DETERMINATION OF THE TOTAL SUM OF FLAVONOIDS, TANNINS AND POLYSACCHARIDE FRACTIONS IN SAPOSHNIKOVIA DIVARICATA RADICES


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Abstract

The aim of this work was to determine the total sum of flavonoids, tannins and the quantitative content of polysaccharide fractions of Saposhnikoviae divaricatae radices collected in Russia, Mongolia and obtained in China. Plant material on different phases of development was collected during field works in Tarbagataisky, Mukhorshibirsky, Ivolginsky and Kyakhtinsky districts of the Republic of Buryatia (Russia), in Aginsky district of the Transbaikal Territory (Russia) and in Khentei aimak (Mongolia), and obtained in a pharmacy store in Xining, Qinghai Province (China). The total sum of flavonoids was determined by spectrophotometric method. The content of tannins was determined by permanganatometric method in terms of tannin. The quantitative content of polysaccharide fractions was carried out by gravimetric method. The total sum of flavonoids in terms of rutin in Saposhnikoviae divaricatae radices was 0.29-0.48%. The quantitative content of tannins was ranged from 0.38 to 0.55%. Water-soluble polysaccharides were represented by an amorphous yellowish-gray powder, soluble in water. The content ranged from 6.8 to 8.3%. The fraction of pectin substances was represented by an amorphous gray powder, soluble in water. The quantitative content of the sample from Tarbagataisky region was the highest - 1.1%, while the amount of the rest ranged from 0.3 to 0.4%. The quantitative content of hemicellulose A in studied samples varied significantly, and ranged from 2.5% to 13.8%. The yield of grayish-brown powder of hemicellulose B was 1.8-14.3%. Conclusion. For the first time the content of the total sum of flavonoids, tannins and polysaccharides in the roots of Saposhnikovia divaricata from Russian and Mongolian flora was studied in this research. It was found that this species accumulates significant quantity of flavonoids and tannins along with coumarins and chromones. Relatively high content of water-soluble polysaccharides in S. divaricata shows promising studying of their composition and structure.

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About the authors

B. M Urbagarova

Baikal Institute of Nature Management Siberian branch of the Russian Academy of Sciences; Banzarov Buryat State University

Email: urbagarova.bayarma@mail.ru
Post-graduate Student, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management Siberian branch of the Russian Academy of Sciences; Lecturer, Department of Pharmacy, Banzarov Buryat State University

V. V Taraskin

Baikal Institute of Nature Management Siberian branch of the Russian Academy of Sciences; Banzarov Buryat State University

Ph.D. (Pharm.), Senior Research Scientist, Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management of the Siberian branch of the Russian Academy of Sciences; Senior Lecturer, Department of Pharmacy, Banzarov Buryat State University

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