Apoptotic and proinflammatory processes estimation in patients with diabetic neuropathy
- Authors: Obraztsova I.A.1, Popov S.S.1, Verevkin A.N.2, Pashkova A.A.1, Kryl'skii E.D.2, Popova T.N.2
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Affiliations:
- Voronezh State Medical University named after N.N. Burdenko of the Ministry of Health of Russia
- Voronezh State University
- Issue: Vol 27, No 2 (2024)
- Pages: 3-9
- Section: Medical chemistry
- URL: https://journals.eco-vector.com/1560-9596/article/view/625796
- DOI: https://doi.org/10.29296/25877313-2024-02-01
- ID: 625796
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Abstract
Introduction. Diabetes mellitus and its complications remain one of the leading global diseases. Although there has been significant progress in diabetic peripheral neuropathy (DPN) diagnostics and treatment, the search for the molecular basis of its pathogenesis remains relevant. This search will enable targeted action on specific molecules and increase treatment effectiveness. Aim of the study. The aim of the present study was to analyse the mRNA levels of nuclear factor kappa-bi (NF-kB), apoptosis-inducing factor (AIF) and fibroblast growth factor 21 (FGF21), as well as changes in clinical and biochemical parameters in DPN patients undergoing inpatient treatment.
Material and methods. DPN patients (n=45) undergoing inpatient treatment were included in the study. Clinical and biochemical parameters were analysed on admission and before discharge. Transcript levels of the AIF and NF-kB genes were determined by real-time reverse transcription-polymerase chain reaction. FGF21 levels were determined by enzyme-linked immunosorbent assay.
Results. On admission to the hospital, the patients showed the main clinical signs of DPN. After treatment, there was a change in blood biochemical parameters towards control values (p<0.05). At the same time, there was a decrease in the mRNA level of the factors NF-kB (p=0.021) and AIF (p=0.015) in the patients' blood cells. There was an increase in FGF21 levels. Correlations were found between clinical and biochemical parameters, transcript levels of NF-kB and AIF factors and FGF21 levels.
Conclusion. The conducted studies testify about the positive influence of the therapy carried out in the hospital conditions on inflammatory and apoptotic processes, which is evidenced by the decrease in the mRNA level of the NF-κB and AIF factors genes, as well as by the increase in the concentration of FGF21, which is apparently associated with the decrease in the oxidative stress intensity.
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About the authors
I. A. Obraztsova
Voronezh State Medical University named after N.N. Burdenko of the Ministry of Health of Russia
Email: wer.all@mail.ru
Post-graduate Student, Department of Polyclinic Therapy
Russian Federation, VoronezhS. S. Popov
Voronezh State Medical University named after N.N. Burdenko of the Ministry of Health of Russia
Email: wer.all@mail.ru
Dr.Sc. (Med.), Associate Professor, Head of the Department of Organisation of Pharmaceutical Business, Clinical Pharmacy and Pharmacognosy
Russian Federation, VoronezhA. N. Verevkin
Voronezh State University
Author for correspondence.
Email: wer.all@mail.ru
Ph.D. (Biol.), Associate Professor, Department of Medical Biochemistry, Molecular and Cell Biology
Russian Federation, VoronezhA. A. Pashkova
Voronezh State Medical University named after N.N. Burdenko of the Ministry of Health of Russia
Email: wer.all@mail.ru
Dr.Sc. (Med.), Professor, Head of the Department of Polyclinic Therapy
Russian Federation, VoronezhE. D. Kryl'skii
Voronezh State University
Email: wer.all@mail.ru
Ph.D. (Biol.), Associate Professor, Department of Medical Biochemistry, Molecular and Cell Biology
Russian Federation, VoronezhT. N. Popova
Voronezh State University
Email: wer.all@mail.ru
Dr.Sc. (Biol.), Professor, Head of the Department of Medical Biochemistry, Molecular and Cell Biology
Russian Federation, VoronezhReferences
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