Pharmaceutical development of an adhesive gel based on a biodegradable natural complex
- Authors: Rytchenkov S.V.1, Poroisky S.V.1, Stepanova E.F.2, Tatarenko-Kozmina Т.Y.3,4, Pleten А.P.4
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Affiliations:
- Volgograd State Medical University of the Ministry of Health of the Russian Federation
- Pyatigorsk Medical-Pharmaceutical Institute – branch of the Volga State Medical University of the Ministry of Health of the Russian Federation
- Scientific Research Institute "Clinical Medicine named after. N. A. Semashko"
- Moscow State Medical and Dental University named after A.I. Evdokimov Ministry of Health of the Russian Federation
- Issue: Vol 27, No 5 (2024)
- Pages: 23-30
- Section: Pharmaceutical chemistry
- URL: https://journals.eco-vector.com/1560-9596/article/view/633052
- DOI: https://doi.org/10.29296/25877313-2024-05-03
- ID: 633052
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Abstract
Introduction. One of the most important issues in the development and improvement of pharmacy is the creation of original and increasing the effectiveness of existing dosage forms. At the same time, there is justifiable interest in the group of application dosage forms (ADF), which have many advantages, both biopharmaceutical and technological and economic in nature. At the same time, the most significant and promising within this group are dosage forms such as gels and films.
Purpose of the study – development of optimal composition, adhesive gel technology, pharmacological confirmation of effectiveness on a model of intestinal anastomosis.
Material and methods. To obtain an adhesive gel, compositions based on Na-CMC (0.5–1.5%), Na-alginate (0.5–1.5%) and chitosan (1–3%) were prepared. The osmotic capacity of the gels was studied using a model of dialysis through a semipermeable membrane. The adhesive properties of the gel were determined on an experimental model of intestinal anastomosis by the time of localization of the film-gel complex at the site of application. The biodegradation properties of the film-gel complex were also studied in in vitro and in vivo experiments.
Results. A 1.5% gel based on Na-CMC has a greater capacity compared to other prototypes, capable of absorbing up to 12.56% of moisture from its own weight. Gel based on Na-alginate (1.5%) absorbs up to 10.20%, and chitosan (3.0%) – up to 11.05% moisture. The “film-gel” complex based on chitosan gel (3.0%) did not migrate from the site of application within 128 hours, which indicates its satisfactory adhesive properties, since the time during which the complex was at the site of localization corresponds to the critical time the period during which coronary artery failure most often occurs. The film-gel complex biodegraded in the blood plasma of rats within 136 hours, and remained in the abdominal cavity of rats at the site of application for up to 7 days.
Conclusions. Comparative studies of various models of adhesive gels obtained on different bases were carried out: Na-CMC (1.5%), Na-alginate (1.5%) and chitosan (3.0%) and showed the advantages of a gel based on chitosan in relation to its ability to ensure film fixation at the application site.
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About the authors
S. V. Rytchenkov
Volgograd State Medical University of the Ministry of Health of the Russian Federation
Author for correspondence.
Email: rytchenkovs@gmail.com
ORCID iD: 0009-0005-7597-4138
Lecturer, Department of Disaster Medicine, Institute of Public Health named after. N.P. Grigorenko
Russian Federation, VolgogradS. V. Poroisky
Volgograd State Medical University of the Ministry of Health of the Russian Federation
Email: poroyskiy@mail.ru
ORCID iD: 0000-0001-6990-6482
Dr.Sc. (Med.), Associate Professor, Head Department of Disaster Medicine, Institute of Public Health named after. N.P. Grigorenko
Russian Federation, VolgogradE. F. Stepanova
Pyatigorsk Medical-Pharmaceutical Institute – branch of the Volga State Medical University of the Ministry of Health of the Russian Federation
Email: e.f.stepanova@mail.ru
ORCID iD: 0000-0002-4082-3330
Dr.Sc. (Pharm.), Professor, Professor of the Department of Pharmaceutical Technology with a Course in Medical Biotechnology
Russian Federation, PyatigorskТ. Y. Tatarenko-Kozmina
Scientific Research Institute "Clinical Medicine named after. N. A. Semashko"; Moscow State Medical and Dental University named after A.I. Evdokimov Ministry of Health of the Russian Federation
Email: kosmtina025@gmail.com
Dr.Sc. (Biol.), Head of Department of Medical Biology with the Fundamentals of Cellular and Molecular Biotechnology
Russian Federation, Moscow; MoscowА. P. Pleten
Moscow State Medical and Dental University named after A.I. Evdokimov Ministry of Health of the Russian Federation
Email: pleatol@mail.ru
ORCID iD: 0000-0003-4991-2150
Dr.Sc. (Biol.), Professor of the Department of Biological Chemistry
Russian Federation, MoscowReferences
- Harenko E.A., Larionova N.I., Demina N.B. Mukoadgezivnye lekarstvennye formy (obzor). Himiko-farmacev-ticheskij zhurnal. 2009; 43: 21–29.
