR and KIR-L in spouses and pregnancy outcomes that occurred after ICT. These results do not provide sufficient evidence to suggest that KIR genotypes and classical HLA I in spouses are prerequisites for mother-fetus alloincompatibility in RM. Furthermore, the data did not reveal any combinations of KIR and KIR-L in spouses that affect the effectiveness of treatment involving ICT.
Conclusion: The lack of connection between the results of RM treatment involving ICT and the characteristics of the KIR genotypes and classical HLA I of spouses emphasizes the need for further investigation into the immunological processes involved in allogeneic mother-fetus interaction during pregnancy and maternal and paternal ICT. One promising area for further research is the study of KIR gene expression in NK cells of women with normal and pathological pregnancies.
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About the authors
Lyubov V. Krechetova
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Author for correspondence.
Email: l_krechetova@oparina4.ru
ORCID iD: 0000-0001-5023-3476
Dr. Med. Sci., Head of the Laboratory of Clinical Immunology
Russian Federation, 117997, Moscow, Oparin str., 4
Olga V. Khoroshkeeva
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: o_khoroshkeeva@oparina4.ru
ORCID iD: 0000-0002-5153-5422
Obstetrician-Gynecologist at the Department of Pregnancy Disorders (Miscarriage Treatment and Prevention Unit)
Russian Federation, 117997, Moscow, Oparin str., 4Nana K. Tetruashvili
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: n_tetruashvili@oparina4.ru
ORCID iD: 0000-0002-9201-2281
Dr. Med. Sci., Head of the Department of Pregnancy Disorders (Miscarriage Treatment and Prevention Unit)
Russian Federation, 117997, Moscow, Oparin str., 4Sergey P. Krechetov
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: s_krechetov@oparina4.ru
ORCID iD: 0000-0003-2861-6010
PhD, Leading Researcher at the Laboratory of Clinical Immunology
Russian Federation, 117997, Moscow, Oparin str., 4Tatjana E. Jankevic
National Research Center – Institute of Immunology Federal Medical-Biological Agency of Russia
Email: tat-shapovalov@yandex.ru
ORCID iD: 0000-0002-0105-3812
PhD, Junior Researcher at the Human Histocompatibility Genetics Laboratory
Russian Federation, 115522, Moscow, Kashirskoye Shosse, 24Evgenya V. Inviyaeva
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: e_inviyaeva@oparina4.ru
ORCID iD: 0000-0001-9878-3637
PhD, Leading Researcher at the Laboratory of Clinical Immunology
Russian Federation, 117997, Moscow, Oparin str., 4Dmitry Yu. Trofimov
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: d_trofimov@oparina4.ru
ORCID iD: 0000-0002-1569-8486
Corresponding Member of the RAS, Dr. Bio. Sci., Director of the Institute of the Reproductive Genetics
Russian Federation, 117997, Moscow, Oparin str., 4Gennady T. Sukhikh
Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: g_sukhikh@oparina4.ru
Academician of the RAS, Dr. Med. Sci., Professor, Director
Russian Federation, 117997, Moscow, Oparin str., 4References
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