Optimization of art programs in women of late reproductive age by co-cultivation of oocytes with donor extracellular vesicles from follicular fluid

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Background: The effectiveness of infertility treatment using assisted reproductive technologies (ART) decreases with the age of the woman due to the aging of the germ cells. It has been previously shown that co-cultivation of embryos with autologous extracellular vesicles from follicular fluid (EVFF) improves the embryological stage of treatment. However, in women of late reproductive age, biological fluids senesce and lose their effectiveness. Therefore, a hypothesis about the use of donor EVFF to improve the results of ART has been proposed.

Objective: To evaluate the effectiveness of donor EVFF in co-cultivation of embryos of women over 40 years.

Materials and methods: The study was conducted among 79 married infertile couples. Inclusion criteria: the woman's age > 40 years, normal karyotype both in a woman and a man. The patients were divided into two groups: the main group (n=40) with co-cultivation of oocytes with donor EVFF and the comparison group (n=39) with standard cultivation. Stimulation of ovarian function was performed according to the protocol with gonadotropin-releasing hormone antagonists. Transvaginal puncture was done under anesthesia. In group 1, donor EVFF were added to fertilized oocytes. The next day, they were transferred to fresh culture medium. The main evaluation parameters included: fertilization rate, blastocyst formation, and clinical pregnancy.

Results: In the group with donor EVFF, the fertilization rate was higher (84.8% vs. 70%, p=0.008), the proportion of embryo transfer cancellations was lower (22.5% vs. 46.1%, p=0.021). The frequency of pregnancy and genetically normal embryos did not statistically differ.

Conclusion: Donor EVFF improve embryological parameters but do not affect clinical outcomes in women of late reproductive age.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Z. Akhmedova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Хат алмасуға жауапты Автор.
Email: zyuka-1997@mail.ru
ORCID iD: 0000-0002-4483-8820

PhD student at the Prof. B.V. Leonov Department for Assisted Technologies in Infertility Treatment

Ресей, Moscow

A. Sysoeva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: sysoeva.a.p@gmail.com
ORCID iD: 0000-0002-6502-4498

PhD, Clinical Embryologist at the Prof. B.V. Leonov Department for Assisted Technologies in Infertility Treatment

Ресей, Moscow

M. Gavrilov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: maxgavr67@gmail.com
ORCID iD: 0000-0001-6189-0287

Junior Researcher at the Prof. B.V. Leonov Department of Assistive Technologies in Infertility Treatment

Ресей, Moscow

B. Zingerenko

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: b_zingerenko@oparina4.ru
ORCID iD: 0000-0002-8784-5502

Junior Researcher at the Prof. B.V. Leonov Department of Assistive Technologies in Infertility Treatment

Ресей, Moscow

Yu. Shevtsova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: u_shevtsova@oparina4.ru

Junior Researcher at the Cell Technologies Laboratory

Ресей, Moscow

D. Silachev

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: d_silachev@oparina4.ru
ORCID iD: 0000-0003-0581-9755

Dr. Bio. Sci., Head of the Cell Technologies Laboratory

Ресей, Moscow

А. Ekimov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_ekimov@oparina4.ru
ORCID iD: 0000-0001-5029-0462

PhD, Head of the Laboratory of Preimplantation Genetic Testing and Genetic Diagnostics

Ресей, Moscow

N. Makarova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: np_makarova@oparina4.ru
ORCID iD: 0000-0003-1396-7272

Dr. Bio. Sci., Leading Researcher at the Prof. B.V. Leonov Department of Assistive Technologies in Infertility Treatment

Ресей, Moscow

E. Kulakova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kulakova@oparina4.ru
ORCID iD: 0000-0002-4433-4163

Dr. Med. Sci., Senior Researcher at the Prof. B.V. Leonov Department of Assistive Technologies in Infertility Treatment

Ресей, Moscow

E. Kalinina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
ORCID iD: 0000-0002-8922-2878

Dr. Med. Sci., Professor, Head of the Prof. B.V. Leonov Department of Assistive Technologies in Infertility Treatment

Ресей, Moscow

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