Selection of methodology for Thiophan M isolation from animal organs

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Abstract

Introduction. An important preparatory stage of collecting information for the registration of a new drug is the study of its pharmacokinetic parameters in accordance with the requirements of good laboratory practice. The preparatory stage of such a study consists in the selection of optimal conditions for sample preparation of biological objects to determine the test substance in them.

The purpose of this study is to select optimal conditions for sample preparation, including the pH value of the extractant, and isolation of Thiophane M from the organs of laboratory animals (rats) for its further voltammetric determination according to the previously developed method.

Material and methods. The object of the study was the substance Thiophane M [dodecyl(3,5,-dimethyl-4-hydroxybenzyl)sulfide], which is a promising biologically active compound with antioxidant properties, developed on the basis of the Department of Chemistry of Novosibirsk State Pedagogical University together with the Research Institute of Antioxidant Chemistry. Experimental data were obtained using 5 male rats weighing from 350-380 g, which were injected with an intragastrically tested sample of Thiophane M at a dose of 500 mg/kg in the form of an oil solution in olive oil.

Results. The total number of samples obtained and examined is 180. Based on the data obtained with their subsequent statistical processing by the indirect differences method according to the State Pharmacopoeia of the XIV edition, it was established that the optimal condition for the extraction of Thiophane M is a single extraction with diethyl ether, which is confirmed by the results of voltammetric analysis. We have found that the greatest number Thiophane M localized in the brain, heart and lungs (364.0±8.20, 332.0±16.47 and 275.0±25.40 µcg/g, respectively), fewer Thiophane M detected in kidney, spleen and liver (146.0±15.50, 81.0±8.66 56.0±14.53 µcg/g, respectively).

Conclusions. Of all the methods of Thiophan M isolation from rat organs, the method using diethyl ether as an extractant was chosen, which gave stable results due to the maximum extraction of the substance.

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About the authors

A. V. Ligostaev

Novosibirsk State Medical University

Author for correspondence.
Email: arbi.83@mail.ru

Ph.D. (Pharm.)

Russian Federation, Novosibirsk

Ye. A. Ivanovskaya

Novosibirsk State Medical University

Email: arbi.83@mail.ru

Dr.Sc. (Pharm.)

Russian Federation, Novosibirsk

S. V. Terentyeva

Novosibirsk State Medical University

Email: arbi.83@mail.ru

Dr.Sc. (Pharm.)

Russian Federation, Novosibirsk

L. V. Pashkova

Novosibirsk State Medical University

Email: arbi.83@mail.ru

Senior Lecturer

Russian Federation, Novosibirsk

E. Y. Zherebtcova

Novosibirsk State Medical University

Email: arbi.83@mail.ru

Senior Lecturer

Russian Federation, Novosibirsk

O. I. Prosenko

Novosibirsk State Pedagogical University

Email: arbi.83@mail.ru

Ph.D. (Chem.), Associate Professor

Russian Federation, Novosibirsk

M. P. Pitukhin

Novosibirsk Institute of Organic Chemistry named after N.N. Vorozhtsov

Email: arbi.83@mail.ru

Post-graduate Student

Russian Federation, Novosibirsk

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Supplementary files

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2. Fig 1. Study design

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3. Fig. 2. View of voltammetric curves for alkaline extraction from brain: 1 - background; 2 - 40 μL of extraction in electrolytic cell; 3 - 20 μL of Thiophan M standard sample with a concentration of 0.1 mg/mL in electrolytic cell

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4. Fig. 3. View of voltammetric curves for acid extraction from kidneys: 1 - background; 2 - 40 μL of extract in electrolytic cell; 3 - 20 μL of Thiophan M standard sample with a concentration of 0.1 mg/mL in electrolytic cell

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5. Fig. 4. View of voltammetric curves for brain ether extract: 1 - background; 2 - 40 µl of extract in electrolytic cell; 3 - 20 µl of Thiophan M standard sample with a concentration of 0.1 mg/mL in electrolytic cell

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6. Fig. 5. View of voltammetric curves for cardiac extraction using sodium tungstate solution: 1 - background; 2 -40 µl of extraction in the electrolytic cell; 3 -20 µl of Thiophan M standard sample with a concentration of 0.1 mg/mL in the electrolytic cell

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7. Fig. 6. View of voltammetric curves for extract from spleen with extractant water: 1 - background; 2 - 40 µl of extract in electrolytic cell; 3 - 20 µl of Thiophan M standard sample with 0.1 mg/mL concentration in electrolytic cell; 4 - 40 µl of Thiophan M standard sample with 0.1 mg/mL concentration in electrolytic cell

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8. Fig. 7. View of voltammetric curves for brain meal extract with alcohol: 1 - background; 2 - 40 µl of extract in electrolytic cell; 3 - 20 µl of Thiophan M standard sample with a concentration of 0.1 mg/mL in electrolytic cell

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