Efficiency analysis of IVF programs with the use of «Ural'skaya odnoshagovaya» Ural-m-1step culture medium
- Autores: Komarova Е.M.1, Kazakova I.A.2, Lisovskaya T.V.2, Ivanov A.V.3, Mayasina E.N.2, Kozhevnikova A.I.2, Ivanov I.V.4, Fedoseeva O.B.4, Vartanyan E.V.5,6,7, Levin V.A.8, Gzgzyan A.M.1, Kogan I.Y.1
-
Afiliações:
- D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
- Clinical Institute of Reproductive Medicine LLC
- City Mariinsky Hospital
- All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor
- N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia
- SRO “Association of ART Clinics"
- ART Clinic "Test Tube Children" LLC
- Ornament Health AG
- Edição: Nº 8 (2025)
- Páginas: 172-178
- Seção: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/690424
- DOI: https://doi.org/10.18565/aig.2025.194
- ID: 690424
Citar
Texto integral
Acesso aberto
Acesso está concedido
Acesso é pago ou somente para assinantes
Resumo
The procedures of the embryological stage of in vitro fertilization (IVF) program mainly determine the effectiveness of this technology for infertility treatment. Currently, in Russia it is possible to buy culture media for IVF programs from various manufacturers.
Objective: To assess the effectiveness of IVF programs with the use of "Ural'skaya odnoshagovaya" Ural-m-1step culture medium (TU 21.20.23–001–47571069–2022), LLC "Incammedic" (Russia).
Materials and methods: Two study groups were formed: the main group, which included 240 sibling IVF and IVF/ICSI protocols, with fertilization and embryo cultivation carried out on the Ural-m-1step medium (Russia), and comparison group, which consisted of 240 sibling IVF and IVF/ICSI protocols, with fertilization and embryo cultivation performed on the Vitrolife line of media (Sweden). To stimulate ovulation in patients of both groups we used protocols with GnRH antagonists; to support the luteal phase, a micronized progesterone preparation (Iprozhin, Italfarmaco, Italy/France) was introduced at a dose of 600 mg per day in 2 doses vaginally.
In the main group, 119 embryo transfers into the uterine cavity were performed on the 5th–6th day of cultivation, whereas the comparison group included 103 embryo transfers.
Results: According to the key quality indicators adopted by the Vienna and the Maribor consensuses, the effectiveness of IVF programs using the compared media did not statistically differ, p>0.05. The percentage of fertilization, the proportion of blastocysts, the number of blastocysts of excellent quality obtained on the 5th–6th day of cultivation in IVF and IVF/ICSI cycles in the main and comparison groups also did not differ. The pregnancy/implantation rate corresponded to the target ones, according to the Vienna and the Maribor consensuses, and did not have significant differences in the main and comparison groups, both in “fresh” cycles: 57/119 (54.8%) and 47/103 (48.8%), respectively, p=0.338, and in cryoprotocols: 62/119 (58.8%) and 56/103 (59.6%), respectively, p=0.660.
Conclusion: The results of the study indicate that the outcomes of IVF programs performed with in combination with the Ural-m-1step medium (TU 21.20.23–001–47571069–2022), LLC "Incammedik" (Russia), are comparable to those using the Vitrolife media (Sweden), which proves the interchangeability of these medical products in clinical practice.
Palavras-chave
Texto integral
Sobre autores
Е. Komarova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: evgmkomarova@gmail.com
ORCID ID: 0000-0002-9988-9879
PhD (Bio), Junior Researcher, embryologist, ART Department
Rússia, St. PetersburgI. Kazakova
Clinical Institute of Reproductive Medicine LLC
Email: brykina_irina@mail.ru
PhD (Bio), Head of the Clinical Embryology Laboratory
Rússia, YekaterinburgT. Lisovskaya
Clinical Institute of Reproductive Medicine LLC
Autor responsável pela correspondência
Email: tv.lis@mail.ru
ORCID ID: 0000-0002-9747-9323
Dr. Med. Sci., Associate Professor, Deputy Director General for Scientific and Methodological Work
Rússia, YekaterinburgA. Ivanov
City Mariinsky Hospital
Email: dr.ivanovav@gmail.com
Head of Assisted Reproductive Technologies Department
Rússia, St. PetersburgE. Mayasina
Clinical Institute of Reproductive Medicine LLC
Email: elena.mayasina@gmail.com
ORCID ID: 0000-0002-3387-819X
PhD, Deputy Director General for Medical Unit
Rússia, YekaterinburgA. Kozhevnikova
Clinical Institute of Reproductive Medicine LLC
Email: kkozhhh@yandex.ru
embryologist assistant
Rússia, YekaterinburgI. Ivanov
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor
Email: ministr38@gmail.com
ORCID ID: 0000-0003-0971-853X
Dr. Med. Sci., General Director
Rússia, MoscowO. Fedoseeva
