Identification of phenolic compounds and development of a method for quantitative determination of the amount of phenolcarboxylic acids in the herb of Phacelia Tanacetifolia benth.
- Authors: Sheykhmagomedova P.A.1, Popova O.I.1
-
Affiliations:
- Pyatigorsk Medical and Pharmaceutical Institute – Branch of FSBEI HE VolgGMU of the Ministry of Health of the Russian Federation
- Issue: Vol 25, No 12 (2022)
- Pages: 44-50
- Section: Articles
- URL: https://journals.eco-vector.com/1560-9596/article/view/321654
- DOI: https://doi.org/10.29296/25877313-2022-12-07
- ID: 321654
Cite item
Open Access
Access granted
Abstract
Relevance. Modern requirements to the quality of medicinal herbal raw materials (herbs) include the assessment of the content of active substances. Development of the method of quantitative determination of the amount of active substances is one of the stages of standardization of a new type of herbal plants.
The aim of the study is to identify phenolic compounds and to develop a method of quantitative determination of the sum of phenolcarboxylic acids as a dominant group of biologically active substances in the herb of phacelia tanacetifolia.
Material and methods. The object of research was a herb of phacelia tanacetifolia, grown in territory of Republic Dagestan in vicinities of Kaspiysk. The herb was collected during flowering in April, 2022 and dried by air-shadowing method. Phenolic compounds were studied by thin-layer chromatography on chromatographic plates "Sorbfil PTSH-P-A" (Russia, 10*15). Identification was performed in the presence of standard samples. The plates were viewed in UV light before and after treatment with 2% alcohol solution of aluminum chloride. The sum of phenolcarboxylic acids was determined by direct spectrophotometry.
Results. Phenolcarboxylic acids (chlorogenic and caffeic acids) and flavonoids (rutin and quercetin) were identified in phacelia herb. In the course of the study the optimum conditions of extraction under which the maximum yield of the sum of phenolcarboxylic acids is observed were established. The obtained spectrum of alcoholic extraction from phacelia herb had maximum absorption at wave length 330 nm that corresponds to the absorption maximum of standard sample of chlorogenic acid.
Conclusions. The presence of phenolcarboxylic acids (chlorogenic and caffeic acids) and flavonoids (rutin and quercetin) in phacelia herb has been established by the thin-layer chromatography method. The methods of quantitative determination of the sum of phenolcarboxylic acids in 70% alcohol extract of the herb of phacelia tanacetifolia recalculated to chlorogenic acid have been developed.
Full Text
About the authors
P. A. Sheykhmagomedova
Pyatigorsk Medical and Pharmaceutical Institute – Branch of FSBEI HE VolgGMU of the Ministry of Health of the Russian Federation
Author for correspondence.
Email: Patya16101998@yandex.ru
Post-gradient Student, Department of Pharmacognosy, Botany and Phytopreparation Technology
Russian Federation, PyatigorskO. I. Popova
Pyatigorsk Medical and Pharmaceutical Institute – Branch of FSBEI HE VolgGMU of the Ministry of Health of the Russian Federation
Email: Patya16101998@yandex.ru
Dr.Sc. (Pharm.), Professor, Department of Pharmacognosy, Botany and Phytopreparation Technology
Russian Federation, PyatigorskReferences
- Tokareva M.G., Prozhogina Ju.Je., Kalenikova E.I., Dzhavahjan M.A. Analiz assortimenta rossijskogo rynka lekarstvennyh rastitel'nyh preparatov sedativnogo dejstvija. Voprosy obespechenija kachestva lekarstvennyh sredstv. 2019; 2(24): 4–19.
- Gosudarstvennyj reestr lekarstvennyh sredstv [Jelektronnyj resurs]. URL: https://clck.ru/UCoGb (Data obrashhenija: 20.05.2022).
- Rybalko M.V., Kurkin V.A., Shmygareva A.A., San'kov A.N. Razrabotka sostava, metodiki poluchenija i standartizacija lekarstvennogo rastitel'nogo preparata «Mareny krasil'noj sirop». Rossijskij medicinskij zhurnal. 2020; 28(9): 77–80.
- Chibis S.P., Smaljuga N.A., Chibis V.V. Sroki poseva medonosnoj kul'tury facelii pizhmolistnoj (Phacelia tanacetifolia Benth.) v Omskoj oblasti. Raznoobraznoe i ustojchivoe razvitie agrobiocenozov Omskogo Priir-tysh'ja: Materialy Nacional'noj nauchno-prakticheskoj konferencii, posvjashhennoj 90-letiju botanicheskogo sada Omskogo GAU. 2017: 169–174.
- Shejhmagomedova P.A., Popova O.I. Fitohimicheskoe issledovanie facelii pizhmolistnoj. Materialy 67-j Vserossijskoj nauchnoj konferencii molodyh uchenyh i studentov s mezhdunarodnym uchastiem. Mahachkala: IPC DGMU. 2019: 575–578.
