The content of neuropeptides in the blood plasma of Wistar rats after subchronic low-dose exposure to mercury acetate

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Introduction. Mercury poisoning has a cumulative effect. Mercury gradually accumulates in tissues and organs, which negatively affects the functions of all body systems, especially the brain.

The aim of this study was to investigate the neuropeptides content in the blood plasma of Wistar rats after subchronic low-dose mercury acetate poisoning.

Material and methods. During the study, rats was intragastrically administered mercury acetate at a dose of 4 mg/kg for 30 days. On days 30 and 44, the blood plasma of rats was tested for the levels of calcium-binding protein (S100), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), pigment epithelial-derived factor (PEDF), myelin basic protein (MBP).

Results. The concentration of S100 protein in the blood plasma of the experimental group of rats decreased by 43,9%, and the concentration of MBP increased by 172,6%. Fourteen days after the end of the toxicant administration, the concentration of protein S100 in the blood plasma of poisoned animals was significantly lower by 132,7% compared to the control group. The content of MBP increased by 59,4% and neuron-specific enolase increased by 44,6%, the concentration of neuropeptides PEDF and GFAP changed insignificantly.

Conclusions. The results obtained demonstrate that even low concentrations of mercury acetate, entering the body for a long time, affect the concentration of neuropeptides in blood plasma, which indicates negative changes in the central nervous system of rats.

Full Text

Restricted Access

About the authors

K. M. Shchepetkova

St. Petersburg State Pediatric Medical University of the Ministry of Healthcare of the Russian Federation

Author for correspondence.
Email: tesh_07@inbox.ru
ORCID iD: 0000-0002-8250-4178
SPIN-code: 2788-9060

Post-graduate Student

Russian Federation, Litovskaya str., 2, St. Petersburg, 194100

E. G. Batotsyrenova

St. Petersburg State Pediatric Medical University of the Ministry of Healthcare of the Russian Federation; The Federal State-Financed Institution Golikov Research Clinical Center of Toxicology, Federal Medical Biological Agency

Email: bkaterina2009@yandex.ru
ORCID iD: 0000-0003-3827-4579
SPIN-code: 5800-7966

Dr.Sc. (Biol.), Head of the Department, Leading Research Scientist

Russian Federation, Litovskaya str., 2, St. Petersburg, 194100; Bekhtereva str., 1, St. Petersburg, 192019

V. A. Kashuro

St. Petersburg State Pediatric Medical University of the Ministry of Healthcare of the Russian Federation; The Herzen State Pedagogical University of Russia; Saint-Petersburg State University

Email: kashuro@yandex.ru
ORCID iD: 0000-0002-7892-0048
SPIN-code: 3821-8062

Dr.Sc. (Med.), Professor, Head of the Department

Russian Federation, Litovskaya str., 2, St. Petersburg, 194100; 48 Moika Embankment, St. Petersburg, 191186; 7-9 Universitetskaya Embankment, St. Petersburg, 199034

T. Yu. Kretser

St. Petersburg State Pediatric Medical University of the Ministry of Healthcare of the Russian Federation

