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New targets of Kunitz-type peptide from sea anemone Heteractis magnifica
- Authors: Kvetkina A.N. 1, Kaluzhskiy L.A. 2, Leychenko E.V. 1,3, Isaeva M.P. 1, Ivanov A.S. 2, Kozlovskaya E.P. 1
- Affiliations:
- G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences
- Institute of Biomedical Chemistry
- Far Eastern Federal University
- Issue: Vol 487, No 2 (2019)
- Pages: 221-224
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/15496
- DOI: https://doi.org/10.31857/S0869-56524872221-224
- Cite item
Abstract
The interaction of Kunitz-type peptide, HMIQ3c1, from sea anemone Heteractis magnifica with several serine proteases including inflammatory proteases was investigated using surface plasmon resonance approach. We showed that recombinant analog of HMIQ3c1 makes strong enough complexes with trypsin (KD = 1,07 × 10-9 М) and chymotrypsin (KD = 4,70 × 10-8 М). Analysis of thermodynamic parameters of HMIQ3c1/chymotrypsin revealed significant contribution of entropic factor in complex formation. The formation of specific complexes of HMIQ3c1 with neutrophil elastase (KD = 1,11 × 10-7 М) and kallikrein (KD = 2,81 × 10-8 М) indicates its anti-inflammatory activity and makes prospects to use the peptide as a potential therapeutic agent.
Keywords
About the authors
A. N. Kvetkina
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences
Author for correspondence.
Email: kvetkinaan@gmail.com
Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022
L. A. Kaluzhskiy
Institute of Biomedical Chemistry
Email: kvetkinaan@gmail.com
Russian Federation, 10, Pogodinskaya street, Moscow, 119121
E. V. Leychenko
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences; Far Eastern Federal University
Email: kvetkinaan@gmail.com
Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022; 8, Sukhanova str., Vladivostok, 690950
M. P. Isaeva
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences
Email: kvetkinaan@gmail.com
Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022
A. S. Ivanov
Institute of Biomedical Chemistry
Email: kvetkinaan@gmail.com
Russian Federation, 10, Pogodinskaya street, Moscow, 119121
E. P. Kozlovskaya
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences
Email: kvetkinaan@gmail.com
Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022
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