Protective effect of coded L-amino acids in cytostatic presence on the development of organotypic culture of tissues of different genesis


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Abstract

Introduction. The cytostatics, inhibiting the cellular proliferation, are using in clinic for the inhibition of tumor development in patients. However, the adverse events can occur because of the damage of health cells of other tissues. A delay or decrease of these effects is an actual medicine task. Purpose of the study was the investigation of effects of coded L-amino acids on a decrease of cytostatic inhibiting effects in the culture of tissues of different genesis. These amino acids are not only the structural elements of the proteins, but can participate in the regulation of the genes, controlling the cellular cycle. Methods of organotypic culture of tissues of brain cortex, muscle, bladder of Wistar line rats in presence of cytostatic cyclophosphane (CP) and also of L-amino acids were used. The combined effect of CP and L-amino acids, stimulating the cellular regeneration, the cytostatic inhibiting effect on the cellular proliferation in the tissues of different genesis decreased by 10-43%. Conclusion. The data obtained about protective effect of coded hydrophile L-amino acids by cytostatic effect creates the base for development of medications for the delay of adverse effects related to the chemotherapy.

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About the authors

Natalia Iosifovna Chalisova

St. Petersburg Institute of Bioregulation and Gerontology; Pavlov Institute of Physiology RAS

Email: ni_chalisova@mail.ru
Expert scientific worker, Doctor of biologic sciences, Professor. pr. Dinamo, 3, St. Petersburg, 197110, Russian Federation; nab. Makarova, 6, St. Petersburg, 199034, Russian Federation

Galina Anatolevna Ryzhak

St. Petersburg Institute of Bioregulation and Gerontology

Email: galina@gerontology.ru
Director Assistance of Petersburg Institute of Bioregulation and Gerontology, Doctor of biologic sciences, Professor. pr. Dinamo, 3, St. Petersburg, 197110, Russian Federation

Oleg Mikchailovich Ivko

St. Petersburg Institute of Bioregulation and Gerontology

Email: secretary@gerontology.ru
Senior scientific worker, Candidate of biologic sciences pr. Dinamo, 3, St. Petersburg, 197110, Russian Federation

Ecaterina Sergeevna Zalomaeva

St. Petersburg Institute of Bioregulation and Gerontology; Pavlov Institute of Physiology RAS; Russian State Pedagogical University A.I. Herzen

Email: 0000-0002-6005-3433
postgraduate; Assistance of Cathedra of human and animal anatomy and physiology pr. Dinamo, 3, St. Petersburg, 197110, Russian Federation; nab. Makarova, 6, St. Petersburg, 199034, Russian Federation; St. Petersburg, 191186, nab. Moiki, 48

Polina Nicolaevna Ivanova

St. Petersburg Institute of Bioregulation and Gerontology; Pavlov Institute of Physiology RAS

Email: ivanovapolina19@mail.ru
Laboratory worker pr. Dinamo, 3, St. Petersburg, 197110, Russian Federation; nab. Makarova, 6, St. Petersburg, 199034, Russian Federation

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