The antiproliferative action of the “early” protein of papillomavirus HPV16 E2 on testis toumors of mice induced by the injection of Hela cells

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Abstract

The аntiploliferative effect of the “early” protein of high-risk oncogenic human papillomavirus HPV16 E2 on mice testis tumors was discovered after the indyction that was happened after the intramuscular injection of cancer HeLa cells suspension. The regression of mice testis tumors was mostly dramatic during first 1-2 days after oral vaccination with HPV16 E2 (500 mg per mice) made in tomato plant expression system that was gradually decreasing to control variant. The typical monolayer of HeLa cells on the cultivation flask bottom was appeared after seeding of testis tissue cells on the DMEM medium they were only for 18% functionally active according to the detection by the staining with nitroblue tetrazolium and trypane blue.

About the authors

R. K. Salyaev

Siberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of the Russian Academy of Sciences; Irkutsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences

Author for correspondence.
Email: salyaev@sifibr.irk.ru

Corresponding Member of the Russian Academy of Sciences

Russian Federation, 132, Lermontova str., Irkutsk, 664033; 34, Lermontov St., Irkutsk, 664033

N. I. Rekoslavskaya

Siberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of the Russian Academy of Sciences; Irkutsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences

Email: salyaev@sifibr.irk.ru
Russian Federation, 132, Lermontova str., Irkutsk, 664033; 34, Lermontov St., Irkutsk, 664033

A. S. Stolbikov

Siberian Institute of Plant Physiology and Biochemistry of the Siberian Branch of the Russian Academy of Sciences; Irkutsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences

Email: salyaev@sifibr.irk.ru
Russian Federation, 132, Lermontova str., Irkutsk, 664033; 34, Lermontov St., Irkutsk, 664033

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