ACTIVATING POINT MUTATION OF BRCA1/2 GENES IN AZERBAIJAN POPULATION


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Introduction. Breast cancer is the leading cancer type among woman of Azerbaijan population. Hereditary mutations of the BRCA1/2 genes are considered as high risk for appeared breast and ovarian cancers. In Azerbaijan BRCA1/2-related breast and ovarian cancer were not investigated. Aim. The study is focused to detect BRCA1/2 germline mutation-associated breast and ovarian cancer in the Azerbaijan population. Method. DNA isolation from peripheral blood samples was performed with «colon» method. Detection of BRCA1/2 mutation was conducted using allele - specifics PCR and allele - specifics primers. Results. BRCA1_5382insC, BRCA1 5622T, BRCA1_185delAG and BRCA2_ 6174T mutations were more spreading point mutations among the breast and ovarian cancer patients. BRCA2 9254 del5 mutationin BRCA2 gene could not be detected using method and primers. Conclusion. Allele - specifics PCR and allele - specifics primers that were used in this study can be effectively used for the selection of BRCA1/2-related breast and ovarian cancer.

Full Text

Restricted Access

About the authors

J. A Aliyev

National Center of Oncology

Baku, Azerbaijan

L. A Melikova

National Center of Oncology

Email: Lmelikova20027@gmail.com
Baku, Azerbaijan

E. B Mansurov

National Center of Oncology

Baku, Azerbaijan

F. K Aliyeva

National Center of Oncology

Baku, Azerbaijan

E. E Bagirova

National Center of Oncology

Baku, Azerbaijan

S. Q Mexdizade

National Center of Oncology

Baku, Azerbaijan

A. S Nadjafova

National Center of Oncology

Baku, Azerbaijan

References

  1. Алиев Д.А., Марданлы Ф.А., Ватанха С.С., Мансуров Э.Б. Эпидемиологические аспекты заболеваемости раком молочной железы в Азербайджанской Республике за период 2012-2016 гг. Azerbaijan J. of Oncology. 2017; 1: 76-8. (Aliyev J.A., Mardanli FA., Vatankha S.S., Mansurov E.B. Epidemiological aspects of the incidence of breast cancer in the Republic of Azerbaijan for the period 2012-2016. Azerbaijan Oncology J. 2017; 1: 76-8 (in Russian)]
  2. Statistical yearbook of Azerbaijan. State statistical committee of the republic of Azerbaijan. 2017; 824.
  3. Lynch H.T., Snyder C., Lynch J. Hereditary breast cancer: practical pursuit for clinical translation. Annals of Surgical Oncology. 2012; 19 (6): 1723-31.
  4. Deng C.X., Wang R.H. Roles of BRCA1 in DNA damage repair: a link between development and cancer. Human Molecular Genetics. 2003; 12 (1): 113-23.
  5. NHGRI: Breast Cancer Information Core. https://research.nhgri.nih.gov/bic/
  6. Любченко Л.Н., Батенева Е.И., Воротников И.К. Портной С.М., Крохина О.В., Соболевский В.А., Жукова Л.Г., Хайленко В.А., Тюляндин С.А. Наследственный рак молочной железы: генетическая и клиническая гетерогенность, молекулярная диагностика, хирургическая профилактика в группах риска. Успехи молекулярной онкологии. 2014; 2: 16-25.
  7. Дмитриев И.В., Сухотерин И.И., Зыбенко Т.В., Дмитриева Т.В. Мутации генов BRCA 1, BRCA 2 - будущее предикативной онкологии: обзор литературы. Маммология. 2012; 1: 13-6. (Dmitriev I.V., Sukhoterin I.I., Zibenko T.V., Dmitrieva T.V. Mutation of the genes BRCA1, BRCA2 - the future of predictive Oncology: a review of the literature. Mammalogy. 2012; 1: 13-6 (in Russian)]
  8. Hyuna Sung, Philip S. Rosenberg, Wan-Qing Chen et al. Female Breast Cancer Incidence among Asian and Western Populations: More Similar Than Expected. J. of the National Cancer Institute. 2015; 107 (7): 107.
  9. Fatemeh K., Parvin M. A comprehensive Focus on Global Spectrum of BRCA1 and BRCA2 mutations in breast cancer. BioMed Research International. 2013; 928562: 21. http://dx.doi.org/10.1155/2013/928562
  10. Pohlreich P., Michal Z., Jana S.High proportion of recurrent germline mutations in the BRCA1 gene in breast and ovarian cancer patients from the Prague area. Breast cancer research. 2005; 7 (5): 728-36.
  11. Antoniou A.C., Pharoah P.D., Narod S., Risch H.C., et al. Breast and ovarian cancer risks to carriers of the BRCA1 5382insC and 185delAG and BRCA2 6174delT mutations: a combined analysis of 22 population based studies. Letter to JMG. 2010; 42 (7): 5-7.
  12. Balci A., Huusko P., Paakkonen K. et al. Mutation analysis of BRCA1 and BRCA2 in Turkish cancer families: a novel mutation BRCA2 3414del4 found in male breast cancer. European J. of Cancer. 1999; 35 (5): 707-10.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2019 Russkiy Vrach Publishing House

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies