Production of interleukin-6 by resting hybridoma endothelial cells of the EA.HY926 line

Relevance. To study the mechanisms of atherogenesis, experimental models of cell culture (from the umbilical vein (HUVEC) and EA.Hy926 line cells, which are a hybrid of endotheliocytes from the umbilical vein and lung carcinoma), are widely used. The degree of effect of both individual and group cytokines on endotheliocyte physiology can be determined by adding adequate concentrations of mediators to the cell culture. However, to obtain correct results, it is necessary to determine the initial background cytokine concentration characteristic of this cell culture. The purpose of this work was to study the concentration of interleukin 6 in the culture of EA.Hy926 cells. Material and methods. The material was cell cultures of the EA.Hy926 line, which are a hybridoma of endotheliocytes from the umbilical vein (HUVEC) and lung carcinoma (A549). Results. A concentration of IL-6 was found that exceeded by more than an order of magnitude the level of this cytokine in the blood of a healthy person. Conclusion. Thus, when assessing the level of proinflammatory cytokine in the cell model, significant excesses were noted, which requires taking this factor into account during experimental work.