Direct mass spectrometry approach for the express identification of tumor tissue from breast cancer patients


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Objective. The development of analytical approach based on direct mass spectrometry analysis for the rapid identification and differentiation between tumor and healthy breast tissue. Subjects and methods. The case-control study included 25patients with invasive breast cancer. Each sample of tumor and healthy tissue from each patient was divided into two parts. One part was used for pathomorphological examination. Another part was investigated by direct mass spectrometry analysis to obtain molecular profile. The mass spectrometric data were processed by the OPLS-DA multivariate analysis. Results. The normal and tumor breast tissues were differentiated by OPLS-DA analysis of the mass spectrometric data. Lipids of 4 different classes contributed in classification: diacylglycerol DG 34:1, monoacylglycerols MG 18:1, MG O-14:1, phosphatidylcholines LPC 16:0, PC 32:0, PC 32:1, PC 34:0, PC 34:1, PC 34:2, PC 36:3, PC 38:3, PC 38:4, sphingomyelin SM 32:1. Conclusions. Direct mass spectrometry tissue analysis allows differentiation between tumor and healthy breast tissues based on characteristic patterns of lipid ion signals in the mass spectrum. Besides their diagnostic value, these characteristic patterns can also be used for the mechanistic study of tumor pathogenesis.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Alisa Tokareva

Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russia; Moscow Institute of Physics and Technology

Email: alisa.tokareva@phystech.edu
student

Vitaliy Chagovets

Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russia

Email: vvchagovets@gmail.com
PhD, Senior Researcher of Proteomics of Human Reproduction

Wang Zhihao

Moscow Institute of Physics and Technology; East China University of Technology

Email: frankevich@rambler.ru

Valeriy Rodionov

Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russia

Email: V_Rodionov@oparina4.ru
MD, Head of the Department of Breast Pathology

Vlada Kometova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: vladastasiatema@mail.ru
PhD, senior researcher

Maria Rodionova

Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russia

Email: m_Rodionova@oparina4.ru
MD, oncologist of the Department of Breast Pathology

Alexey Kononikhin

Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russia; Moscow Institute of Physics and Technology

Email: konoleha@yandex.ru
PhD, Researcher of Proteomics of Human Reproduction

Nataliia Starodubtseva

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; Moscow Institute of Physics and Technology

Email: n_starodubtseva@oparina4.ru
PhD, Head of Laboratory of Proteomics of Human Reproduction

K. Chingin

East China University of Technology

Email: chingin.k@hotmail.com

Vladimir Frankevich

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_frankevich@oparina4.ru
PhD, Head of Department of Systems Biology in Reproduction

Chen Huanwen

East China University of Technology

Email: chingin.k@hotmail.com
professor

Gennadiy Sukhikh

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Academician of RAS, MD, PhD, Professor, Director

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