Shi L., Ronfard V. Biochemical and biomechanical characterization of porcine small intestinal submucosa (SIS): a mini review. Int. J. Burns Trauma. 2013; 3(4): 173-9

Irina Vladimirovna Arutyunyan; Арутюнян Ирина Владимировна; Timur Khasyanovich Tenchurin; Тенчурин Тимур Хасянович; Timofey Evgenievich Grigoryev; Григорьев Тимофей Евгеньевич; Andrey Vitalevich Makarov; Макаров Андрей Витальевич; Alexey Alexandrovich Korshunov; Коршунов Алексей Александрович; Andrei Vladimirovich Elchaninov; Ельчанинов Андрей Владимирович; Yevgenia Yurievna Kananykhina; Кананыхина Евгения Юрьевна; Anastasia Vyacheslavovna Lokhonina; Лохонина Анастасия Вячеславовна; Olesya Alexandrovna Vasyukova; Васюкова Олеся Александровна; Elena Vitalevna Uvarova; Уварова Елена Витальевна; Vladimir Dmitrievich Chuprynin; Чупрынин Владимир Дмитриевич; Alexey Dmitrievich Shepelev; Шепелев Алексей Дмитриевич; Vissarion Georgievich Mamagulashvili; Мамагулашвили Виссарион Георгиевич; Sergey Nikolaevich Chvalun; Чвалун Сергей Николаевич; Timur Khaisamudinovich Fatkhudinov; Фатхудинов Тимур Хайсамудинович

doi:10.18565/aig.2018.5.70-77

Shi L., Ronfard V. Biochemical and biomechanical characterization of porcine small intestinal submucosa (SIS): a mini review. Int. J. Burns Trauma. 2013; 3(4): 173-9

Authors: Arutyunyan I.V.¹^,2, Tenchurin T.K.³, Grigoryev T.E.³, Makarov A.V.¹^,4, Korshunov A.A.¹, Elchaninov A.V.¹^,2, Kananykhina Y.Y.¹^,2, Lokhonina A.V.¹, Vasyukova O.A.¹^,2, Uvarova E.V.¹, Chuprynin V.D.¹, Shepelev A.D.³, Mamagulashvili V.G.³, Chvalun S.N.³, Fatkhudinov T.K.¹^,5
Affiliations:
1. National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia
2. Research Institute of Human Morphology
3. Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute
4. N.I. Pirogov Russian National Research Medical University
5. Peoples' Friendship University of Russia
Issue: No 5 (2018)
Pages: 70-77
Section: Articles
URL: https://journals.eco-vector.com/0300-9092/article/view/248193
DOI: https://doi.org/10.18565/aig.2018.5.70-77
ID: 248193

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Abstract

Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina. Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells. Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9±32.5 pm. Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition.

Keywords

vagina, tissue engineering, polycaprolactone matrix, electrospinning, vaginal epithelium, multipotent

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References

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Shi L., Ronfard V. Biochemical and biomechanical characterization of porcine small intestinal submucosa (SIS): a mini review. Int. J. Burns Trauma. 2013; 3(4): 173-9

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Abstract

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About the authors

References

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