Lysol oxidase-like 1 (LOXL1) levels in vaginal secretions in women of reproductive age with pelvic organ prolapse and the normal position of pelvic organs

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective: To evaluate the potential of non-invasive method for determining LOXL1 levels for the diagnosis of pelvic organ prolapse (POP) in women of reproductive age.

Materials and methods: A total of 48 women with POP and 32 women who had no vaginal prolapse were examined. LOXL1 levels in vaginal secretions were determined using enzyme-linked immunosorbent assay.

Results: LOXL1 levels were significantly higher in women with vaginal prolapse and in women with hypertension (p<0.05). LOXL1 levels were lower in women with varicose veins of the lower extremities, hemorrhoids and nephroptosis.

Conclusion: The study evaluated the potential of quantitative measurement of LOXL1 in vaginal secretions in women of reproductive age, that can become an effective prospective method to predict the diagnosis of pelvic organ prolapse.

Full Text

Restricted Access

About the authors

Irina S. Ivanyuk

Altai State Medical University, Ministry of Health of Russia

Author for correspondence.
Email: Ivanukirina@yandex.ru
ORCID iD: 0000-0002-6895-7103

PhD Student, Department of Obstetrics and Gynecology

Russian Federation, Barnaul

Olga V. Remneva

Altai State Medical University, Ministry of Health of Russia

Email: rolmed@yandex.ru
ORCID iD: 0000-0002-5984-1109

Dr. Med. Sci., Professor, Head of the Department of Obstetrics and Gynecology

Russian Federation, Barnaul

Yuri V. Korenovsky

City Polyclinic No. 14

Email: timidin@gmail.com
ORCID iD: 0000-0002-4434-5217

PhD, Associate Professor

Russian Federation, Barnaul

Anzhelika I. Gal’chenko

Altai State Medical University, Ministry of Health of Russia

Email: Ivanukirina@yandex.ru
ORCID iD: 0000-0003-3013-7764

PhD, Associate Professor, Department of Obstetrics and Gynecology

Russian Federation, Barnaul

References

  1. Anglès-Acedo S., Ros-Cerro C., Espuña-Pons M., Valero-Fernandez E.M.; en nombre del GISPEM. Sexual activity and function of women with severe pelvic organ prolapse subjected to a classical vaginal surgery. A Multicentre study. Actas. Urol. Esp. (Engl. Ed.). 2019; 43(7): 389-95. https://dx.doi.org/10.1016/ j.acuro.2019.02.003
  2. Abebe S.A., Gashaw Z.M., Ayichew Z., Angaw D.A., Kindie E.A. Prevalence and associated factors of depression among women with advanced pelvic organ prolapse in Northwest Ethiopia: cross-sectional study. BMC Womens Health. 2024; 24(1): 313. https://dx.doi.org/10.1186/s12905-024-03162-4
  3. Handa V.L., Blomquist J.L., Roem J., Munoz A., Dietz H.P. Pelvic floor disorders after obstetric avulsion of the levator ani muscle. Female Pelvic Med. Reconstr. Surg. 2019; 25(1): 3-7. https://dx.doi.org/10.1097/SPV.0000000000000644
  4. Reimers C., Siafarikas F., Stær-Jensen J., Smastuen M.C., Bo K., Ellström Engh M. Risk factors for anatomic pelvic organ prolapse at 6 weeks postpartum: a prospective observational study. Int. Urogynecol. J. 2019; 30(3): 477-82. https://dx.doi.org/10.1007/s00192-018-3650-2
  5. Handa V.L., Blomquist J.L., Carroll M.K., Munoz A. Genital hiatus size and the development of prolapse among parous women. Female Pelvic. Med. Reconstr. Surg. 2021; 27(2): e448-e452. https://dx.doi.org/ 10.1097/SPV.0000000000000960
  6. Mendes L.C., Bezerra L.R.P.S., Bilhar A.P.M., Neto J.A.V., Vasconcelos CTM, Saboia D.M. et al. Symptomatic and anatomic improvement of pelvic organ prolapse in vaginal pessary users. Int. Urogynecol. J. 2021; 32(4): 1023-9. https://dx.doi.org/10.1007/s00192-020-04540-w