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor
Email: fob18-fob18@rambler.ru
Lecturer at the Center for Scientific Research and Advanced Development
Rússia, MoscowE. Vartanyan
N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia; SRO “Association of ART Clinics"; ART Clinic "Test Tube Children" LLC
Email: emma-vartanyan@mail.ru
ORCID ID: 0000-0003-0337-086X
Dr. Med. Sci., Professor, Department of Obstetrics and Gynecology, President, Director
Rússia, Moscow; Moscow; MoscowV. Levin
Ornament Health AG
Email: vitalylevin0205@gmail.com
ORCID ID: 0009-0000-8758-0842
endocrinologist, specialist in the organization of health and public health
Suíça, LucerneA. Gzgzyan
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: agzgzyan@mail.ru
ORCID ID: 0000-0003-3917-9493
Dr. Med. Sci., Professor, Head of the ART Department
Rússia, St. PetersburgI. Kogan
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: ikogan@mail.ru
ORCID ID: 0000-0002-7351-6900
Dr. Med. Sci., Professor, Corresponding Member of RAS, Director
Rússia, St. PetersburgBibliografia
- Pivko J., Makarevich A., Olexiková L., Kubovičová E., Makovický P., Dujičková L. et al. The morphological and functional ultrastructure of cells in pre-implantation embryos. Gen. Physiol. Biophys. 2023; 42(4): 307-21. https://dx.doi.org/10.4149/gpb_2023011
- Coticchio G., Lagalla C., Sturmey R., Pennetta F., Borini A. The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART. Hum. Reprod. Update. 2019; 25(4): 422-38. https://dx.doi.org/10.1093/humupd/dmz008
- Morbeck D.E., Krisher R.L., Herrick J.R., Baumann N.A., Matern D., Moyer T. Composition of commercial media used for human embryo culture. Fertil. Steril. 2014; 102(3): 759-766.e9. https://dx.doi.org/10.1016/j.fertnstert.2014.05.043
- Morbeck D.E., Baumann N.A., Oglesbee D. Composition of single-step media used for human embryo culture. Fertil. Steril. 2017; 107(4): 1055-1060.e1. https://dx.doi.org/10.1016/j.fertnstert.2017.01.007
- Zagers M.S., Laverde M., Goddijn M., de Groot J.J., Schrauwen F.A.P., Vaz F.M. et al. The composition of commercially available human embryo culture media. Hum. Reprod. 2025; 40(1): 30-40. https://dx.doi.org/10.1093/humrep/deae248
- Zander-Fox D.L., Pacella-Ince L., Morgan D.K., Green M.P. Mammalian embryo culture media: now and into the future. Reprod. Fertil. Dev. 2023; 36(2): 66-80. https://dx.doi.org/10.1071/RD23168
- Кириенко К.В., Апрышко В.П., Харитонова М.А., Трошина М.Н., Ермилова И.Ю., Калинина Е.С., Хряпенкова Т.Г., Страшнова А.Л., Воронич Н.С., Бирюков А.А., Болт А.И., Клепуков А.А., Савина Е.М., Миронова А.Г., Симоненко Е.Ю., Яковенко С.А. Оценка эффективности программ ВРТ: есть ли необходимость применения отдельных сред для эмбрионов разных стадий развития? Проблемы репродукции. 2016; 22(5): 56-60. [Kirienko K.V., Apryshko V.P., Kharitonova M.A., Troshina M.N., Ermilova I.Yu., Kalinina E.S., Khryapenkova T.G. , Strashnova A.L., Voronich N.S., Biryukov A.A., Bolt A.I., Klepukov A.A., Savina E.M. , Mironova A.G., Simonenko E.Yu., Yakovenko S.A. Evaluation of ART program using sequential and single-step media for embryo culture? Russian Journal of Human Reproduction. 2016; 22(5): 56-60. (in Russian)]. https://doi: 10.17116/repro201622556-60
- Петросян Я.А., Сыркашева А.Г., Макарова Н.П. Влияние эмбриологического этапа на эффективность программ вспомогательных репродуктивных технологий с переносом размороженного эмбриона. Акушерство и гинекология. 2020; 4: 127-32. [Petrosyan Ya.A., Syrkasheva A.G., Makarova N.P. Impact of the embryological stage on the effectiveness of assisted reproductive technology programs with frozen-thawed embryo transfer. Obstetrics and Gynecology. 2020; (4): 127-32 (in Russian)]. https://dx.doi.org/10.18565/aig.2020.4.127-132
- Gardner D.K., Scoolcraft W.B. Inner cell mass morphology. In: Jansen R., Mortimer D., eds. In vitro culture of human blastocysts. UK: Parthenon Publishing London; 1999: 378-88.
- ESHRE Special Interest Group of Embryology and Alpha Scientists in Reproductive Medicine. Electronic address: coticchio.biogenesi@grupposandonato.it. The Vienna consensus: report of an expert meeting on the development of ART laboratory performance indicators. Reprod. Biomed. Online. 2017; 35(5): 494-510. https://dx.doi.org/10.1016/j.rbmo.2017.06.015
- ESHRE Clinic PI Working Group; Vlaisavljevic V., Apter S., Capalbo A., D'Angelo A., Gianaroli L., Griesinger G. et al. The Maribor consensus: report of an expert meeting on the development of performance indicators for clinical practice in ART. Hum. Reprod. Open. 2021; 2021(3): hoab022. https://dx.doi.org/10.1093/hropen/hoab022
Arquivos suplementares
