STUDY OF EXPRESSION OF MMP-1 GENE IN LOCALIZED SOLERODERMIA


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Abstract

The aim of the study was to investigate the expression of the MMP-1 gene in patients with a diagnosis of localized scleroderma. Methods. The study included 12 patients with an established diagnosis of localized scleroderma. The material of the study consisted of biopsies from the affected and uninfected areas of the skin. As a control in the work, biopsies of the same patients from unaffected areas of the skin were used. The analysis was carried out by real-time PCR. The statistical processing of the results was carried out by the 2-ΔΔCt method. Results. The values of the relative expression levels of the gene were obtained in all patients. Individual analysis of each patient showed the expression level of the MMP-1 gene in scleroderma affected skin to be lowered comparatively with the visually unaffected skin (control) in the range from 4.2 times to 20.8 times. The average value of the change in the expression level of the gene was reduced by 7.6±2.6 times. Patients were divided into two groups - with a limited plaque form and a common plaque form of scleroderma. In patients with a limited form of the disease, expression of the MMP-1 gene was shown to be significantly reduced by 5.3 times, and in patients with a common form of the disease it was reduced by 11-fold times. Conclusion. A quantitative measurement of the expression of the metalloproteinase-1 gene (MMP-1) by PCR-RT was performed. Experimentally there was showed a decrease in gene expression in scleroderma affected skin compared to unaffected skin.

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About the authors

Z. A Nevozinskaya

N.I. Pirogov Russian National Research Medical University; Moscow Scientific and Practical Center for Dermatovenereology and Cosmetology

Email: marykor@bk.ru
Moscow, Russian Federation

A. G Soboleva

Centre of Theoretical Problems of Physico-Chemical Pharmacology

Moscow, Russian Federation

E. A Klimov

University Diagnostic Laboratory; Lomonosov Moscow State University

Moscow, Russian Federation

I. M Korsunskaya

Centre of Theoretical Problems of Physico-Chemical Pharmacology; Moscow Scientific and Practical Center for Dermatovenereology and Cosmetology

Moscow, Russian Federation

V. V Sobolev

Centre of Theoretical Problems of Physico-Chemical Pharmacology; I.I. Mechnikov Research Institute for Vaccines and Sera; University Diagnostic Laboratory;

Moscow, Russian Federation

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