New targets of Kunitz-type peptide from sea anemone Heteractis magnifica

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Abstract


The interaction of Kunitz-type peptide, HMIQ3c1, from sea anemone Heteractis magnifica with several serine proteases including inflammatory proteases was investigated using surface plasmon resonance approach. We showed that recombinant analog of HMIQ3c1 makes strong enough complexes with trypsin (KD = 1,07 × 10-9 М) and chymotrypsin (KD = 4,70 × 10-8 М). Analysis of thermodynamic parameters of HMIQ3c1/chymotrypsin revealed significant contribution of entropic factor in complex formation. The formation of specific complexes of HMIQ3c1 with neutrophil elastase (KD = 1,11 × 10-7 М) and kallikrein (KD = 2,81 × 10-8 М) indicates its anti-inflammatory activity and makes prospects to use the peptide as a potential therapeutic agent.


About the authors

A. N. Kvetkina

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences

Author for correspondence.
Email: kvetkinaan@gmail.com

Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022

L. A. Kaluzhskiy

Institute of Biomedical Chemistry

Email: kvetkinaan@gmail.com

Russian Federation, 10, Pogodinskaya street, Moscow, 119121

E. V. Leychenko

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences; Far Eastern Federal University

Email: kvetkinaan@gmail.com

Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022; 8, Sukhanova str., Vladivostok, 690950

M. P. Isaeva

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences

Email: kvetkinaan@gmail.com

Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022

A. S. Ivanov

Institute of Biomedical Chemistry

Email: kvetkinaan@gmail.com

Russian Federation, 10, Pogodinskaya street, Moscow, 119121

E. P. Kozlovskaya

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences

Email: kvetkinaan@gmail.com

Russian Federation, 159, 100-let Vladivostoku prospekt, Vladivostok, 690022

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