Cervical intraepithelial neoplasia: Analysis of mRNA profile in the practice of liquid-based cytology


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Subjects and methods. The mRNA expression level of 21 genes (Ki-67, STK-15, CCNB1, CCND1, MYC, MYBL2, P16INK4A, PTEN, BIRC5, BCL2, BAG1, TERT, NDRG1, ESR1, PGR, HER2, GRB7, MGB1, MMP11, CTSL2, and CD68) was determined by quantitative PCR in the material of preservative fluid collection vials after CellPrep PAP test in 59 patients who had been treated at the Russian Research Center of Roentgenology and Radiology in 2015-2016. The test of significance was the results of comparison with subsequent histological examination. Results. Discriminant analysis has established that a combined assessment of ESR1 and MYBL2 mRNA expression levels allows correct classification in 88.24% of cases of CIN2+ changes and in less than 84.0% of cases of histologically confirmed CIN1. The combined assessment of mRNA expression levels of 17 genes (ESR1, MYBL2, CD68, PTEN, CCND1, BCL2, HER2, MMP11, TERT, STK15, P16INK4A, BAG1, CTSL2, KI67, CCNB1, GRB7, and NDRG1) permits differentiation of CIN 2+ and CIN1 or less cases with an accuracy at 98.3%. The coincidence rate of the classification with the data of histological examination was 100.0% for the CIN2+ group and 96.0% for the histologically confirmed CIN1 or less group. Conclusion. It has been shown for the first time that the mRNA expression of a large group of genes can be analyzed by quantitative PCR in the material of CellPrep liquid-based cytology in the PAP test. Assessment of the expression of a panel of 21 genes, which complements the preoperative morphological diagnosis of precancerous processes and cancer of the cervix uteri in the material of liquid-based cytology, allows a high-precision differential diagnosis of 2 clinically important groups, such as the CIN 2+ and CIN1 or less ones (intact epithelium/benign tissue changes of the cervix).

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作者简介

Nadezhda Melnikova

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: n_melnikova@list.ru
Ph.D., Leading Researcher of the Laboratory of Immunology, oncocytology and cell therapy in oncology

Vladimir Bozhenko

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: vbojenko@mail.ru
MD, Head of the Department of Molecular Biology and Experimental Tumor Therapy

Irina Antonova

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: iran24@yandex.ru
MD, Head of the Laboratory of prevention, early diagnostics and combination treatment of cancer diseases

Nataliya Babaeva

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: natbabaeva@yandex.ru
MD, Leading researcher of Laboratory of prevention, early diagnostics and combined treatments of cancer diseases

Nataliya Yarovaya

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: natalya-yarovaya@yandex.ru
Ph.D., Head of the Laboratory of Cytology at the Pathology department

Nataliya Bolotina

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: soloma-natali@yandex.ru
Ph.D., Pathologist at the Pathology department

Margarita Zakharenko

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: zak-margarita@mail.ru
Junior Researcher of the Laboratory of Immunology, oncocytology and cell therapy in oncology

Anna Senchukova

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Email: asenchukova@gmail.com
Ph.D., Researcher of the Laboratory of Immunology, oncocytology and cell therapy in oncology

Nataliya Akopova

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

Ph.D., Obstetrician-gynecologist of the Department of Radiation and instrumental diagnostics of tumor diseases of the reproductive organs

Nataliya Aleksandrova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: alexandrova.ncagip@gmail.com
MD

Olga Burmenskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: bourmenska@mail.ru
MD, Senior Researcher of Laboratory molecular genetic factors

Levon Ashrafyan

Russian Research Center of Roentgenology and Radiology, Ministry of Health of Russia

MD, Professor, Head of the Department of Early carcinogenesis, prevention, diagnostics and comprehensive treatment of oncological diseases of female reproductive organs

参考

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