The effectiveness of frozenthawed embryo transfer in patients with different transcriptional gene signatures of endometrial receptivity
- Autores: Miroshkina M.I.1, Korneeva I.E.1, Burmenskaya O.V.1, Mishina N.D.1
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Afiliações:
- V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Edição: Nº 11 (2020)
- Páginas: 85-92
- Seção: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/249043
- DOI: https://doi.org/10.18565/aig.2020.11.85-92
- ID: 249043
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Resumo
Aim. To investigate the outcomes of frozen-thawed embryo transfer in patients with different mRNA expression of 15 endometrial genes during the window of implantation. Materials and methods. We examined 36 patients with tubal-peritoneal infertility who underwent pipelle endometrial biopsy in the setting of cyclic hormonal therapy on the 5th day of progesterone administration (P + 5) in the cycle before embryo transfer. Investigations included a histological and molecular genetic study of endometrial gene expression using RT-PCR. After frozen-thawed embryo transfer, the outcomes of ART were analyzed. Results. The molecular genetic characteristics of the window of implantation were determined in patients with different embryo transfer outcomes. Based on these findings, interim stages in the receptive endometrium development were identified. Conclusion. Analysis of the mRNA expression of genes LIF, GPX3, AQP3, NDRG1, GNLY, IMPA2, PAEP, IGFBP1, HABP2, DPP4, TAGLN, IL15, POSTN, HLA-DOB, and MSX1 allows determination of the most favorable day for embryo transfer.
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Sobre autores
Maria Miroshkina
V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: maria_mir18@mail.ru
Postgraduate Student at the F. Paulsen SEC for ART
Irina Korneeva
V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of RussiaDr. Med. Sci., Head of F. Paulsen SEC for ART
Olga Burmenskaya
V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: o_bourmenskaya@oparina4.ru
Dr. Biol. Sci., Head of the Laboratory of Oncological Genetics
Natalia Mishina
V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: mis7ha@gmail.com
Junior Researcher at the Laboratory of Genomic Data Analysis
Bibliografia
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