- Shejhmagomedova P.A. Opredelenie antocianov v cvetkah facelii pizhmolistnoj (Phacelia tanacetifolia Benth.). Razrabotka lekarstvennyh sredstv – tradicii i perspektivy: Sbornik materialov, Tomsk, 13–16 sentjabrja 2021 g. Federal'noe gosudarstvennoe bjudzhetnoe obrazovatel'noe uchrezhdenie vysshego obrazovanija «Sibirskij gosudarstvennyj medicinskij universitet» Ministerstva zdravoohranenija Rossijskoj Federacii. Tomsk: Izd-vo SibGMU. 2021: 161–163.
- Bajkacz S., Baranowska I., Buszewski B., Kowalski B., Ligor M. Determination of Flavonoids and Phenolic Acids in Plant Materials Using SLE-SPE-UHPLC-MS. MS Method Food Analytical Methods. 2018; 11: 3563–3575.
- Shejhmagomedova P.A., Popov I.V., Popova O.I. Morfologo-anatomicheskoe izuchenie travy facelii pizhmolistnoj (Phacelia tanacetifolia Benth.). Vestnik Voronezhskogo gosudarstvennogo universiteta. Serija: Himija. Biologija. Farmacija. 2021; 2: 120–127.
- Kopyt'ko Ja.F., Mizina P.G., Masese P.M., Sidel'nikov N.I. Razrabotka metodik kolichestvennogo opredelenija fenolal'degidov i fenolkarbonovyh kislot v zhidkih lekarstvennyh formah dlja naruzhnogo primenenija, soderzhashhih rastitel'nye jekstrakty. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2016; 19(8): 3–9.
- Nesterova N.V., Samylina I.A., Bobkova N.V. i dr. Kolichestvennoe opredelenie gidroksikorichnyh kislot i analiz dinamiki ih nakoplenija v list'jah jabloni lesnoj. Vestnik Moskovskogo universiteta. Serija 2: Himija. 2019; 60(1): 60–64.
- Tyheev Zh.A., Taraskin V.V., Radnaeva L.D. Kolichestvennoe soderzhanie summy fenol'nyh soedinenij v trave volodushki dvustebel'noj (Bupleurum bicaule Helm). Vestnik Burjatskogo gosudarstvennogo universiteta. Medicina i farmacija. 2021; 1: 40–51. doi: 10.18101/2306-1995-2021-1-40-51
- Kornopol'ceva T.V., Chehirova G.V., Abidueva L.R., Chukaev S.A. Standartizacija suhogo jekstrakta iz list'ev krapivy dvudomnoj i ego jeffektivnost' v profilaktike gipoksicheskih sostojanij. Butlerovskie soobshhenija. 2008; 13(3): 62–64.
- Popov I.V., Chumakova V.V., Popova O.I., Chumakov V.F. Biologicheski aktivnye veshhestva, projavljajushhie antioksidantnuju aktivnost', nekotoryh predstavitelej semejstva Lamiaceae, kul'tiviruemyh v Krasnodarskom krae. Himija rastitel'nogo syr'ja. 2019; 4: 163–172. doi: 10.14258/jcprm.2019045200
- Chistova Ju.I. Kolichestvennoe opredelenie summy gidroksikorichnyh kislot v jekstrakte sbora oduvanchika lekarstvennogo travy i lopuha bol'shogo lista suhom. Vestnik Smolenskoj gosudarstvennoj medicinskoj akademii. 2019; 18(1): 170–176.
- Tohsyrova Z.M., Popov I.V., Popova O.I. Issledovanie fenol'nyh soedinenij list'ev i pobegov rozmarina lekarstvennogo (Rosmarinus officinalis L.), introducirovannogo v botanicheskom sadu Pjatigorskogo mediko-farmacevticheskogo instituta. Himija rastitel'nogo syr'ja. 2018; 3: 199–207. doi: 10.14258/jcprm.2018033733
- Gosudarstvennaja farmakopeja Rossijskoj Federacii [Jelektronnyj resurs]: 14 izd. M: MZ RF, 2018. v 4 t. Rezhim dostupa: Federal'naja jelektronnaja medicinskaja biblioteka (femb.ru)
- Guljaev D.K., Belonogova V.D., Mashhenko P.S. Razrabotka metodiki opredelenija soderzhanija gidroksikorichnyh kislot v kornjah eli obyknovennoj. Vestnik Voronezhskogo gosudarstvennogo universiteta. Serija: Himija. Biologija. Farmacija. 2019; 2: 80–86.
- Leonova V.N., Popov I.V., Popova O.I., Zajcev V.P. Kolichestvennoe opredelenie summy fenol'nyh soedinenij v plodah Rhus typhina (L.). Himija rastitel'nogo syr'ja. 2019; 1: 225–232. doi: 10.14258/jcprm.2019014038
Supplementary files