- Strusovskaja O.G., Rytchenkov S.V. Razrabotka tehnologii biodegradiruemoj membrany dlja predotvrashhenija razvitija peritoneal'nyh spaek. Farmacija. 2023; 72(4): 45–49.
- Shaikh Т.R., Garland M.J., Woolfson A.D. Mucoadhesive drug delivery systems. Journal of pharmacy and Bioallied Sciences. 2011; 3: 89–100.
- Asjakina L.K., Garmashov S.Ju., Bulgakova A.V. Izuchenie zavisimosti vjazkosti rastvorov polisaharidov, perspektivnyh dlja sozdanija biodegradiruemyh polimerov, ot fiziko-himicheskih vozdejstvij. Novoe slovo v nauke i praktike: gipotezy i aprobacija rezul'tatov issledovanij. 2016; 22: 159–164.
- Buzlama A.V., Doba S.H., Dagir S. Razrabotka sostava gelja na osnove hitozana. Vestnik Voronezhskogo gosudarstvennogo universiteta. Serija: Himija. Biologija. Farmacija. 2021; 1: 82–87.
- Ono K., Saito Y., Yura H. Photocrosslinkable chitosan as a biological adhesive. Journal of Biomedical Materials Research: An Official Journal of the Society for Biomaterials and The Japanese Society for Biomaterials. 2000; 49: 289–295.
- Konovalova M.V., Kurek D.V., Durnev Е.А. Degradaciya in vitro pectin-hitozanovyh kriogelej. Izvestiya Ufimskogo nauchnogo centra RAN. 2016; 3: 42–45.
- Kirzhanova E.A., Hutorjanskij V.V., Balabushevich N.G. Metody analiza mukoadgezii: ot fundamental'nyh issledovanij k prakticheskomu primeneniju v razrabotke lekarstvennyh form. Razrabotka i registracija lekarstvennyh sredstv. 2014; 3: 66–80.
- Evstaf'eva T.G., Bacheva N.N., Bessonova N.S. Primenenie spektrofotometricheskogo analiza dlja ustanovlenija osmoticheskoj i transkutannoj aktivnosti novyh lekarstvennyh form «Metamiozol'» i «Fenilbutazol'». Medicinskaja nauka i obrazovanie Urala. 2018; 19: 56–62.
- Konovalova M.V., Markov P.A., Durnev E.A. Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application. Journal of biomedical materials research. Part A. 2017; 105: 547–556.
- Shahram E. Sadraie S., Kaka G. Evaluation of chitosan–gelatin films for use as postoperative adhesion barrier in rat cecum model. International Journal of Surgery. 2013; 11: 1097–102.
- Gorskij V.A., Agapov M.A., Titkov B.E. Opyt ispol'zovanija kleevoj substancii, nasyshhennoj antibakterial'nymi preparatami, v hirurgii zheludochno-kishechnogo trakta. Hirurgija. Zhurnal im. N.I. Pirogova. 2012; 4: 48–54.
- Patent 2283669 RF. Medicinskij polimernyj klej. N.V. Sirotinkin; № 200510549315; zajavl. 2005-02-21; opubl. 2006-09-20. 6 s.
- Popov V.A. Sirotinkin N.V., Golovachenko V.A. Lateksnyj tkanevyj klej i ego primenenie v hirurgii. Polimery i medicina. 2006; 1: 25–26.
- Kozlov Ju.A., Novozhilov V.A., Podkamenev A.V. Kishechnye anastomozy u novorozhdennyh s ispol'zovaniem steplerov. Hirurgija. Zhurnal im. N.I. Pirogova. 2013; 3: 66–69.
- Zaporozhec A.A. Prichiny vozniknovenija spaek brjushiny posle pervichnyh asepticheskih operacij na zheludochno-kishechnom trakte i metod ih profilaktiki. Vestnik hirurgii imeni I.I. Grekova. 2011; 170: 14–20.
- Zhuk I.G., Salmin R. M., Gajduk A.V. Sposoby profilaktiki nesostojatel'nosti mezhkishechnyh anastomozov (obzor). Zhurnal Grodnenskogo gosudarstvennogo medicinskogo universiteta. 2010; 1: 3–6.
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