  7. Короткевич О.С., Эйзенах И.А., Мозес В.Г., Захаров И.С. Клиническая эффективность вагинального тренажера в лечении несостоятельности мышц тазового дна у женщин пожилого возраста. Фундаментальная и клиническая медицина. 2018; 3(4): 32-8. [Korotkevich O.S., Eizenakh I.A., Mozes V.G., Zakharov I.S. Clinical efficiency of vaginal training device in treatment of pelvic organ prolapse in elderly women. Fundamental and Clinical Medicine. 2018; 3(4): 32-8. (in Russian)]. https://dx.doi.org/10.23946/ 2500-0764-2018-3-4-32-38
  8. Vandendriessche D., Sussfeld J., Giraudet G., Lucot J.P., Behal H., Cosson M. Complications and reoperations after laparoscopic sacrocolpopexy with a mean follow-up of 4 years. Int. Urogynecol. 2017; 28(2): 231-9. https://dx.doi.org/10.1007/s00192-016-3093-6
  9. Черёмин М.М., Смольнова Т.Ю., Красный А.М., Чупрынин В.Д., Особенности генной экспрессии у пациенток с пролапсом гениталий. Акушерство и гинекология. 2024; 3: 50-6. [Cheremin M.M., Smolnova T.Yu., Krasnyi A.M., Chuprynin V.D. Gene expression features in patients with genital prolapse. Obstetrics and Gynecology. 2024; (3): 50-6. (in Russian)]. https:// dx.doi.org/10.18565/aig.2024.17
  10. Li Y., Zhang Q.Y., Sun B.F., Ma Y., Zhang Y., Wang M. et al. Single-cell transcriptome profiling of the vaginal wall in women with severe anterior vaginal prolapse. Nat. Commun. 2021; 12(1): 87. https://dx.doi.org/ 10.1038/s41467-020-20358-y
  11. Martinez-Gonzalez J., Varona S., Canes L., Galan M., Briones A.M., Cachofeiro V. et al. Emerging roles of lysyl oxidases in the cardiovascular system: new concepts and therapeutic challenges. Biomolecules. 2019; 9(10): 610. https://dx.doi.org/10.3390/biom9100610
  12. Greene A.G., Eivers S.B., Dervan E.W.J., O'Brien C.J., Wallace D.M. Lysyl oxidase like 1: biological roles and regulation. Exp. Eye Res. 2020; 193: 107975. https://dx.doi.org/10.1016/j.exer.2020.107975
  13. Li Y., Nie N., Gong L., Bao F., An C., Cai H. et al. Structural, functional and molecular pathogenesis of pelvic organ prolapse in patient and Loxl1 deficient mice. Aging (Albany NY). 2021; 13(24): 25886-902. https:// dx.doi.org/10.18632/aging.203777
  14. Li Y., Wu B., An C., Jiang D., Gong L., Liu Y. et al. Mass cytometry and transcriptomic profiling reveal body-wide pathology induced by Loxl1 deficiency. Cell. Prolif. 2021; 54(7): e13077. https://dx.doi.org/10.1111/ cpr.13077
  15. Камоева С.В., Савченко Т.Н., Абаева Х.А., Демура Т.А., Иванова А.В. Роль матриксных белков Fbln-5 и LOXL-1 в патогенезе пролапса тазовых органов. Российский вестник акушера-гинеколога. 2013; 13(3): 33-7. [Kamoeva S.V., Savchenko T.N., Abaeva Kh.A., Demura T.A., Ivanova A.V. Role of the matrix proteins Fbln-5 and LOXL-1 in the pathogenesis of pelvic organ prolapse. Russian Bulletin of Obstetrician-Gynecologist. 2013; 13(3): 33-7. (in Russian)].
  16. Гречканев Г.О., Аветисян Е.А., Старкина О.В., Бабин Ю.Ю., Никишов Н.Н., Аветисян С.М., Сучилин Д.Н., Белоглазов Д.К. Генетические предикторы пролапса тазовых органов у женщин: версии и контраверсии. Российский вестник акушера-гинеколога. 2022; 22(4): 55-66. [Grechkanev G.O., Avetisyan E.A., Starkina O.V., Babin Yu.Yu., Nikishov N.N., Avetisyan S.M., Suchilin D.N., Beloglazov V.K. Genetic predictors of pelvic organs prolapse in women: versions and contraversions. Russian Bulletin of Obstetrician-Gynecologist. 2022; 22(4): 55-66. (in Russian)]. https://dx.doi.org/10.17116/rosakush20222204155
  17. Wu X., Liu X., Li T. Potential molecular targets for intervention in pelvic organ prolapse. Front. Med. (Lausanne). 2023; 10: 1158907. https://dx.doi.org/10.3389/fmed.2023.1158907
