MOLECULAR MECHANISMS OF RETARDATION OF SKIN AGING UNDER INFLUENCE 0F HUMAN PLACENTA HYDROLYSATE


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Abstract

Introduction. Application of placenta hydrolysate in cosmetology opens a new page in anti-aging therapy. Numerous studies have confirmed the antioxidant, anti-inflammatory, anti-pigmental properties of placenta hydrolysate preparations, as well as activation of trophic function and optimization of cellular metabolism. The difference between preparation Melsmon and its analogs is the maximum safety of the drug since it does not include hormones, growth factors, enzymes, it is completely free from toxic substances. Purpose of the study. To evaluate the possibility of using the preparation Malsmon as a regulatory and geroprotective agent for the prevention of skin aging of human fibroblasts during their senescence in vitro Methods. The expression of proteins was compared by immunocytochemistry in cell cultures of human skin fibroblasts at 7th and 14th passages with the administration of preparation Melsmon and in the control group. Results. When analyzing the data of the relative area of expression of the studied markers in the «young culture» of human fibroblasts, an increase in the expression indices of Ki-67, calreticulin, sirtuin-6 andsyntaxin under the influence of the Melsmon preparation was found after the 3rd passage. Whereas in the «old culture» on the 14th passage, when placental hydrolysate (Melsmon) was added, activation of the expression of ki-67, sirtuins 1 and 6, to the same level as in the young (control) culture was observed. Conclusion. The Melsmon preparation has pronounced geroprotective properties with respect to human skin cells, which gives arguments for its further development as a means of stimulating regenerative processes in the skin and preventing its aging.

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About the authors

I. M Kvetnoy

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St.Petersburg University

Saint Petersburg, Russian Federation

A. O Drobintseva

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University

Email: anna.drobintseva@gmail.com
Saint Petersburg, Russian Federation

T. S Kleimenova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University

Saint Petersburg, Russian Federation

V. O Polyakova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St.Petersburg University

Saint Petersburg, Russian Federation

K. A Turkadze

Sechenov University

Moscow, Russian Federation

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