Search for personalized implantation window markers in women in an in vitro fertilization program by identifying the transcriptional profile of genes


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Objective. To enhance the efficiency of determining the endometrial functional status during the implantation window based on the integrated assessment of gene transcripts as markers of endometrial readiness for embryo implantation. Subjects and methods. The investigation enrolled 71 women with realized reproductive function without endometrial disease; of them 47 patients did this after in vitro fertilization and embryo transfer. During the expected implantation window, pipelle endometrial biopsy in a natural cycle and histological examination were performed and the transcriptional profile of the GPX3, PAEP, DPP4, TAGLN, HABP2, IMPA2, AQP3, HLA-DOB, MSX1, and POSTN genes was determined by real-time quantitative reverse transcription polymerase chain reaction. Results. There were differences in the level of mRNA expression of all the examined genes in the receptive endometrium versus prereceptive one, which could classify two endometrial functional states. An integrated assessment of the mRNA expression of 4 genes (PAEP, DPP4, MSX1 and HLA-DOB) made it possible to determine endometrial receptivity. This was consistent with histological findings of the stage of endometrial maturation in 83% of cases. Conclusion. A model has been proposed to determine a personalized implantation window, which can be further used in assisted reproductive technology programs.

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作者简介

Olga Burmenskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_bourmenskaya@oparina4.ru
Ph.D., D.Sc., Senior Researsher of molecular-genetics Department

Vladimir Bozhenko

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: vbojenko@mail.ru
Ph.D., M.D., Professor, Consultant

Veronika Smolnikova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_smolnikova@oparina4.ru
M.D., Ph.D., Leading Researcher of the Department of assistive reproductive technology in the treatment of infertility

Elena Kalinina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
M.D., Ph.D., Head of the Department of assistive reproductive technology in the treatment of infertility

Irina Korneeva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: i_korneeva@oparina4.ru
M.D., Ph.D., Leading Researcher of the 1 gynecology department

Elena Mezhevitinova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_mezhevitinova@oparina4.ru
M.D., Ph.D., Leading Researcher of the scientific-patient Department

Andrey Donnikov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
Ph.D., senior staff scientist of molecular-genetics Department

Ekaterina Beik

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_beyk@oparina4.ru
graduate student

Kemalya Nabieva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: kemalya-n@yandex.ru
graduate student

Vladimir Naumov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: v_naumov@oparina4.ru
Scientific Researsher of laboratory bioinformatics

Pavel Borovikov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: p_borovikov@oparina4.ru
Head of laboratory bioinformatics

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