  18. Токтар Л.Р., Оразов М.Р., Геворгян Д.А., Арютин Д.Г., Маркина Я. В. Достиева Ш.М., Маслюков И.А. Трансперинеальное ультразвуковое исследование в диагностике несостоятельности тазового дна. Акушерство и гинекология: новости, мнения, обучение. 2020; 8(3) Приложение: 75-9. [Toktar L.R., Orazov M.R., Gevorgyan D.A., Aryutin D.G., Markina Ya.V. Dostieva Sh.M., Maslyukov I.A. The role of transperineal ultrasound assessment in the diagnosis of pelvic floor disorder. Оbstetrics and Gynecology: News, Opinions, Training. 2020; 8(3) Suppl.: 75-9. (in Russian)]. https://dx.doi.org/10.24411/2303-9698-2020-13912
  19. Klutke J., Ji Q., Campeau J., Starcher B., Felix J.C., Stanczyk F.Z. et al. Decreased endopelvic fascia elastin content in uterine prolapse. Acta Obstet. Gynecol. Scand. 2008; 87(1): 111-5. https://dx.doi.org/10.1080/00016340701819247
  20. Jung H.J., Jeon M.J., Yim G.W., Kim S.K., Choi J.R., Bai S.W. Changes in expression of fibulin-5 and lysyl oxidase-like 1 associated with pelvic organ prolapse. Eur. J. Obstet. Gynecol. Reprod. Biol. 2009; 145(1): 117-22. https://dx.doi.org/10.1016/j.ejogrb.2009.03.026
  21. Garcia B., Arthur A., Patel B., Chang J., Chen D., Lane F. A non-invasive determination of LOXL1 and fibulin-5 levels in the vaginal secretions of women with and without pelvic organ prolapse. J. Med. Res Surg. 2021; 2(2): 10.52916/jmrs214042. https://dx.doi.org/10.52916/jmrs214042
  22. Jameson S.A., Swaminathan G., Dahal S., Couri B., Kuang M., Rietsch A. et al. Elastin homeostasis is altered with pelvic organ prolapse in cultures of vaginal cells from a lysyl oxidase-like 1 knockout mouse model. Physiol. Rep. 2020; 8(11): e14436. https://dx.doi.org/10.14814/phy2.14436
  23. Borazjani A., Couri B.M., Kuang M., Balog B.M., Damaser M.S. Role of lysyl oxidase like 1 in regulation of postpartum connective tissue metabolism in the mouse vagina. Biol. Reprod. 2019; 101(5): 916-27. https://dx.doi.org/10.1093/biolre/ioz148
  24. Schlötzer-Schrehardt U., Zenkel M. The role of lysyl oxidase-like 1 (LOXL1) in exfoliation syndrome and glaucoma. Exp. Eye Res. 2019; 189: 107818. https://dx.doi.org/10.1016/j.exer.2019.107818
  25. Martinez-Revelles S., Garcia-Redondo A.B., Avendano M.S., Varona S., Palao T., Orriols M. et al. Lysyl oxidase induces vascular oxidative stress and contributes to arterial stiffness and abnormal elastin structure in hypertension: role of p38MAPK. Antioxid. Redox. Signal. 2017; 27(7): 379-97. https:// dx.doi.org/10.1089/ars.2016.6642
  26. Pascual G., Mendieta C., Mecham R.P., Sommer P., Bellon J.M., Bujan J. Down-regulation of lysyl oxydase-like in aging and venous insufficiency. Histol. Histopathol. 2008; 23(2): 179-86. https://dx.doi.org/10.14670/HH-23.179
  27. Youn Y.J., Lee J. Chronic venous insufficiency and varicose veins of the lower extremities. Korean. J. Intern. Med. 2019; 34(2): 269-83. https://dx.doi.org/10.3904/kjim.2018.230
  28. Nikolenko V.N., Oganesyan M.V., Vovkogon A.D., Cao Y., Churganova A.A., Zolotareva M.A. et al. Morphological signs of connective tissue dysplasia as predictors of frequent post-exercise musculoskeletal disorders. BMC Musculoskelet. Disord. 2020; 21(1): 660. https://dx.doi.org/10.1186/ s12891-020-03698-0
  29. Морозова Т.И., Перегудова И.Г. Проявление мезенхимальной дисплазии соединительной ткани у женщин с менструальной дисфункцией. Бюллетень медицинской науки. 2019; 4(16): 26-8. [Morozova T.I., Peregudova I.G. Manifestation of mesenchymal connective tissue dysplasia in women with menstrual dysfunction. Bulletin of Medical Science. 2019; 4(16): 26-8. (in Russian)].
  30. Beyens A., Van Meensel K., Pottie L., De Rycke R., De Bruyne M., Baeke F. et al. Defining the clinical, molecular and ultrastructural characteristics in occipital horn syndrome: two new cases and review of the literature. Genes (Basel). 2019; 10(7): 528. https://dx.doi.org/10.3390/genes10070528

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2025 Bionika Media