Effect of experimental therapy on the gas and acid–base composition of the blood in the dynamics of toxic pulmonary edema
- Authors: Torkunov P.A.1, Zemlyanoy A.V.2, Torkunova T.V.3, Shabanov P.D.4
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Affiliations:
- City Multidisciplinary Hospital No. 2
- Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical and Biological Agency
- Saint Petersburg State Pediatric Medical University
- Kirov Military Medical Academy
- Issue: Vol 14, No 4 (2023)
- Pages: 245-250
- Section: Experimental Neuropharmacology
- URL: https://journals.eco-vector.com/1606-8181/article/view/569238
- DOI: https://doi.org/10.17816/phbn569238
- ID: 569238
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Abstract
BACKGROUND: This study is part of an extended research onfinding ways to prevent and treat toxic pulmonary edema. This study aimed to examine the gas transport function, acid–base state, and gas composition of blood in the dynamics of experimental toxic pulmonary edema and the effect on them of experimental therapy with a combination of drugs that have shown effectiveness in preliminary studies.
MATERIALS AND METHODS: Toxic edema of the lungs was modeled in mice by inhalation of toxic doses of phosgene LСt50. Gas and acid–base composition parameters of the blood were determined using a gas analyzer 3 and 24 h after poisoning. Thirty minutes after poisoning, parts of the animals were injected intraperitoneally with a complex of drugs consisting of natrii dimercaptopropansulfonas (unitiol) 150 mg/kg, diclofenac sodium 35. 0 mg/kg, and аprotinin (contrikal) 250 IU/kg.
RESULTS: The results revealed 3 h after poisoning of animals, changes in the gas composition and parameters of the gas transport function in the blood were detected, and 24 h after poisoning, impaired acid-base balance was noted. The use of a therapeutic combination of drugs consisting of sodium dimercaptopropanesulfonate (unithiol), sodium diclofenac, and aprotinin (kontrikal) led to the partial normalization of the altered parameters of the blood gas composition and parameters of the blood gas transport function but was not accompanied by the normalization of the acid-base state of the blood. The formulation demonstrated a pronounced negative effect on the blood gas composition in intact animals. The treatment of pulmonary edema with the comparison drug unithiol did not result in the normalization of the gas composition and acid-base state of the poisoned animals.
CONCLUSION:The treatment of toxic pulmonary edema with a combination of medications led to an improvement in the gas composition and gas transport function of the blood.
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About the authors
Pavel A. Torkunov
City Multidisciplinary Hospital No. 2
Author for correspondence.
Email: tpa4@mail.ru
ORCID iD: 0000-0003-0491-2237
SPIN-code: 3656-7755
Dr. Sci. (Med. ), Clinical Pharmacologist
Russian Federation, 5, Uchebniy alley, Saint Petersburg, 194354Aleksandr V. Zemlyanoy
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical and Biological Agency
Email: al-zem@yandex.ru
ORCID iD: 0000-0001-8055-2291
SPIN-code: 2114-1375
Cand. Sci. (Med. ), senior research associate, head of Laboratory
Russian Federation, Kuzmolovsky settlement, Leningrad Region
Torkunova V. Torkunova
Saint Petersburg State Pediatric Medical University
Email: ovt4@mail.ru
ORCID iD: 0000-0002-8471-3854
Cand. Sci. (Biol. ), lecturer
Russian Federation, Saint PetersburgPetr D. Shabanov
Kirov Military Medical Academy
Email: pdshabanov@mail.ru
ORCID iD: 0000-0003-1464-1127
SPIN-code: 8974-7477
Dr. Sci. (Med. ), Professor, Professor of the Pharmacology Department
Russian Federation, Saint PetersburgReferences
- Ryabov GA. Sindromy kriticheskikh sostoyanii. Moscow: Meditsina; 1994. 368 p. (In Russ. )
- Tomchin AB, Kropotov AV. Proizvodnye tiomocheviny i tiosemikarbazida. Stroenie i farmakologicheskaya aktivnost’. Zashchitnoe deistvie proizvodnykh 1,2,4-tiazinoindola pri oteke legkikh. Khimiko-Farmatsevticheskii Zhurnal. 1998;(1):22–26. (In Russ. )
- Kropotov AV. Ehksperimental’nyi otek legkikh i ego farmakoprofilaktika antigipoksantami [dissertation Abstract]. Saint Petersburg; 1996. 44 p. (In Russ. )
- Kaare E. Lundstrom. The blood gas handbook. Bronshoj; 1997. 58 p.
- Komarov FI, Korovkin BF, Men’shikov VV. Biokhimicheskie issledovaniya v klinike. Leningrad: Meditsina; 1981. 408 p. (In Russ. )
- Shanin VYu. Klinicheskaya patofiziologiya. Uchebnik dlya meditsinskikh vuzov. Saint Petersburg: Spetsial’naya literatura; 1998. 569 p. (In Russ. )
- Motavkin PA, Gel’tser BI. Klinicheskaya i ehksperimental’naya patofiziologiya legkikh. Moscow: Nauka; 1998. 366 p. (In Russ. )
- Torkunov PA, Zemlyanoy AV, Varlashova MB, et al. Experimental therapy for toxic pulmonary edema caused by inhalation poisoning with nitrogen oxides. Psychopharmacology and biological narcology. 2023;14(1):62–69. (In Russ. ) DOI: 10. 17816/phbn321622
- Topunov AF, Kosmachevskaya OV. The multiplicity of hemoglobin functional forms in human organism: the modern view and practical applications. Biomics. 2018;10(3):251–267. (In Russ. ) DOI: 10. 31301/2221-6197. bmcs. 2018-34
- Suslikova MI, Gubina MI, Aleksandrov SG, et al. Izbrannye voprosy fiziologii krovi. Uchebnoe posobie. Irkutsk: IGMU; 2021. 102 p. (In Russ. )
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