Email: tkropotova@mail.ru
ORCID iD: 0000-0002-8713-9704
SPIN-code: 4456-3891

Associate Professor

Russian Federation, Litovskaya str., 2, St. Petersburg, 194100

References

  1. Vertinsky A.P., Problems of environmental pollution in the Russian Federation by heavy metals. Innovacii i investicii. 2020; 1: 232–237. (In Russ.). doi: 10.1051/e3sconf/202124401006.
  2. Li F., Ma C., Zhang P. Mercury deposition, climate change and anthropogenic activities: A review. Frontiers in Earth Science. 2020; 8: 316. doi: 10.3389/feart.2020.00316.
  3. Gladyshev V. Toxic properties of mercury and its effects on the organism of animals and humans. The Scientific Heritage. 2021; 81(2): 16–22. (In Russ.). doi: 10.24412/9215-0365-2021-81-2-16-22.
  4. Carocci A., Rovito N., Sinicropi M.S. et al. Mercury toxicity and neurodegenerative effects. Rev. Environ. Contam. Toxicol. 2014; 229: 1–18. doi: 10.1007/978-3-319-03777-6_1.
  5. Chakraborty P. Mercury exposure and Alzheimer's disease in India-An imminent threat? Science of the Total Environment. 2017; 589: 232–235. doi: 10.1016/j.scitotenv.2017.02.168.
  6. Skalny A.V., Astrakhantseva E.Yu., Skalnaya M.G. et al. Socioeconomic effects of toxic metal exposure on psycho-intellectual health of children and adolescents. Mikrojelementy v medicine. 2017; 18: 3–12. (In Russ.). doi: 10.19112/2413-6174-2017-18-3-3-12.
  7. Batotsyrenova E.G., Vakunenkova O.A., Zolotoverkhaya E.A. et al. Indicators of the antioxidant system in the long-term period after acute mercury nitrate poisoning in the experiment. Toksikologicheskij vestnik. 2020; 2: 36–41. (In Russ.). doi: 10.36946/0869-7922-2020-2-35-40.
  8. Shchepetkova K.М., Batotsyrenova E.G., Litvinenko L.A. et al. Antioxidant system and lipid peroxidation in rat erythrocytes under low-dose exposure to mercury acetate. Pediatr. 2022; 13: 25–34. (In Russ.). doi: 10.17816/PED13225-34.
  9. Graves S.I.; Baker D.J. Implicating endothelial cell senescence to dysfunction in the ageing and diseased brain. Basic and Clinical Pharmacology and Toxicology. 2020; 127(2): 102–110. doi: 10.1111/bcpt.13403.
  10. Beloborodova N.V., Dmitriyeva I.B., Chernevskaya E.A. Diagnostic Value of S100B Protein in Critical Conditions. Obshhaja reanimatologija. 2011; 6: 72–76. (In Russ.). doi: 10.15360/1813-9779-2011-6-72.
  11. Rai A., Maurya S.K., Sharma R. et al. Down-regulated GFAPα: a major player in heavy metal induced astrocyte damage. Toxicology Mechanisms and Methods. 2012; 23(2): 99–107. doi: 10.3109/15376516.2012.721809.
  12. Abooshahab R., Dass C.R. The biological relevance of pigment epithelium-derived factor on the path from aging to age-related disease. Mechanisms of Ageing and Development. 2021; 196: 111478. doi: 10.1016/j.mad.2021.111478.
  13. El-Fawal H.A. Neuroantibody biomarkers: links and challenges in environmental neurodegeneration and autoimmunity. Autoimmune Diseases. 2014; 1: 340875. doi: 10.1155/2014/340875.
  14. Golmohammadi J., Jahanian-Najafabadi A., Aliomrani M. Chronic Oral Arsenic Exposure and Its Correlation with Serum S100B Concentration. Biological Trace Element Research. 2018; 189: 172–179. DOI:.10.1007/s12011-018-1463-2.
  15. Rukavishnikov V.S., Lakhman O.L., Sosedova L.M. et al. Toxic encephalopathies in distant post-contact period of occupational neurointoxications (clinical and experimental studies). Medicina truda i promyshlennaja jekologija. 2010; 10: 22–30. (In Russ.).
  16. Sosedova L.M., Kudayeva I.V., Titov E.A. et al. Morphologic and neurochemical effects in long-term period of mercurial intoxication (experimental data). Medicina truda i promyshlennaja jekologija. 2009; 1: 37–42. (In Russ.).
  17. Kister A., Kister I. Overview of myelin, major myelin lipids, and myelin-associated proteins. Frontiers in Chemistry. 2023; 10: 1041961. doi: 10.3389/fchem.2022.1041961.
  18. Branca J.J.V., Fiorillo C., Carrino D. et al. Cadmium-Induced Oxidative Stress: Focus on the Central Nervous System. Antioxidants. 2020; 9(6): 492. doi: 10.3390/antiox9060492.
  19. Shvetsov A.V., Dyuzhikova N.A., Savenko Yu.N. et al. Effect of experimental coma on protein expression of bcl-2 and caspases-3, 9 in the rat brain. Bulletin of Experimental Biology and Medicine. 2015; 160: 178–181. (In Russ.). doi: 10.1007/s10517-015-3132-1.
  20. Shchepetkova K.M., Batotsyrenova E.G., Shustov E.B. et al. Influence of Etomersol Fumarate on Cognitive Functions of Rats During Mercury Acetate Poisoning. Biomedicina. 2023; 19: 124–129. (In Russ.). doi: 10.33647/2713-0428-19-3E-124-129.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2025 Russkiy Vrach Publishing House