Shi L., Ronfard V. Biochemical and biomechanical characterization of porcine small intestinal submucosa (SIS): a mini review. Int. J. Burns Trauma. 2013; 3(4): 173-9


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina. Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells. Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9±32.5 pm. Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Irina Arutyunyan

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; Research Institute of Human Morphology

Email: labrosta@yandex.ru
PhD, Researcher, Laboratory of Regenerative Medicine; researcher of the Laboratory of Growth and Development

Timur Tenchurin

Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute

Email: tenchurin.timur@mail.ru
Ph.D., Research Fellow, Laboratory of Polymer Materials

Timofey Grigoryev

Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute

Email: timgrigo@yandex.ru
Ph.D., Head of the Department of Nanobiomaterials and Structures

Andrey Makarov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; N.I. Pirogov Russian National Research Medical University

Email: anvitmak@yandex.ru
Candidate of Medical Sciences, Senior Researcher Laboratory of Regenerative Medicine; associate professor of the Department of Histology

Alexey Korshunov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: a_korshunov@oparina4.ru
obstetrician-gynecologist of the department of combined and complex methods of treatment of gynecological diseases

Andrei Elchaninov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; Research Institute of Human Morphology

Email: elchandrey@yandex.ru
Candidate of Medical Science, Senior Researcher Laboratory of Regenerative Medicine; Senior Researcher Laboratory of growth and development of Research Institute of Human Morphology

Yevgenia Kananykhina

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; Research Institute of Human Morphology

Email: e.kananykhina@gmail.com
m.n. Laboratory of Regenerative Medicine; graduate student

Anastasia Lokhonina

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: nastya.serbsky@gmail.com
m.n. Laboratory of Regenerative Medicine

Olesya Vasyukova

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; Research Institute of Human Morphology

Email: olesya.vasyukova.rnrmu@gmail.com
the executor of the Agreement with the Ministry of Education and Science of the Russian Federation; m.n. Laboratory of growth and development

Elena Uvarova

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: elena-uvarova@yandex.ru
MD, professor, head. 2-nd gynecological department (gynecology of children and adolescents); President of the Interregional Public Organization “ Association of Children and Adolescent Gynecologists”.

Vladimir Chuprynin

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia

Email: v_chuprinin@oparina4.ru
Ph.D., head. surgical department

Alexey Shepelev

Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute

Email: shepelev_alexey@mail.ru
Ph.D., engineer-researcher of the laboratory of biocompatible matrices

Vissarion Mamagulashvili

Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute

Email: beso52@mail.ru
Ph.D., engineer-researcher of the laboratory of biocompatible matrices

Sergey Chvalun

Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute

Email: s-chvalun@yandex.ru
Doctor of chemical sciences, professor, deputy director for scientific work

Timur Fatkhudinov

National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; Peoples' Friendship University of Russia

Email: tfat@yandex.ru
MD, Ph.D. Laboratory of Regenerative Medicine; Professor of the Department of Histology, Cytology and Embryology

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