Coupling features of intestinal and serum indole pools in obesity


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Abstract

Relevance. It is known that indole and its derivatives are formed in the intestine from tryptophan and, entering the host's body, play both a local regulatory role and a systemic effect on the body. Purpose of the study. To assess the relationship between the content of various metabolites of tryptophan metabolism in feces and blood serum in healthy individuals and in obese patients. Material and methods. We examined 266 patients with an average age of 39.9 ± 4.2 years, of which 138 were conditionally healthy, the rest were obese. The concentrations of metabolites in blood and feces were determined using the HPLC method. Results. We found that in both healthy and obese patients there is a statistically significant direct correlation between the concentration of indole-3-propionate in feces and blood. Whereas in obese patients, there is a statistically significant positive relationship between the content of indole-3-acetate in feces and serum. At the same time, in healthy patients, the levels of indole-3-acetate and an-thranilic acid in feces are interrelated, which may indicate that indole-3-acetate is normally partially converted by microorganisms into anthranilic acid. A decrease in the formation of indole-3-acetate from anthranilic acid in obese patients and, as a consequence, an increase in the absorption of indole-3-acetate and an increase in it in the blood may have a compensatory-adaptive mechanism that protects against extra-adipocytic deposition of neutral fats.

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About the authors

A. V Shestopalov

Pirogov Russian National Research Medical University

Email: al-shest@yandex.ru
Dr.Sc. (Med.), Professor Moscow, Russia

O. P Shatova

Pirogov Russian National Research Medical University

Email: shatova.op@gmail.com
Ph.D. (Med.), Associate Professor Moscow, Russia

A. A Zabolotneva

Pirogov Russian National Research Medical University

Email: a.zabolotneva@gmail.com
Ph.D. (Biol.), Associate Professor Moscow, Russia

A. M Gaponov

Dmitry Rogachev National Medical Research Centerof Pediatric, Hematology, Oncology and Immunology

Email: zorba@yandex.ru
Moscow, Russia

N. E Moskaleva

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Ph.D. (Biol.), Senior Research Scientist Moscow, Russia

S. A Appolonova

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Ph.D. (Chem.) Moscow, Russia

V. V Makarov

Center for Strategic Planning and Management of Biomedical Health Risks

Ph.D. (Biol.), Analyst Moscow, Russia

S. M Yudin

Center for Strategic Planning and Management of Biomedical Health Risks

Dr.Sc. (Med.), Professor, Director Moscow, Russia

A. G Rumyantsev

Dmitry Rogachev National Medical Research Center of Pediatric, Hematology, Oncology and Immunology; Pirogov Russian National Research Medical University

Dr.Sc. (Med.), Professor, Academician RAS, President; Honorary Professor Moscow, Russia

S. A Roumiantsev

Pirogov Russian National Research Medical University

Dr.Sc. (Med.), Professor, Corresponding Member of RAS Moscow, Russia

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