Antimicrobial properties of essential oil and water-ethanolic extracts from the medicinal plant of Anthemis nobilis (Chamaemelum nobile (L.) ALL.)

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Abstract

Introduction. To date, one of the relevant areas of development of the pharmaceutical industry is the development of new drugs based on medicinal plant raw materials with a different spectrum of pharmacological action. Of particular interest are plants that have antimicrobial properties due to the presence of a complex of biologically active substances in them. Anthemis nobilis (Chamaemelum nobile L.) is one of the promising sources of essential oils and phenolic compounds, which necessitates the study of the pharmaceutical potential of this plant.

The purpose of the research is to study of the antimicrobial activity of essential oil and water-ethanolic extracts from the plant Chamaemelum nobile L. during its cultivation in the conditions of the central agroclimatic zone of the Republic of Belarus.

Material and methods. The object of the study was the dried flowers of anthemis nobilis Medicinal raw materials were harvested during the phase of mass flowering on the territory of the Republic of Belarus in the central agro-climatic zone. The antimicrobial activity of water-ethanolic extracts and essential oil of anthemis nobilis was assessed by the method of diffusion of biologically active substances into an agar nutrient medium. Yeast and gram-negative and gram-positive bacteria from the collection of microorganisms of the Department of Biotechnology of BSTU were used as test cultures: Candida albicans (АТСС 10231), Pseudomonas aeruginosa (В-126), Escherichia coli (АТСС 8739), Bacillus subtilis, Staphylococcus aureus.

Results. Based on the results obtained, both essential oils and water-ethanolic extracts from anthemis nobilis showed an antibacterial effect of varying degrees of severity. It was found that the essential oil of the studied plant shows more pronounced antimicrobial activity in the range of studied concentrations in relation to all analyzed test cultures in comparison with the essential oil of chamomile. The most pronounced antimicrobial properties of the essential oil were manifested in relation to Bacillus subtilis (MIC = 0.1±0.05) and Candida albicans (MIC = 0.07±0.02).

Water-ethanolic extraction of anthemis nobilis has an inhibitory effect only against gram-positive bacteria (S. aureus, B. subtilis) at a concentration of more than 20 mg/ml.

Conclusions. The results of the studies allow us to recommend the use of water-ethanolic extracts, as well as the essential oil of anthemis nobilis as a component in the development of formulations of phytopreparations with antimicrobial properties.

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About the authors

А. А. Klimovich

Belarusian State Technological University

Author for correspondence.
Email: anechkaf027@gmail.com
ORCID iD: 0009-0009-4461-4841
SPIN-code: 3990-5648

Graduate student, Department of Biotechnology

Belarus, Sverdlova street 13a, Minsk, 220006

О. S. Ignatovets

Belarusian State Technological University

Email: anechkaf027@gmail.com
ORCID iD: 0009-0009-1376-1777
SPIN-code: 9964-1274

Ph. D. (Biol.), Associate Professor, Department of Biotechnology

Belarus, Sverdlova street 13a, Minsk, 220006

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2. Fig. 1. Diameters of growth inhibition zones of test cultures by 5% ethanol solutions of essential oils

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3. Fig. 2. Diameters of growth inhibition zones of test cultures by 0,5% ethanol solutions of essential oils

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4. Fig. 3. Diameters of growth inhibition zones of test cultures by 0,05% ethanol solutions of essential oils

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